Ultrastructural localization of RFamide‐like peptides in neuronal dense‐cored vesicles in the peduncle of Hydra
Abstract
The presence of Arg-Phe-amide (RFamide)-like peptides in dense-cored vesicles in neurons of the peduncle of Hydra was demonstrated by immunogold electron microscopy. Thin sections of Lowicryl-embedded tissue labeled with antisera to RFamide and 5-nm gold-conjugated, secondary antibody and of Epon-Araldite-embedded tissue labeled with 15-nm gold particles revealed a concentration of RFamide-like immunoreactivity over the granular cores of vesicles in epidermal ganglion cells. Gold-labeled, dense-cored vesicles were present in the perikaryon, long thin neurites, and axon terminals of these neurons. The aggregation of labeled dense-cored vesicles in an axon terminal on the myoneme of an epitheliomuscular cell suggests a possible function of RFamide-like peptides in neuromuscular transmission. Gold staining of dense-cored vesicles completely disappeared when the RFamide antiserum was preabsorbed with 10 micrograms/ml RFamide. These results are the first demonstration that the dense-cored vesicles of Hydra neurons contain a neuropeptide.
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Pharmacological evidence and the localization of RFamide neuropeptides and ionotropic RFamide receptors suggest that iRFa-Rs are involved in neuromuscular transmission in Hydra.
“The presence of RFamide neuropeptides in large-dense core vesicles at the neuromuscular junction [58] and of the ionotropic RFamide-receptors (iRFa-Rs) in epitheliomuscular cells together with pharmacological evidence suggests that the iRFa-Rs are involved in neuromuscular transmission [39,40].”
From Back to the Basics: Cnidarians Start to Fire (2017) · cites this paper as [58]
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Self-citation
- Authors
- Koizumi, O., Wilson, J. D., Grimmelikhuijzen, C. J. P., & Westfall, J. A.
- Affiliations
- Kansas State University (US)
- Corresponding author
- Koizumi, Osamu
- Venue
- Journal of Experimental Zoology
- Publisher
- Wiley
- Published
- 1989-01
- Volume
- 249
- Issue
- 1
- Pages
- 17-22
- ISSN
- 0022-104X (ISSN-L)
- 1097-010X
- OpenAlex
- W2115661200
- Cited by
- 67
- References
- 24
- Citations by year
- 2025: 2 · 2023: 2 · 2022: 1 · 2021: 2 · 2017: 3 · 2016: 3 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 7.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Experimental Zoology (publishedVersion)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (NS-10264)
- Topics
- Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- neuropeptides, dense core vesicles, Hydra, axon terminals, neuromuscular transmission, immunogold electron microscopy, ultrastructural localization, epitheliomuscular cells, myonemes
- MeSH terms
- Animals, Hydra, Immunohistochemistry, Microscopy, Electron, Neuropeptides
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