Regulation of human neural precursor cells by laminin and integrins
Abstract
Deciphering the factors that regulate human neural stem cells will greatly aid in their use as models of development and as therapeutic agents. The extracellular matrix (ECM) is a component of stem cell niches in vivo and regulates multiple functions in diverse cell types, yet little is known about its effects on human neural stem/precursor cells (NSPCs). We therefore plated human NSPCs on four different substrates (poly‐L‐ornithine, fibronectin, laminin, and matrigel) and compared their responses with those of mouse NSPCs. Compared with the other substrates, laminin matrices enhanced NSPC migration, expansion, differentiation into neurons and astrocytes, and elongation of neurites from NSPC‐derived neurons. Laminin had a similar spectrum of effects on both human and mouse cells, highlighting the evolutionary conservation of NSPC regulation by this component of the ECM. Flow cytometry revealed that human NSPCs express on their cell surfaces the laminin‐binding integrins α3, α6, α7, β1, and β4, and function‐blocking antibodies to the α6 subunit confirmed a role for integrins in laminin‐dependent migration of human NSPCs. These results define laminin and its integrin receptors as key regulators of human NSPCs. © 2006 Wiley‐Liss, Inc.
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Matrigel's multi-component bioactivity facilitates neurite outgrowth, synapse formation, and network maturation, key DNT endpoints.
“This multi-component bioactivity facilitates neurite outgrowth, synapse formation, and network maturation, key DNT endpoints, explaining its widespread adoption despite known batch-to-batch variability (Kleinman et al., 1986; Flanagan et al., 2006; Ma et al., 2008; Hughes et al., 2010; Li et al., 2014; Long and Huttner, 2019).”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Flanagan et al., 2006
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- Authors
- Flanagan, L. A., Rebaza, L. M., Derzic, S., Schwartz, P. H., & Monuki, E. S.
- Venue
- Journal of Neuroscience Research
- Publisher
- Wiley
- Published
- 2006-02-13
- Volume
- 83
- Issue
- 5
- Pages
- 845-856
- ISSN
- 0360-4012
- 1097-4547
- Cited by
- 243
- References
- 3
- Language
- en
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