Cited by 651Cancer-related Molecular PathwaysHeat shock proteins researchUbiquitin and proteasome pathways

The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.

MDMX: a novel p53‐binding protein with some functional properties of MDM2.

Open access

Shvarts, A., Steegenga, W. T., Riteco, N., van Laar, T., Dekker, P., Bazuine, M., van Ham, R. C., van der Houven van Oordt, W., Hateboer, G., van der Eb, A. J., Jochemsen, A. G.·1996·The EMBO Journal

DOI 10.1002/j.1460-2075.1996.tb00919.xPMC PMC452278

Open this paper in CoChat — free account

Abstract

Here we report the isolation of a cDNA encoding a new p53-associating protein. This new protein has been called MDMX on the basis of its structural similarity to MDM2, which is especially notable in the p53-binding domain. In addition, the putative metal binding domains in the C-terminal part of MDM2 are completely conserved in MDMX. The middle part of the MDMX and MDM2 proteins shows a low degree of conservation. We can show by co-immunoprecipitation that the MDMX protein interacts specifically with p53 in vivo. This interaction probably occurs with the N-terminal part of p53, because the activity of the transcription activation domain of p53 was inhibited by co-transfection of MDMX. Northern blotting showed that MDMX, like MDM2, is expressed in all tissues tested, and that several mRNAs for MDMX can be detected. Interestingly, the level of MDMX mRNA is unchanged after UV irradiation, in contrast to MDM2 transcription. This observation suggests that MDMX may be a differently regulated modifier of p53 activity in comparison with MDM2. Our study indicates that at least one additional member of the MDM protein family exists which can modulate p53 function.

Integrity signals

Registry

Crossref · 10.1002/j.1460-2075.1996.tb00919.x

Cites

61 of 61 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

p53 Mutations in Human Cancers
Hollstein, M., Sidransky, D., Vogelstein, B., & Harris, C. C. (1991) · Science · doi:10.1126/science.1905840 · Cited by 8,018
p53, guardian of the genome
Lane, D. P. (1992) · Nature · doi:10.1038/358015a0 · Cited by 5,138
Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours
Donehower, L. A., Harvey, M., Slagle, B. L., McArthur, M. J., Montgomery, C. A., Butel, J. S., & Bradley, A. (1992) · Nature · doi:10.1038/356215a0 · Cited by 4,649
The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53
Scheffner, M., Werness, B. A., Huibregtse, J. M., Levine, A. J., & Howley, P. M. (1990) · Cell · doi:10.1016/0092-8674(90)90409-8 · Cited by 3,995
Germ Line p53 Mutations in a Familial Syndrome of Breast Cancer, Sarcomas, and Other Neoplasms
Malkin, D., Li, F. P., Strong, L. C., Fraumeni, J. F., Nelson, C. E., Kim, D. H., Kassel, J., Gryka, M. A., Bischoff, F. Z., Tainsky, M. A., et al. (1990) · Science · doi:10.1126/science.1978757 · Cited by 3,765
The mdm-2 oncogene product forms a complex with the p53 protein and inhibits p53-mediated transactivation
Momand, J., Zambetti, G. P., Olson, D. C., George, D., & Levine, A. J. (1992) · Cell · doi:10.1016/0092-8674(92)90644-r · Cited by 3,113
Association of Human Papillomavirus Types 16 and 18 E6 Proteins with p53
Werness, B. A., Levine, A. J., & Howley, P. M. (1990) · Science · doi:10.1126/science.2157286 · Cited by 2,562
T antigen is bound to a host protein in SY40-transformed cells
Lane, D. P., & Crawford, L. V. (1979) · Nature · doi:10.1038/278261a0 · Cited by 2,456
The P-loop — a common motif in ATP- and GTP-binding proteins
Saraste, M., Sibbald, P. R., & Wittinghofer, A. (1990) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(90)90281-f · Cited by 2,099
Amplification of a gene encoding a p53-associated protein in human sarcomas
Oliner, J. D., Kinzler, K. W., Meltzer, P. S., George, D. L., & Vogelstein, B. (1992) · Nature · doi:10.1038/358080a0 · Cited by 1,949
Characterization of a 54K Dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells
Linzer, D. I. H., & Levine, A. J. (1979) · Cell · doi:10.1016/0092-8674(79)90293-9 · Cited by 1,814
The p53-mdm-2 autoregulatory feedback loop.
Wu, X., Bayle, J. H., Olson, D., & Levine, A. J. (1993) · Genes & Development · doi:10.1101/gad.7.7a.1126 · Cited by 1,801
Suppression of Human Colorectal Carcinoma Cell Growth by Wild-Type p53
Baker, S. J., Markowitz, S. D., Fearon, E. R., Willson, J. K. V., & Vogelstein, B. (1990) · Science · doi:10.1126/science.2144057 · Cited by 1,742
Oncoprotein MDM2 conceals the activation domain of tumour suppressor p53
Oliner, J. D., Pietenpol, J. A., Thiagalingam, S., Gyuris, J., Kinzler, K. W., & Vogelstein, B. (1993) · Nature · doi:10.1038/362857a0 · Cited by 1,474
Rescue of early embryonic lethality in mdm2-deficient mice by deletion of p53
de Oca Luna, R. M., Wagner, D. S., & Lozano, G. (1995) · Nature · doi:10.1038/378203a0 · Cited by 1,420
mdm2 expression is induced by wild type p53 activity.
Barak, Y., Juven, T., Haffner, R., & Oren, M. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb05678.x · Cited by 1,290
Rescue of embryonic lethality in Mdm2-deficient mice by absence of p53
Jones, S. N., Roe, A. E., Donehower, L. A., & Bradley, A. (1995) · Nature · doi:10.1038/378206a0 · Cited by 1,280
Mice deficient for Rb are nonviable and show defects in neurogenesis and haematopoiesis
Lee, E. Y.-H. P., Chang, C.-Y., Hu, N., Wang, Y.-C. J., Lai, C., Herrup, K., Lee, W., & Bradley, A. (1992) · Nature · doi:10.1038/359288a0 · Cited by 1,264
A major segment of the neurofibromatosis type 1 gene: cDNA sequence, genomic structure, and point mutations
Cawthon, R., Weiss, R. B., Xu, G., Viskochil, D. H., Culver, M., Stevens, J., Robertson, M. A., Dunn, D. M., Gesteland, R. F., O'Connell, P., et al. (1990) · Cell · doi:10.1016/0092-8674(90)90253-b · Cited by 1,093
Database of p53 gene somatic mutations in human tumors and cell lines.
Hollstein, M. C., Rice, K., Greenblatt, M. S., Soussi, T., Fuchs, R., Sørlie, T., Hovig, E. J., Smith‐Sørensen, B., Montesano, R., & Harris, C. C. (1994) · Europe PMC (PubMed Central) · Cited by 858
Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells
Sarnow, P., Ho, Y.-S., Williams, J., & Levine, A. J. (1982) · Cell · doi:10.1016/0092-8674(82)90356-7 · Cited by 836
Presence of a Potent Transcription Activating Sequence in the p53 Protein
Fields, S., & Jang, S. K. (1990) · Science · doi:10.1126/science.2144363 · Cited by 790
