Capillary electrophoresis with Orbitrap-Velos mass spectrometry detection

Wojcik, Roza; Li, Yihan; MacCoss, Michael J.; Dovichi, Norman J.

doi:10.1016/j.talanta.2011.10.048

Cited by 43Mass Spectrometry Techniques and ApplicationsAdvanced Proteomics Techniques and ApplicationsMicrofluidic and Capillary Electrophoresis Applications

Capillary electrophoresis with Orbitrap-Velos mass spectrometry detection

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Wojcik, Roza, Li, Yihan, MacCoss, Michael J., Dovichi, Norman J.·2012·Talanta

DOI 10.1016/j.talanta.2011.10.048PMC PMC3266533

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Abstract

Capillary electrophoresis can provide fast and efficient separations of peptides. However, the high speed separation and limited loading capacity of capillary electrophoresis requires the use of a fast and sensitive detector. While laser-induced fluorescence provides exquisite sensitivity and millisecond response time, it inherently generates a low information content signal. In contrast, mass spectrometry provides an information rich signal that is attractive for peptide analysis. The recently introduced Velos-Orbitrap mass spectrometer is capable of fast and sensitive tandem MS acquisition and simultaneous high accuracy MS acquisition, which is well suited for coupling with fast and efficient separation methods for peptide analysis. We evaluated this instrument as a detector for peptide separation by capillary electrophoresis. In MS mode, we observed low attomole detection limits for a number of peptides in a tryptic digest of standard proteins with high mass resolution (30,000 at m/z 400). The response time of the Orbitrap at this resolution was ∼0.70s, which was adequate to reconstruct the peak shape and area of our electrophoretic peaks. The linear ion-trap successfully recorded tandem MS spectra of tryptic peptides at 20 nM concentration.

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The use of an ion trap storage/reflectron time-of-flight mass spectrometer as a rapid and sensitive mass and tandem mass detector for capillary separations.
Wu, J. (1997) · Deep Blue (University of Michigan)
Capillary electrophoresis/tandem mass spectrometry with array detection
Deterding, L. J., Tomer, K. B., Wellemans, J. M. Y., Cerny, R. L., & Gross, M. L. (1999) · European Journal of Mass Spectrometry · doi:10.1255/ejms.247 · Cited by 5 · see citing works

Authors: Wojcik, R., Li, Y., MacCoss, M. J., & Dovichi, N. J.
Affiliations: University of Notre Dame (US)
University of Washington (US)
ORCID: Wojcik, Roza
Li, Yihan
MacCoss, Michael J.
Venue: Talanta
Publisher: Elsevier BV
Published: 2012-01
Volume: 88
Pages: 324-329
ISSN: 0039-9140 (ISSN-L)
DOI: 10.1016/j.talanta.2011.10.048
PMC: PMC3266533
OpenAlex: W2029739770
Cited by: 43
References: 39
Citations by year: 2025: 1 · 2022: 1 · 2019: 2 · 2018: 3 · 2017: 3 · 2016: 3 · 2015: 3 · 2014: 6 · 2013: 14 · 2012: 6
FWCI: 3.91
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Talanta (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: National Institutes of Health (R01GM096767)
Topics: Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Keywords: capillary electrophoresis, mass spectrometry detection, peptide separation, tandem mass spectrometry, linear ion trap, tryptic peptides, detection limit, mass resolution, response time (sensor)
MeSH terms: Molecular Weight, Peptides, Saccharomyces cerevisiae, Sensitivity and Specificity, Time Factors, Electrophoresis, Capillary, Saccharomyces cerevisiae Proteins, Tandem Mass Spectrometry

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Capillary electrophoresis with Orbitrap-Velos mass spectrometry detection

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