The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Piperine, an alkaloid from black pepper, inhibits growth of human colon cancer cells via G1 arrest and apoptosis triggered by endoplasmic reticulum stress
Abstract
Piperine, a piperidine alkaloid present in black pepper, inhibits the growth of cancer cells, although the mechanism of action is not well understood. In this study, we show that piperine (75–150 µM) inhibited the growth of several colon cancer cell lines but had little effect on the growth of normal fibroblasts and epithelial cells. Piperine inhibited HT‐29 colon carcinoma cell proliferation by causing G1 phase cell cycle arrest that was associated with decreased expression of cyclins D1 and D3 and their activating partner cyclin‐dependent kinases 4 and 6, as well as reduced phosphorylation of the retinoblastoma protein and up‐regulation of p21/WAF1 and p27/KIP1 expression. In addition, piperine caused hydroxyl radical production and apoptosis that was partially dependent on the production of reactive oxygen species. Piperine‐treated HT‐29 cells showed loss of mitochondrial membrane integrity and cleavage of poly (ADP‐ribose) polymerase‐1, as well as caspase activation and reduced apoptosis in the presence of the pan‐caspase inhibitor zVAD‐FMK. Increased expression of the endoplasmic reticulum stress‐associated proteins inositol‐requiring 1α protein, C/EBP homologous protein, and binding immunoglobulin protein, and activation of c‐Jun N‐terminal kinase and p38 mitogen‐activated protein kinase, as well as decreased phosphorylation of Akt and reduced survivin expression were also observed in piperine‐treated HT‐29 cells. Furthermore, piperine inhibited colony formation by HT‐29 cells, as well as the growth of HT‐29 spheroids. Cell cycle arrest and endoplasmic reticulum stress‐associated apoptosis following piperine treatment of HT‐29 cells provides the first evidence that piperine may be useful in the treatment of colon cancer. © 2014 Wiley Periodicals, Inc.
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Cites
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“The pan-caspase inhibitor zVAD-FMK, the c-Jun N-terminal kinase (JNK) inhibitor SP600125, and the p38mitogen-activated protein kinase (MAPK) inhibitor SB203580 were purchased from EMD Biosciences (San Diego, CA).”
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“Western blot analysis of lysates from piperine-treated HT-29 cells showed an increase in cytochrome c release from mitochondria, indicating loss of mitochondrial membrane integrity that is often associated with apoptosis induction [34] (Figure 5B).”
BackgroundTRB3: A tribbles Homolog That Inhibits Akt/PKB Activation by Insulin in Liver
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Piperine: A review of its biological effects
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Current and emerging therapeutic approaches for colorectal cancer: A comprehensive review
Kumar, A., Gautam, V., Sandhu, A., Rawat, K., Sharma, A., & Saha, L. (2023) · World Journal of Gastrointestinal Surgery · doi:10.4240/wjgs.v15.i4.495 · Cited by 272
Herbs that heal: Nature’s pharmacy endowed remedies for better health
Mehrotra, N. (2021) · Annals of Phytomedicine: An International Journal · doi:10.21276/ap.2021.10.1.2 · Cited by 27
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Metadata
- Authors
- Yaffe, P. B., Power Coombs, M. R., Doucette, C. D., Walsh, M., & