The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
High-resolution DNA melting analysis: advancements and limitations
Open accessAbstract
Recent advances in fluorescent dyes, methods, instruments and software for DNA melting analysis have created versatile new tools for variant scanning and genotyping. High resolution melting analysis (HRM or HRMA) is faster, simpler, and less expensive than alternative approaches requiring separations or labeled probes. With the addition of a saturating dye before PCR followed by rapid melting analysis of the PCR products, the sensitivity of heterozygote scanning approaches 100%. Specificity can be increased by identifying common polymorphisms with small amplicon melting, unlabeled probes or snapback primers to decrease the sequencing burden. However, some homozygotes require mixing for identification. Furthermore, different heterozygotes may produce melting curves so similar to each other that, although they clearly vary from homozygous variants, they are not differentiated from each other. Nevertheless, the experimental return for minimal effort is great. This focus issue of Human Mutation includes a concise, timely review on high resolution melting, a comparison to denaturing gradient gel electrophoresis, integration with qPCR for copy number assessment, combined amplicon scanning and unlabeled probe genotyping from a single melting curve, and applications to the mitochondrial genome and to BRCA1.
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Cites
Continuous Fluorescence Monitoring of Rapid Cycle DNA Amplification
Wittwer, C. T., Herrmann, M. G., Moss, A. A., & Rasmussen, R. P. (2013) · BioTechniques · doi:10.2144/000114043 · Cited by 1,274
Self-citationHigh-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen
Wittwer, C. T., Reed, G. H., Gundry, C. N., Vandersteen, J. G., & Pryor, R. J. (2003) · Clinical Chemistry · doi:10.1373/49.6.853 · Cited by 1,257
Self-citationContinuous Fluorescence Monitoring of Rapid Cycle DNA Amplification
Wittwer, C. T., Herrmann, M. G., Moss, A. A., & Rasmussen, R. P. (1997) · BioTechniques · doi:10.2144/97221bi01 · Cited by 1,143
Self-citationHigh-Resolution DNA Melting Analysis for Simple and Efficient Molecular Diagnostics
Reed, G. H., Kent, J. O., & Wittwer, C. T. (2007) · Pharmacogenomics · doi:10.2217/14622416.8.6.597 · Cited by 678
Self-citationGenotyping of Single-Nucleotide Polymorphisms by High-Resolution Melting of Small Amplicons
Liew, M., Pryor, R., Palais, R., Meadows, C., Erali, M., Lyon, E., & Wittwer, C. (2004) · Clinical Chemistry · doi:10.1373/clinchem.2004.032136 · Cited by 660
Self-citationReed, G. H., & Wittwer, C. T. (2004) · Clinical Chemistry · doi:10.1373/clinchem.2003.029751 · Cited by 531
Self-citationHigh-Resolution Melting Analysis (HRMA)-More than just sequence variant screening
Vossen, R. H. A. M., Aten, E., Roos, A., & den Dunnen, J. T. (2009) · Human Mutation · doi:10.1002/humu.21019 · Cited by 484
High resolution melting applications for clinical laboratory medicine
Erali, M., Voelkerding, K. V., & Wittwer, C. T. (2008) · Experimental and Molecular Pathology · doi:10.1016/j.yexmp.2008.03.012 · Cited by 282
Self-citationHerrmann, M. G., Durtschi, J. D., Bromley, L. K., Wittwer, C. T., & Voelkerding, K. V. (2006) · Clinical Chemistry · doi:10.1373/clinchem.2005.063438 · Cited by 279
Self-citationvan der Stoep, N., van Paridon, C. D. M., Janssens, T., Křenková, P., Štambergová, A., Maçek, M., Matthijs, G., & Bakker, E. (2009) · Human Mutation · doi:10.1002/humu.21004 · Cited by 135
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Castéra, L., Krieger, S., Rousselin, A., Legros, A., Baumann, J.-J., Bruet, O., Brault, B., Fouillet, R., Goardon, N., Letac, O., et al. (2014) · European Journal of Human Genetics · doi:10.1038/ejhg.2014.16 · Cited by 262
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Metadata
- Authors
- Wittwer, C. T.
- Venue
- Human Mutation
- Publisher
- Hindawi Limited
- Published
- 2009-06
- Volume
- 30
- Issue
- 6
- Pages
- 857-859
- ISSN
- 1059-7794 (ISSN-L)
- 1098-1004
- OpenAlex
- W2036450516
- Cited by
- 468
- References
- 14
- FWCI
- 9.55
- Type
- journal article
- Language
- en
- Open access
- bronze
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