Cited by 145Algal biology and biofuel productionFluid Dynamics and MixingAquatic Ecosystems and Phytoplankton Dynamics

Photosynthetic efficiency of Chlorella sorokiniana in a turbulently mixed short light‐path photobioreactor

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Kliphuis, Anna M. J., de Winter, Lenneke, Vejrazka, Carsten, Martens, Dirk E., Janssen, Marcel, Wijffels, René H.·2010·Biotechnology Progress

Abstract

To be able to study the effect of mixing as well as any other parameter on productivity of algal cultures, we designed a lab-scale photobioreactor in which a short light path (SLP) of (12 mm) is combined with controlled mixing and aeration. Mixing is provided by rotating an inner tube in the cylindrical cultivation vessel creating Taylor vortex flow and as such mixing can be uncoupled from aeration. Gas exchange is monitored on-line to gain insight in growth and productivity. The maximal productivity, hence photosynthetic efficiency, of Chlorella sorokiniana cultures at high light intensities (1,500 micromol m(-1) s(-1)) was investigated in this Taylor vortex flow SLP photobioreactor. We performed duplicate batch experiments at three different mixing rates: 70, 110, and 140 rpm, all in the turbulent Taylor vortex flow regime. For the mixing rate of 140 rpm, we calculated a quantum requirement for oxygen evolution of 21.2 mol PAR photons per mol O(2) and a yield of biomass on light energy of 0.8 g biomass per mol PAR photons. The maximal photosynthetic efficiency was found at relatively low biomass densities (2.3 g L(-1)) at which light was just attenuated before reaching the rear of the culture. When increasing the mixing rate twofold, we only found a small increase in productivity. On the basis of these results, we conclude that the maximal productivity and photosynthetic efficiency for C. sorokiniana can be found at that biomass concentration where no significant dark zone can develop and that the influence of mixing-induced light/dark fluctuations is marginal.

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Crossref · 10.1002/btpr.379

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Authors
Kliphuis, A. M. J., de Winter, L., Vejrazka, C., Martens, D. E., Janssen, M., & Wijffels, R. H.
Affiliations
Wageningen University & Research (NL)
Corresponding author
Kliphuis, Anna M. J.
Venue
Biotechnology Progress
Publisher
Wiley
Published
2010-02-19
Volume
26
Issue
3
Pages
687-696
ISSN
  • 1520-6033 (ISSN-L)
  • 8756-7938
OpenAlex
W2115892151
Cited by
145
References
45
Citations by year
2026: 3 · 2025: 7 · 2024: 7 · 2023: 9 · 2022: 4 · 2021: 8 · 2020: 11 · 2019: 10 · 2018: 9 · 2017: 7 · 2016: 16 · 2015: 12 · 2014: 10 · 2013: 15 · 2012: 4
FWCI
4.77
Type
journal article
Language
en
Open access
Locations
  • Biotechnology Progress (publishedVersion)
  • PubMed (publishedVersion)
  • Socio-Environmental Systems Modeling (acceptedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Funders
Stichting voor de Technische Wetenschappen
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
  • Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
Keywords
Chlorella sorokiniana, photosynthetic efficiency, Taylor vortex flow, mixing rate, algal productivity, biomass yield, light attenuation, dark zone, high light, gas exchange
MeSH terms
Analysis of Variance, Carbon Dioxide, Chlorella, Oxygen, Photosynthesis, Biomass, Cell Culture Techniques, Bioreactors

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