Cited by 2143D Printing in Biomedical ResearchMicrofluidic and Capillary Electrophoresis ApplicationsTissue Engineering and Regenerative Medicine

Long‐term maintenance of a microfluidic 3D human liver sinusoid

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Prodanov, Ljupcho, Jindal, Rohit, Bale, Shyam Sundhar, Hegde, Manjunath, McCarty, William J., Golberg, Inna, Bhushan, Abhinav, Yarmush, Martin L. +1 more·2016·Biotechnology and Bioengineering

Abstract

The development of long‐term human organotypic liver‐on‐a‐chip models for successful prediction of toxic response is one of the most important and urgent goals of the NIH/DARPA's initiative to replicate and replace chronic and acute drug testing in animals. For this purpose, we developed a microfluidic chip that consists of two microfluidic chambers separated by a porous membrane. The aim of this communication is to demonstrate the recapitulation of a liver sinusoid‐on‐a‐chip, using human cells only for a period of 28 days. Using a step‐by‐step method for building a 3D microtissue on‐a‐chip, we demonstrate that an organotypic in vitro model that reassembles the liver sinusoid microarchitecture can be maintained successfully for a period of 28 days. In addition, higher albumin synthesis (synthetic) and urea excretion (detoxification) were observed under flow compared to static cultures. This human liver‐on‐a‐chip should be further evaluated in drug‐related studies. Biotechnol. Bioeng. 2016;113: 241–246. © 2015 Wiley Periodicals, Inc.

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  • Prodanov et al. engineered a sinusoid-mimetic chip co-culturing hepatocytes, LX-2 stellate cells, and U937 macrophages under controlled perfusion, faithfully recapitulating the hepatic sinusoidal niche.

    “Prodanov et al. engineered a sinusoid-mimetic chip co-culturing hepatocytes, LX-2 stellate cells, and U937 macrophages under controlled perfusion, faithfully recapitulating the hepatic sinusoidal niche [217].”

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Authors
Prodanov, L., Jindal, R., Bale, S. S., Hegde, M., McCarty, W. J., Golberg, I., Bhushan, A., Yarmush, M. L., & Usta, O. B.
Affiliations
  • Harvard University (US)
  • Rutgers, The State University of New Jersey (US)
Corresponding author
  • Yarmush, Martin L.
  • Usta, Osman Berk
Venue
Biotechnology and Bioengineering
Publisher
Wiley
Published
2015-08-26
Volume
113
Issue
1
Pages
241-246
ISSN
  • 0006-3592 (ISSN-L)
  • 1097-0290
OpenAlex
W2159897933
Cited by
214
References
28
Citations by year
2026: 10 · 2025: 14 · 2024: 11 · 2023: 17 · 2022: 18 · 2021: 22 · 2020: 32 · 2019: 24 · 2018: 34 · 2017: 17 · 2016: 12 · 2015: 3
FWCI
10.94
Type
journal article
Language
en
Open access
Locations
  • Biotechnology and Bioengineering (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Center for Advancing Translational Sciences (UH2TR000503)
  • National Institute of Diabetes and Digestive and Kidney Diseases (F32DK098905, K99DK095984)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
liver-on-a-chip, liver sinusoids, microfluidics, 3D microtissues, human hepatocytes, long-term culture, albumin synthesis, urea excretion, drug toxicity testing, alternatives to animal testing
MeSH terms
Drug Evaluation, Preclinical, Humans, Liver, Models, Biological, Organ Culture Techniques, Time Factors, Microfluidics

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