Analysis of a polymorphic microRNA target site in the purinergic receptor P2RX7 gene
Abstract
The recent discovery of post‐transcriptional regulation by microRNAs (miRNAs) drew our attention to SNPs of putative miRNA target sites in candidate genes of depression‐related psychiatric disorders. The P2RX7 (purinergic receptor P2X, ligand‐gated ion channel, 7) gene has been suggested as a candidate for major depressive and bipolar disorder, because of repeated associations with the rs2230912 (Gln460Arg) polymorphism. As this polymorphism is located at the end of the coding region, we considered a possible linkage with SNP(s) in putative miRNA target sites of the 3′ untranslated region. Based on our in silico search, the rs1653625 fulfilled this criterion. This SNP, however, is surrounded with polycytosine and polyadenine tracts, which hindered its analysis until now. In this study, we describe a readily applicable genotyping method for rs1653625 by applying a primer that introduces mismatched nucleotides to create a restriction enzyme cleavage site. The resulting allele‐specific products with 19 base pair difference were separated by both traditional horizontal agarose gel electrophoresis and multicapillary gel electrophoresis. The developed genotyping method was applied in our depression‐related association study.
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A handful of studies have identified depression risk variants in precursor miRNA encoding genes, miRNA target genes, or genes involved in miRNA processing.
“A handful of studies have also identified depression risk variants in precursor miRNA encoding genes, 44 , 45 variants located in or near miRNA target genes 46 , 47 or variants located in genes involved in miRNA processing.”
From Epigenetics and depression (2019) · cites this paper as [46]
Cites
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Brites, D., & Fernandes, A. (2015) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2015.00476 · Cited by 599 · see citing works
“…of depression with altered miRNAs was described for P2RX7 (purinergic receptor P2x, ligand-gated ion channel 7) gene, where single nucleotide polymorphisms (SNPs) were identified in putative miRNA target sites of miR-1302 and miR-625 within the P2RX7 3′-untranslated region (Rahman et al., 2010).”
BackgroundPenner-Goeke, S., & Binder, E. B. (2019) · Dialogues in Clinical Neuroscience · doi:10.31887/dcns.2019.21.4/ebinder · Cited by 254 · see citing works
Sluyter, R. (2017) · Advances in Experimental Medicine and Biology · doi:10.1007/5584_2017_59 · Cited by 235 · see citing works
Significance of P2X7 Receptor Variants to Human Health and Disease
Sluyter, R., & Stokes, L. (2011) · Recent Patents on DNA & Gene Sequences · doi:10.2174/187221511794839219 · Cited by 147 · see citing works
Associations between depression severity and purinergic receptor P2RX7 gene polymorphisms
Halmai, Z., Dome, P., Vereczkei, A., Abdul-Rahman, O., Szekely, A., Gonda, X., Faludi, G., Sasvari-Szekely, M., & Nemoda, Z. (2013) · Journal of Affective Disorders · doi:10.1016/j.jad.2013.02.033 · Cited by 55 · see citing works
Self-citationLack of Association of P2RX7 Gene rs2230912 Polymorphism with Mood Disorders: A Meta-Analysis
Feng, W.-P., Zhang, B., Li, W., & Liu, J. (2014) · PLoS ONE · doi:10.1371/journal.pone.0088575 · Cited by 48 · see citing works
Kongsui, R., Beynon, S. B., Johnson, S. J., Mayhew, J., Kuter, P., Nilsson, M., & Walker, F. R. (2014) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2014.05.017 · Cited by 29 · see citing works
- Authors
- Rahman, O. A., Sasvari‐Szekely, M., Szekely, A., Faludi, G., Guttman, A., & Nemoda, Z.
- Affiliations
- Semmelweis University (HU)
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 2010-05-26
- Volume
- 31
- Issue
- 11
- Pages
- 1790-1795
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W1935099637
- Cited by
- 28
- References
- 2
- Citations by year
- 2025: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 3 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 8 · 2013: 5
- FWCI
- 1.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Electrophoresis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Associations and Epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- P2X7 receptor, microRNA binding sites, 3' untranslated region, single nucleotide polymorphisms, restriction enzyme cleavage sites, major depressive disorder, bipolar disorder, post-transcriptional regulation
- MeSH terms
- Base Sequence, Cloning, Molecular, Computer Simulation, Depression, DNA Mutational Analysis, Genotype, Humans, Molecular Sequence Data, Polymorphism, Restriction Fragment Length, Reproducibility of Results, Linkage Disequilibrium, Chi-Square Distribution, Polymerase Chain Reaction, Receptors, Purinergic P2, Electrophoresis, Capillary, Base Pair Mismatch, Polymorphism, Single Nucleotide, MicroRNAs, Receptors, Purinergic P2X7
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