Cited by 241Neuroscience and Neuropharmacology ResearchRegulation of Appetite and ObesityAmino Acid Enzymes and Metabolism

The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.

Distribution of vesicular glutamate transporter mRNA in rat hypothalamus

Ziegler, Dana R., Cullinan, William E., Herman, James P.·2002·Journal of Comparative Neurology

DOI 10.1002/cne.10257

Open this paper in CoChat — free account

Abstract

Two isoforms of the vesicular glutamate transporter, VGLUT1 and VGLUT2, were recently cloned and biophysically characterized. Both VGLUT1 and VGLUT2 specifically transport glutamate into synaptic vesicles, making them definitive markers for neurons using glutamate as a neurotransmitter. The present study takes advantage of the specificity of the vesicular transporters to afford the first detailed map of putative glutamatergic neurons in the rat hypothalamus. In situ hybridization analysis was used to map hypothalamic distributions of VGLUT1 and VGLUT2 mRNAs. VGLUT2 is clearly the predominant vesicular transporter mRNA found in the hypothalamus; rich expression can be documented in regions regulating energy balance (ventromedial hypothalamus), neuroendocrine function (preoptic nuclei), autonomic tone (posterior hypothalamus), and behavioral/homeostatic integration (lateral hypothalamus, mammillary nuclei). Expression of VGLUT1 is decidedly more circumspect and is confined to relatively weak labeling in lateral hypothalamic regions, neuroendocrine nuclei, and the suprachiasmatic nucleus. Importantly, dual-label analysis revealed no incidence of colocalization of VGLUT1 or VGLUT2 mRNAs in glutamic acid decarboxylase (GAD) 65-positive neurons, indicating that GABA neurons do not express either transporter. Our data support a major role for hypothalamic glutamatergic neurons in regulation of all aspects of hypothalamic function.

Integrity signals

Registry

Crossref · 10.1002/cne.10257

Cites

53 of 53 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

The Rat Brain in Stereotaxic Coordinates
Paxinos, G., & Watson, C. (1983) · Elsevier eBooks · doi:10.1016/c2009-0-63235-9 · Cited by 28,585
Central nervous system control of food intake
Schwartz, M. W., Woods, S. C., Porte, D., Seeley, R. J., & Baskin, D. G. (2000) · Nature · doi:10.1038/35007534 · Cited by 6,451
Neurocircuitry of stress: central control of the hypothalamo–pituitary–adrenocortical axis
Herman, J. P., & Cullinan, W. E. (1997) · Trends in Neurosciences · doi:10.1016/s0166-2236(96)10069-2 · Cited by 2,270
Brain Maps: Structure of the Rat Brain
Swanson, L. W. (1992) · Medical Entomology and Zoology · Cited by 1,878
Localization of neuronal and glial glutamate transporters
Rothstein, J. D., Martin, L. J., Levey, A. I., Dykes‐Hoberg, M., Jin, L., Wu, D. M., Nash, N. R., & Kuncl, R. W. (1994) · Neuron · doi:10.1016/0896-6273(94)90038-8 · Cited by 1,703
Brain maps: structure of the rat brain
Guillery, R. W. (1993) · Trends in Neurosciences · doi:10.1016/0166-2236(93)90187-q · Cited by 1,669
The Expression of Vesicular Glutamate Transporters Defines Two Classes of Excitatory Synapse
Fremeau, R. T., Troyer, M. D., Pahner, I., Nygaard, G. O., Tran, C. H., Reimer, R. J., Bellocchio, E. E., Fortin, D. L., Storm‐Mathisen, J., & Edwards, R. H. (2001) · Neuron · doi:10.1016/s0896-6273(01)00344-0 · Cited by 1,266
Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons
Takamori, S., Rhee, J. S., Rosenmund, C., & Jahn, R. (2000) · Nature · doi:10.1038/35025070 · Cited by 862
Cerebral hemisphere regulation of motivated behavior
Swanson, L. W. (2000) · Brain Research · doi:10.1016/s0006-8993(00)02905-x · Cited by 846
Regional expression of a gene encoding a neuron-specific Na+-dependent inorganic phosphate cotransporter (DNPI) in the rat forebrain
Hisano, S., Hoshi, K., Ikeda, Y., Maruyama, D., Kanemoto, M., Ichijo, H., Kojima, I., Takeda, J., & Nogami, H. (2000) · Molecular Brain Research · doi:10.1016/s0169-328x(00)00194-7 · Cited by 806
Uptake of Glutamate into Synaptic Vesicles by an Inorganic Phosphate Transporter
Bellocchio, E. E., Reimer, R. J., Fremeau, R. T., & Edwards, R. H. (2000) · Science · doi:10.1126/science.289.5481.957 · Cited by 795
Synaptic connections between layer 4 spiny neurone-layer 2/3 pyramidal cell pairs in juvenile rat barrel cortex: physiology and anatomy of interlaminar signalling within a cortical column.
Feldmeyer, D., Lübke, J., Silver, R. A., & Sakmann, B. (2002) · The Journal of Physiology · doi:10.1113/jphysiol.2001.012959 · Cited by 656
Chemoarchitecture of the Brain
Nieuwenhuys, R. (1985) · Springer eBooks · doi:10.1007/978-3-642-70426-0 · Cited by 500
Chemoarchitecture of the brain
(1987) · Comparative Biochemistry and Physiology Part A Physiology · doi:10.1016/0300-9629(87)90305-7 · Cited by 416
The hypothalamus, intrinsic connections and outflow pathways to the endocrine system in relation to the control of feeding and metabolism
Luiten, P. G. M., Horst, G. J. T., & Steffens, A. B. (1987) · Progress in Neurobiology · doi:10.1016/0301-0082(87)90004-9 · Cited by 391
Immunocytochemical localization of candidates for vesicular glutamate transporters in the rat cerebral cortex
Fujiyama, F., Furuta, T., & Kaneko, T. (2001) · The Journal of Comparative Neurology · doi:10.1002/cne.1037 · Cited by 374
The inhibitory effect of testosterone on hypothalamic-pituitary-adrenal responses to stress is mediated by the medial preoptic area
Viau, V., & Meaney, M. (1996) · Journal of Neuroscience · doi:10.1523/jneurosci.16-05-01866.1996 · Cited by 347
The Localization of the Brain-Specific Inorganic Phosphate Transporter Suggests a Specific Presynaptic Role in Glutamatergic Transmission
Bellocchio, E. E., Hu, H., Pohorille, A., Chan, J., Pickel, V. M., & Edwards, R. H. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-21-08648.1998 · Cited by 337
Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter.
Ni, B., Rosteck, P. R., Nadi, N. S., & Paul, S. M. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.12.5607 · Cited by 316
The dorsomedial hypothalamic nucleus revisited: 1986 update
Bernardis, L. L., & Bellinger, L. L. (1987) · Brain Research Reviews · doi:10.1016/0165-0173(87)90004-x · Cited by 311
The suprachiasmatic nucleus and the circadian time-keeping system revisited
van Esseveldt, L. E., Lehman, M. N., & Boer, G. J. (2000) · Brain Research Reviews · doi:10.1016/s0165-0173(00)00025-4 · Cited by 296
Molecular Cloning of a Novel Brain‐Type Na+‐Dependent Inorganic Phosphate Cotransporter
Aihara, Y., Mashima, H., Onda, H., Hisano, S., Kasuya, H., Hori, T., Yamada, S., Tomura, H., Yamada, Y., Inoue, I., et al. (2000) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2000.0742622.x · Cited by 281
Local circuit regulation of paraventricular nucleus stress integration
Herman, J. P., Tasker, J. G., Ziegler, D. R., & Cullinan, W. E. (2002) · Pharmacology Biochemistry and Behavior · doi:10.1016/s0091-3057(01)00681-5 · Cited by 274
Sleep–waking discharge of neurons in the posterior lateral hypothalamus of the albino rat
Steininger, T. L., Alam, M. N., Gong, H., Szymusiak, R. S., & McGinty, D. (1999) · Brain Research · doi:10.1016/s0006-8993(99)01648-0 · Cited by 257
Region-Specific Regulation of Glutamic Acid Decarboxylase (GAD) mRNA Expression in Central Stress Circuits
Bowers, G., Cullinan, W. E., & Herman, J. P. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-15-05938.1998 · Cited by 250
GABA and glutamate mediate rapid neurotransmission from suprachiasmatic nucleus to hypothalamic paraventricular nucleus in rat
Hermes, M. L., Coderre, E. M., Buijs, R. M., & Renaud, L. P. (1996) · The Journal of Physiology · doi:10.1113/jphysiol.1996.sp021724 · Cited by 184 · cited 2×
“…preoptic area, paraventricular nucleus, subparaventricular zone (ventral to the paraventricular nucleus), dorsomedial hypothalamic nucleus, ventromedial hypothalamic nucleus, and paraventricular thalamic nucleus (Hermes et al., 1996; Sun et al., 2000; van Esseveldt et al., 2000; Cui et al., 2001).”
Background
The Dorsomedial Hypothalamic Nucleus Revisited: 1998 Update
Bernardis, L. L., & Bellinger, L. L. (1998) · Experimental Biology and Medicine · doi:10.3181/00379727-218-44296 · Cited by 179
Effects of daytime and nighttime stress on Fos-like immunoreactivity in the paraventricular nucleus of the hypothalamus, the habenula, and the posterior paraventricular nucleus of the thalamus
Chastrette, N., Pfaff, D. W., & Gibbs, R. B. (1991) · Brain Research · doi:10.1016/0006-8993(91)91559-j · Cited by 165
The medial supramammillary nucleus, spatial learning and the frequency of hippocampal theta activity
Pan, W. X., & McNaughton, N. (1997) · Brain Research · doi:10.1016/s0006-8993(97)00431-9 · Cited by 146
Noradrenergic Excitation of Magnocellular Neurons in the Rat Hypothalamic Paraventricular Nucleus via Intranuclear Glutamatergic Circuits
Daftary, S. S., Boudaba, C., Szabó, K., & Tasker, J. G. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-24-10619.1998 · Cited by 145
Regional expression and cellular localization of the Na(+)-dependent inorganic phosphate cotransporter of rat brain
Ni, B., Wu, X., Yan, G., Wang, J., & Paul, S. M. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-08-05789.1995 · Cited by 137
Anatomical interrelationships of the medial preoptic area and other brain regions activated following male sexual behavior: A combined Fos and tract-tracing study
Coolen, L. M., Peters, H. J. P. W., & Veening, J. G. (1998) · The Journal of Comparative Neurology · doi:10.1002/(sici)1096-9861(19980803)397:3 · Cited by 136
Differentiation-associated Na+-dependent Inorganic Phosphate Cotransporter (DNPI) Is a Vesicular Glutamate Transporter in Endocrine Glutamatergic Systems
Hayashi, M., Otsuka, M., Morimoto, R., Hirota, S., Yatsushiro, S., Takeda, J., Yamamoto, A., & Moriyama, Y. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m106244200 · Cited by 119
Chemical stimulation of the dorsomedial hypothalamus elevates plasma ACTH in conscious rats
Bailey, T. W., & DiMicco, J. A. (2001) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.2001.280.1.r8 · Cited by 112
Neurochemistry : a practical approach
Turner, A. J., & Bachelard, H. S. (1997) · Cited by 107
CNS Neurotransmitters and Neuromodulators: Glutamate
Stone, T. W. (1995) · Medical Entomology and Zoology · Cited by 104
Glutamate Transport and Storage in Synaptic Vesicles
Özkan, E. D., & Ueda, T. (1998) · The Japanese Journal of Pharmacology · doi:10.1254/jjp.77.1 · Cited by 101
Glutamate and GABA mediate suprachiasmatic nucleus inputs to spinal-projecting paraventricular neurons
Cui, L.-N., Coderre, E., & Renaud, L. P. (2001) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.2001.281.4.r1283 · Cited by 99
The supramammillary nucleus: Is it necessary for the mediation of hippocampal theta rhythm?
Thinschmidt, J. S., Kinney, G. G., & Kocsis, B. (1995) · Neuroscience · doi:10.1016/0306-4522(95)00045-k · Cited by 96
Collateral projections from the supramammillary nucleus to the medial septum and hippocampus
Vertes, R. P., & McKenna, J. T. (2000) · Synapse · doi:10.1002/1098-2396(20001201)38:3 · Cited by 92
Distribution of the mRNA encoding the four dopamine D1 receptor subtypes in the brain of the european eel (Anguilla anguilla): Comparative approach to the function of D1 receptors in vertebrates
Kapsimali, M., Vidal, B., González, A., Dufour, S., & Vernier, P. (2000) · The Journal of Comparative Neurology · doi:10.1002/(sici)1096-9861(20000410)419:3 · Cited by 92
MEMORY AND THE REGION OF THE MAMMILLARY BODIES
Sziklas, V. (1998) · Progress in Neurobiology · doi:10.1016/s0301-0082(97)00064-6 · Cited by 88
Injection of muscimol into posterior hypothalamus blocks stress-induced tachycardia
Lisa, M., Marmo, E., Wible, J. H., & DiMicco, J. A. (1989) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.1989.257.1.r246 · Cited by 64
Hormonal integration of neurochemical and sensory signals governing female reproductive behavior
Etgen, A. M., Chu, H.-P., Fiber, J. M., Karkanias, G. B., & Morales, J. M. (1999) · Behavioural Brain Research · doi:10.1016/s0166-4328(99)00085-6 · Cited by 51
Injection of muscimol in dorsomedial hypothalamus and stress-induced Fos expression in paraventricular nucleus
Morin, S. M., Stotz‐Potter, E. H., & DiMicco, J. A. (2001) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.2001.280.5.r1276 · Cited by 51
Electrophysiology and pharmacology of projections from the suprachiasmatic nucleus to the ventromedial preoptic area in rat
Sun, X., Rusak, B., & Semba, K. (2000) · Neuroscience · doi:10.1016/s0306-4522(00)00170-6 · Cited by 38
Activation of brown adipose tissue thermogenesis by chemical stimulation of the posterior hypothalamus
Amir, S. (1990) · Brain Research · doi:10.1016/0006-8993(90)90145-2 · Cited by 34
GABA exerts opposite influence on warm and cold sensitive neurons in medial preoptic area in rats
Jha, S. K., Islam, F., & Mallick, B. N. (2001) · Journal of Neurobiology · doi:10.1002/neu.1058 · Cited by 32
The role of preoptic area and anterior hypothalamus and median raphe nucleus on thermoregulatory system in freely moving rats
Ishiwata, T., Hasegawa, H., Yasumatsu, M., Akano, F., Yazawa, T., Otokawa, M., & Aihara, Y. (2001) · Neuroscience Letters · doi:10.1016/s0304-3940(01)01865-1 · Cited by 31
Chapter IV AMPA, kainate and NMDA ionotropic glutamate receptor expression—an in situ hybridization atlas
Wisden, W., Seeburg, P. H., & Monyer, H. (2000) · Handbook of chemical neuroanatomy · doi:10.1016/s0924-8196(00)80045-7 · Cited by 19
Plasma corticosterone responses to lesions and stimulations of the limbic thalami nuclei, medial mammillary nucleus and cingulate cortex.
Suárez, M. M., & Ni, P. (1988) · PubMed · Cited by 13
The amino acid neurotransmitters and histamine
Feldman, R. (1997) · Neuropsychopharmacology · Cited by 7
Developmental and Hormonal Regulation of Thermosensitive Neuron Potential Activity in Rat Brain
Belugin, S., Akino, K., Takamura, N., Mine, M., Romanovsky, D., FEDOSEEV, V., Кубарко, А. И., Kosaka, M., & Yamashita, S. (1999) · Thyroid · doi:10.1089/thy.1999.9.837 · Cited by 2

Flagged first, then by citations

Research works printed but not linked: 3

Storm‐Mathisen J, 1995, CNS neurotransmitters and neuromodulators: glutamate, 1
Swanson LW, 1987, Integrated systems of the CNS, Part I: Hypothalamus, hippocampus, amygdala, retina, 1
Wisden W, 2000, Handbook of chemical neuroanatomy: Glutamate, 99

Sorted by citations

Neural regulation of endocrine and autonomic stress responses
Ulrich-Lai, Y. M., & Herman, J. P. (2009) · Nature Reviews Neuroscience · doi:10.1038/nrn2647 · Cited by 3,318
“Glutamatergic, GABAergic and peptidergic neurons are highly intermingled in this region, making it difficult to predict its net impact on stress response”
BackgroundSelf-citation
Central mechanisms of stress integration: hierarchical circuitry controlling hypothalamo–pituitary–adrenocortical responsiveness
Herman, J. P., Figueiredo, H., Mueller, N. K., Ulrich-Lai, Y., Ostrander, M. M., Choi, D. C., & Cullinan, W. E. (2003) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2003.07.001 · Cited by 1,586
Sleep state switching
Saper, C. B., Fuller, P. M., Pedersen, N. P., Lu, J., & Scammell, T. E. (2010) · Neuron · doi:10.1016/j.neuron.2010.11.032 · Cited by 1,404
“Many neurons in this region express the vesicular glutamate transporter 2 (Hur and Zaborszky, 2005; Ziegler et al., 2002) but whether these glutamatergic neurons promote arousal remains to be determined.”
Background
Leptin Action on GABAergic Neurons Prevents Obesity and Reduces Inhibitory Tone to POMC Neurons
Vong, L., Ye, C., Yang, Z., Choi, B. J., Chua, S. C., & Lowell, B. B. (2011) · Neuron · doi:10.1016/j.neuron.2011.05.028 · Cited by 1,237
Leptin directly activates SF1 neurons in the VMH, and this action by leptin is required for normal body-weight homeostasis
Dhillon, H., Zigman, J. M., Ye, C., Lee, C. E., McGovern, R. A., Tang, V., Kenny, C. D., Christiansen, L. M., White, R. D., Edelstein, E. A., et al. (2006) · Neuron · doi:10.1016/j.neuron.2005.12.021 · Cited by 791
“Based upon the heavy expression of VGLUT2 (glutamate synaptic vesicle transport protein) in the VMH and the relative absence of other neurotransmitter synaptic vesicle transport proteins in the VMH (Collin et al., 2003; Ziegler et al., 2002 ), it is likely that the majority of VMH neurons are glutamatergic.”

See all 241

Authors: Ziegler, D. R., Cullinan, W. E., & Herman, J. P.
Affiliations: University of Kentucky (US)
Marquette University (US)
Corresponding author: Herman, James P.
ORCID: Herman, James P.
Venue: Journal of Comparative Neurology
Publisher: Wiley
Published: 2002-05-21
Volume: 448
Issue: 3
Pages: 217-229
ISSN: 0021-9967 (ISSN-L)
1096-9861
DOI: 10.1002/cne.10257
OpenAlex: W1977741133
Cited by: 241
References: 53
Citations by year: 2026: 1 · 2025: 1 · 2024: 4 · 2023: 10 · 2022: 8 · 2021: 10 · 2020: 5 · 2019: 12 · 2018: 14 · 2017: 10 · 2016: 11 · 2015: 11 · 2014: 9 · 2013: 12 · 2012: 9
FWCI: 5.25
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: The Journal of Comparative Neurology (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5r29mh056577-05, 5f31mh011980-02, 5r03mh060819-03)
National Institute of Mental Health (MH 60819, MH49698, MH56577, MH 11980)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
Keywords: vesicular glutamate transporter, VGLUT1, VGLUT2, hypothalamus, glutamatergic neurons, glutamatergic neurotransmission, mRNA distribution, ventromedial hypothalamus, suprachiasmatic nucleus, lateral hypothalamus, mammillary nuclei, GABAergic neurons, energy balance, neuroendocrine function, autonomic regulation
MeSH terms: Animals, Carrier Proteins, Gene Expression Regulation, Glutamate Decarboxylase, Hypothalamus, Male, Synaptic Transmission, Neurons, RNA, Messenger, Synaptic Vesicles, Biomarkers, Rats, Sprague-Dawley, In Situ Hybridization, Glutamic Acid, Membrane Transport Proteins, Vesicular Transport Proteins, Vesicular Glutamate Transport Protein 1, Vesicular Glutamate Transport Protein 2, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Distribution of vesicular glutamate transporter mRNA in rat hypothalamus

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks