Microfluidics for Microalgal Biotechnology
Abstract
Microalgae have expanded their roles as renewable and sustainable feedstocks for biofuel, smart nutrition, biopharmaceutical, cosmeceutical, biosensing, and space technologies. They accumulate valuable biochemical compounds from protein, carbohydrate, and lipid groups, including pigments and carotenoids. Microalgal biomass, which can be adopted for multivalorization under biorefinery settings, allows not only the production of various biofuels but also other value-added biotechnological products. However, state-of-the-art technologies are required to optimize yield, quality, and the economical aspects of both upstream and downstream processes. As such, the need to use microfluidic-based devices for both fundamental research and industrial applications of microalgae, arises due to their microscale sizes and dilute cultures. Microfluidics-based devices are superior to their competitors through their ability to perform multiple functions such as sorting and analyzing small amounts of samples (nanoliter to picoliter) with higher sensitivities. Here, we review emerging applications of microfluidic technologies on microalgal processes in cell sorting, cultivation, harvesting, and applications in biofuels, biosensing, drug delivery, and nutrition.
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Fluorescent dyes such as Nile red and BODIPY 505/515 allow quantitative assessment of lipid content in individual cells, which is critical for researching microalgae as a raw material source for biofuel production.
“Fluorescent dyes such as Nile red and BODIPY 505/515 allow a quantitative assessment of lipid content in individual cells, which is critical when researching microalgae as a raw material source for biofuel production.”
From Multi-Sensing Monitoring of the Microalgae Biomass Cultivation Systems for Biofuels and Added Value Products Synthesis—Challenges and Opportunities (2025) · cites this paper as [236]
Measurement of chlorophyll autofluorescence enables assessment of photosynthetic efficiency and overall health of microalgae.
“In addition, the measurement of chlorophyll autofluorescence enables the assessment of photosynthetic efficiency and the overall health of the microalgae [236].”
From Multi-Sensing Monitoring of the Microalgae Biomass Cultivation Systems for Biofuels and Added Value Products Synthesis—Challenges and Opportunities (2025) · cites this paper as [236]
A variety of techniques are available to harvest, separate and sort cells, including passive settling, addition of flocculants and coagulants, filtering, dissolved air and suspended air floatation, hydrocyclones, electrocoagulation, centrifuging and use of microfluidics.
“There is a variety of techniques available to the consumer ranging from passive settling, the addition of flocculants and coagulants to create biomass aggregates and expedited settling, filtering (belt filters, membrane filters, cross-filters, oscillating filters, etc.), dissolved air and suspended air floatation, hydrocyclones, electrocoagulation, centrifuging and use of microfluidics to harvest,”
From Overview and Challenges of Large-Scale Cultivation of Photosynthetic Microalgae and Cyanobacteria (2023) · cites this paper as [109]
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- Authors
- Ozdalgic, B., Ustun, M., Dabbagh, S. R., Haznedaroglu, B. Z., Kiraz, A., & Tasoglu, S.
- Affiliations
- Doğuş University (TR)
- Koç University (TR)
- Center for Life Sciences and Technologies Bogazici University Bebek Istanbul Turkey
- Department of Physics Koc University Sariyer Istanbul Turkey
- Department of Mechanical Engineering, Engineering Faculty Koc University Sariyer Istanbul Turkey
- ORCID
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2021-02-04
- Volume
- 118
- Issue
- 4
- Pages
- 1716-1734
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W3118840233
- Cited by
- 37
- References
- 131
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 7 · 2023: 7 · 2022: 9 · 2021: 4
- FWCI
- 0.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Istanbul Technical University Academic Open Archive (Istanbul Technical University) (submittedVersion)
- Funders
- Alexander von Humboldt-Stiftung (Research Fellowship for Experienced Researchers)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Innovative Microfluidic and Catalytic Techniques Innovation
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- microfluidics, microalgae, microalgal biotechnology, microfluidic cell sorting, microalgae cultivation, microalgae harvesting, biofuels, biosensing, drug delivery, microalgal biomass valorization, biorefinery, carotenoids, microalgal lipids, microalgal pigments, space biotechnology
- MeSH terms
- Biotechnology, Microfluidic Analytical Techniques, Lab-On-A-Chip Devices, Microalgae
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