Tumorigenic potential associated with enhanced expression of a gene that is amplified in a mouse tumor cell line.
Fakharzadeh, S. S., Trusko, S. P., & George, D. L. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb07676.x · Cited by 704
Mad: A heterodimeric partner for Max that antagonizes Myc transcriptional activity
Ayer, D. E., Kretzner, L., & Eisenman, R. N. (1993) · Cell · doi:10.1016/0092-8674(93)90661-9 · Cited by 697
Two distinct mechanisms alter p53 in breast cancer: mutation and nuclear exclusion.
Moll, U. M., RIOU, G. F., & Levine, A. J. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.15.7262 · Cited by 572
Amplification and overexpression of the MDM2 gene in a subset of human malignant gliomas without p53 mutations.
Reifenberger, G., Liu, L., Ichimura, K., Schmidt, E., & Collins, V. P. (1993) · PubMed · Cited by 562
Wild-type p53 binds to the TATA-binding protein and represses transcription.
Seto, E., Usheva, A., Zambetti, G. P., Momand, J. A., Horikoshi, N., Weinmann, R. S., Levine, A. J., & Shenk, T. E. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.24.12028 · Cited by 504
Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein
Martin, K., Trouche, D., Hagemeier, C., Sørensen, T. S., Thangue, N. B. L., & Kouzarides, T. (1995) · Nature · doi:10.1038/375691a0 · Cited by 454
Wild-type p53 protein undergoes cytoplasmic sequestration in undifferentiated neuroblastomas but not in differentiated tumors.
Moll, U. M., LaQuaglia, M. P., Bénard, J., & RIOU, G. F. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.10.4407 · Cited by 428
Functional inactivation but not structural mutation of p53 causes liver cancer
Ueda, H., Ullrich, S. J., Gangemi, J. D., Kappel, C. A., Ngo, L. T., Feitelson, M. A., & Jay, G. (1995) · Nature Genetics · doi:10.1038/ng0195-41 · Cited by 368
Wild type p53 can mediate sequence-specific transactivation of an internal promoter within the mdm2 gene.
Juven‐Gershon, T., Barak, Y., Zauberman, A., George, D. L., & Oren, M. (1993) · PubMed · Cited by 332
Regulation of mdm2 expression by p53: alternative promoters produce transcripts with nonidentical translation potential.
Barak, Y., Gottlieb, E., Juven-Gershon, T., & Oren, M. (1994) · Genes & Development · doi:10.1101/gad.8.15.1739 · Cited by 328
Physical and functional interaction between WT1 and p53 proteins.
Maheswaran, S., Park, S., Bernard, A., Morris, J. F., Rauscher, F. J., HILL, D. E., & Haber, D. A. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.11.5100 · Cited by 307
Hepatitis B x antigen and p53 are associated in vitro and in liver tissues from patients with primary hepatocellular carcinoma.
Feitelson, M. A., Zhu, M., Duan, L., & London, W. T. (1993) · PubMed · Cited by 307
Human TAFII31 protein is a transcriptional coactivator of the p53 protein.
Lu, H., & Levine, A. J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.11.5154 · Cited by 301
Regulation of the Human hsp70 Promoter by p53
Agoff, S. N., Hou, J., Linzer, D. I., & Wu, B. (1993) · Science · doi:10.1126/science.8418500 · Cited by 292
The human MDM-2 oncogene is overexpressed in leukemias
Bueso-Ramos, C., Yang, Y. S., deLeon, E., McCown, P., Stass, S. A., & Albitar, M. X. (1993) · Blood · doi:10.1182/blood.v82.9.2617.bloodjournal8292617 · Cited by 292
A functional p53-responsive intronic promoter is contained within the human mdm2 gene
Zauberman, A., Flusberg, D., Haupt, Y., Barak, Y., & Oren, M. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.14.2584 · Cited by 276
The mdm-2 gene is induced in response to UV light in a p53-dependent manner.
Perry, M. E., Piette, J., Zawadzki, J., Harvey, D., & Levine, A. J. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.24.11623 · Cited by 247
Regulation of Transcription Functions of the p53 Tumor Suppressor by the mdm-2 Oncogene
Chen, J., Lin, J., & Levine, A. J. (1995) · Molecular Medicine · doi:10.1007/bf03401562 · Cited by 214
What the papers say: The p53‐mdm2 autoregulatory feedback loop: A paradigm for the regulation of growth control by p53?
Picksley, S. M., & Lane, D. P. (1993) · BioEssays · doi:10.1002/bies.950151008 · Cited by 207
Altered Patterns of MDM2 and TP53 Expression in Human Bladder Cancer
LIANES, P., Orlow, I., Zhang, Z., Oliva, M. R., Sarkis, A. S., Reuter, V. E., & Cordon‐Cardo, C. (1994) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/86.17.1325 · Cited by 194
Enhanced translation: a novel mechanism of mdm2 oncogene overexpression identified in human tumor cells.
Landers, J. E., Haines, D. S., STRAUSS, J. F., & George, D. L. (1994) · PubMed · Cited by 143
Physical and Functional Interaction between Wild-Type p53 and mdm2 Proteins
Haines, D. S., Landers, J. E., Engle, L. J., & George, D. L. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.2.1171 · Cited by 136
