Photosynthetic efficiency of Chlorella sorokiniana in a turbulently mixed short light‐path photobioreactor
Open accessAbstract
To be able to study the effect of mixing as well as any other parameter on productivity of algal cultures, we designed a lab-scale photobioreactor in which a short light path (SLP) of (12 mm) is combined with controlled mixing and aeration. Mixing is provided by rotating an inner tube in the cylindrical cultivation vessel creating Taylor vortex flow and as such mixing can be uncoupled from aeration. Gas exchange is monitored on-line to gain insight in growth and productivity. The maximal productivity, hence photosynthetic efficiency, of Chlorella sorokiniana cultures at high light intensities (1,500 micromol m(-1) s(-1)) was investigated in this Taylor vortex flow SLP photobioreactor. We performed duplicate batch experiments at three different mixing rates: 70, 110, and 140 rpm, all in the turbulent Taylor vortex flow regime. For the mixing rate of 140 rpm, we calculated a quantum requirement for oxygen evolution of 21.2 mol PAR photons per mol O(2) and a yield of biomass on light energy of 0.8 g biomass per mol PAR photons. The maximal photosynthetic efficiency was found at relatively low biomass densities (2.3 g L(-1)) at which light was just attenuated before reaching the rear of the culture. When increasing the mixing rate twofold, we only found a small increase in productivity. On the basis of these results, we conclude that the maximal productivity and photosynthetic efficiency for C. sorokiniana can be found at that biomass concentration where no significant dark zone can develop and that the influence of mixing-induced light/dark fluctuations is marginal.
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- Authors
- Kliphuis, A. M. J., de Winter, L., Vejrazka, C., Martens, D. E., Janssen, M., & Wijffels, R. H.
- Affiliations
- Wageningen University & Research (NL)
- Corresponding author
- Kliphuis, Anna M. J.
- Venue
- Biotechnology Progress
- Publisher
- Wiley
- Published
- 2010-02-19
- Volume
- 26
- Issue
- 3
- Pages
- 687-696
- ISSN
- 1520-6033 (ISSN-L)
- 8756-7938
- OpenAlex
- W2115892151
- Cited by
- 145
- References
- 45
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 7 · 2023: 9 · 2022: 4 · 2021: 8 · 2020: 11 · 2019: 10 · 2018: 9 · 2017: 7 · 2016: 16 · 2015: 12 · 2014: 10 · 2013: 15 · 2012: 4
- FWCI
- 4.77
- Type
- journal article
- Language
- en
- Open access
- green
- research.wur.nl
- Locations
- Biotechnology Progress (publishedVersion)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- Stichting voor de Technische Wetenschappen
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Keywords
- Chlorella sorokiniana, photosynthetic efficiency, Taylor vortex flow, mixing rate, algal productivity, biomass yield, light attenuation, dark zone, high light, gas exchange
- MeSH terms
- Analysis of Variance, Carbon Dioxide, Chlorella, Oxygen, Photosynthesis, Biomass, Cell Culture Techniques, Bioreactors
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