Cited by 81Algal biology and biofuel productionPhase Equilibria and ThermodynamicsSeaweed-derived Bioactive Compounds

Extraction of astaxanthin from Haematococcus pluvialis by supercritical carbon dioxide fluid with ethanol modifier

Pan, Jian‐Liang, Wang, Hui‐Min, Chen, Chun‐Yen, Chang, Jo‐Shu·2012·Engineering in Life Sciences

Abstract

Conventional solvent extraction methods cannot attain high‐quality antioxidant extracts from microalgae and also require solvent recovery and posttreatment. In this study, we utilized environmental friendly supercritical carbon dioxide fluid extraction (SFE‐CO 2 ) techniques to obtain pigment (i.e. astaxanthin) from H aematococcus pluvialis . The effects of key operating parameters on the extraction efficiency of astaxanthin were investigated, giving an optimal condition of H . pluvialis weight, 6.5 g; CO 2 ‐flow rate, 6.0 NL/min; extraction time, 20 min; extraction pressure, 4500 psi; volume of ethanol modifier added, 9.23 mL/g; extraction temperature, 50°C; modifier composition, 99.5%. Under these optimum conditions, the astaxanthin yield was 73.9% (10.92 mg/g dry H . pluvialis powder) after eight cycle of extraction cycles. The saponification index (C S /C 0 , representing the ratio of astaxanthin concentration after and before the saponification procedures) of the extract could be increased from 1 to 12.78 by saponification with 3.5 M NaOH.

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Crossref · 10.1002/elsc.201100157

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Authors
Pan, J., Wang, H., Chen, C., & Chang, J.
Affiliations
  • Kao Yuan University (TW)
  • Department of Fragrance and Cosmetic Science Kaohsiung Medical University Kaohsiung Taiwan
  • National Cheng Kung University (TW)
Venue
Engineering in Life Sciences
Publisher
Wiley
Published
2012-07-09
Volume
12
Issue
6
Pages
638-647
ISSN
  • 1618-0240 (ISSN-L)
  • 1618-2863
OpenAlex
W1951464793
Cited by
81
References
38
Citations by year
2026: 1 · 2025: 8 · 2024: 1 · 2023: 5 · 2022: 6 · 2021: 9 · 2020: 9 · 2019: 9 · 2018: 11 · 2017: 4 · 2016: 8 · 2015: 4 · 2014: 5 · 2013: 1
FWCI
1.23
Type
journal article
Language
en
Open access
closed
Locations
Engineering in Life Sciences (publishedVersion)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Physical Sciences › Engineering › Biomedical Engineering › Phase Equilibria and Thermodynamics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
Keywords
astaxanthin, Haematococcus pluvialis, supercritical CO2 extraction, ethanol modifier, saponification, antioxidant extracts, extraction optimization, carotenoids

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