Adenovirus Serotype Determines Association and Localization of the Large E1B Tumor Antigen with Cellular Tumor Antigen p53 in Transformed Cells
ZANTEMA, A., Schrier, P. I., Davis-Olivier, A., van Laar, T., Vaessen, R. T. M. J., & van der Eb, A. J. (1985) · Molecular and Cellular Biology · doi:10.1128/mcb.5.11.3084 · Cited by 131
Overexpression of the MDM2 gene by childhood acute lymphoblastic leukemia cells expressing the wild-type p53 gene
Zhou, M., Yeager, A., Smith, S. D., & Findley, H. W. (1995) · Blood · doi:10.1182/blood.v85.6.1608.bloodjournal8561608 · Cited by 129
Enhanced binding of a 95 kDa protein to p53 in cells undergoing p53‐mediated growth arrest.
Barak, Y., & Oren, M. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05270.x · Cited by 113
p53 binds to the TATA-binding protein-TATA complex
Martin, D. W., Muñoz, R. M., Subler, M. A., & Deb, S. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)38619-3 · Cited by 106
BS69, a novel adenovirus E1A‐associated protein that inhibits E1A transactivation.
Hateboer, G., Gennissen, A., Ramos, Y. F., Kerkhoven, R. M., Sonntag-Buck, V., Stunnenberg, H. G., & Bernards, R. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb07318.x · Cited by 97
Self-citation
Oncogene Amplification in Urothelial Cancers With p53 Gene Mutation or MDM2 Amplification
Habuchi, T., Kinoshita, H., Yamada, H., Kakehi, Y., Ogawa, O., Wu, W., Takahashi, R., Sugiyama, T., & Yoshida, O. (1994) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/86.17.1331 · Cited by 96
Physical and functional interactions between p53 and cell cycle co‐operating transcription factors, E2F1 and DP1.
O’Connor, D. J., Lam, E. W. ‐., Griffin, S., Zhong, S., Leighton, L. C., Burbidge, S. A., & Lü, X. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb00309.x · Cited by 94
The p53-associated protein MDM2 contains a newly characterized zinc-binding domain called the RING finger
Boddy, M. N., Freemont, P. S., & Borden, K. L. B. (1994) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(94)90020-5 · Cited by 91
Adenovirus Serotype Determines Association and Localization of the Large E1B Tumor Antigen with Cellular Tumor Antigen p53 in Transformed Cells
ZANTEMA, A., Schrier, P. I., Davis-Olivier, A., et al. (1985) · Molecular and Cellular Biology · doi:10.1128/mcb.5.11.3084-3091.1985 · Cited by 69
The Tumor Suppressor p53 and the Oncoprotein Simian Virus 40 T Antigen Bind to Overlapping Domains on the MDM2 Protein
Brown, D. R., Deb, S., Muñoz, R. M., Subler, M. A., & Deb, S. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.11.6849 · Cited by 58
The status of the p53 gene in human papilloma virus positive or negative cervical carcinoma cell lines
Srivastava, S. K., Tong, Y. A., Devadas, K., Zou, Z., Chen, Y., Pirollo, K. F., & Chang, E. H. (1992) · Carcinogenesis · doi:10.1093/carcin/13.7.1273 · Cited by 47
MDM2 overexpression does not account for stabilization of wild-type p53 protein in non-Hodgkin's lymphomas
Maestro, R., Gloghini, A., Doglioni, C., Gasparotto, D., Vukosavljevic, T., Re, V. D., Laurino, L., Carbone, A., & Boiocchi, M. (1995) · Blood · doi:10.1182/blood.v85.11.3239.bloodjournal85113239 · Cited by 35
Cytogenetic evolution of mycn and mdm2 amplification in the neuroblastoma ls tumour and its cell line
Corvi, R., Savelyeva, L., Amler, L. C., Handgretinger, R., & Schwab, M. (1995) · European Journal of Cancer · doi:10.1016/0959-8049(95)00031-d · Cited by 31
Distinct Modulation of p53 Activity in Transcription and Cell-Cycle Regulation by the Large (54 kDa) and Small (21 kDa) Adenovirus E1B Proteins
Steegenga, W. T., van Laar, T., Shvarts, A., Terleth, C., van der Eb, A. J., & Jochemsen, A. G. (1995) · Virology · doi:10.1006/viro.1995.1512 · Cited by 28
Self-citation
The Tumor Suppressor p53 and the Oncoprotein Simian Virus 40 T Antigen Bind to Overlapping Domains on the MDM2 Protein
Brown, D. R., Deb, S., Muñoz, R. M., et al. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.11.6849-6857.1993 · Cited by 22
GM1492 Human Diploid Skin Fibroblasts Lack the p53-Dependent G1 Cell-Cycle Checkpoint
Vanlaar, T., Steegenga, W. T., Jochemsen, A. G., Terleth, C., & VANDEREB, A. (1995) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1995.2839 · Cited by 13
Self-citation
Human chromosome 11 suppresses the tumorigenicity of adenovirus transformed baby rat kidney cells: Involvement of the wilms' tumor 1 gene
Menke, A. L., van Ham, R. C. A., Sonneveld, E., Shvarts, A., Stanbridge, E. J., Miyagawa, K., van der Eb, A. J., & Jochemsen, A. G. (1995) · International Journal of Cancer · doi:10.1002/ijc.2910630115 · Cited by 7
Self-citation

Sorted by citations

The first 30 years of p53: growing ever more complex.
Levine, A. J., & Oren, M. (2009) · Nature Reviews Cancer · doi:10.1038/nrc2723 · Cited by 1,967
Malignant astrocytic glioma: genetics, biology, and paths to treatment
Furnari, F. B., Fenton, T., Bachoo, R. M., Mukasa, A., Stommel, J. M., Stegh, A., Hahn, W. C., Ligon, K. L., Louis, D. N., Brennan, C., et al. (2007) · Genes & Development · doi:10.1101/gad.1596707 · Cited by 2,316
Regulating the p53 pathway: in vitro hypotheses, in vivo veritas
Toledo, F., & Wahl, G. M. (2006) · Nature reviews. Cancer · doi:10.1038/nrc2012 · Cited by 1,286
Mutant p53: one name, many proteinsPreprint
Freed-Pastor, W. A., & Prives, C. L. (2012) · Genes & Development · doi:10.1101/gad.190678.112 · Cited by 1,290
The MDM2-p53 interaction.
Moll, U. M., & Petrenko, O. (2003) · PubMed · Cited by 778

See all 651

Authors: Shvarts, A., Steegenga, W. T., Riteco, N., van Laar, T., Dekker, P., Bazuine, M., van Ham, R. C., van der Houven van Oordt, W., Hateboer, G., van der Eb, A. J., & Jochemsen, A. G.
Affiliations: Leiden University (NL)
ORCID: Steegenga, W. T.
Dekker, P.
Jochemsen, A. G.
Venue: The EMBO Journal
Publisher: Springer Science and Business Media LLC
Published: 1996-10-01
Volume: 15
Issue: 19
Pages: 5349-5357
ISSN: 0261-4189 (ISSN-L)
1460-2075
DOI: 10.1002/j.1460-2075.1996.tb00919.x
PMC: PMC452278
OpenAlex: W1592021885
Cited by: 648
References: 59
Citations by year: 2026: 14 · 2025: 13 · 2024: 14 · 2023: 23 · 2022: 26 · 2021: 20 · 2020: 26 · 2019: 21 · 2018: 19 · 2017: 24 · 2016: 39 · 2015: 16 · 2014: 40 · 2013: 25 · 2012: 36
FWCI: 4.05
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: The EMBO Journal (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Data Archiving and Networked Services (DANS) (submittedVersion)
Topics: Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
Keywords: MDMX, MDM2, p53, p53-binding protein, protein-protein interaction, metal-binding domain, p53 regulation, cDNA cloning
MeSH terms: Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cloning, Molecular, Humans, Molecular Sequence Data, Nuclear Proteins, Organ Specificity, Protein Binding, Proto-Oncogene Proteins, Recombinant Fusion Proteins, RNA, Messenger, Transcription, Genetic, Transfection, Ultraviolet Rays, Transcriptional Activation, Tumor Suppressor Protein p53, Sequence Homology, Amino Acid, Sequence Analysis, DNA, DNA, Complementary, Mice, Proto-Oncogene Proteins c-mdm2

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

MDMX: a novel p53‐binding protein with some functional properties of MDM2.

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks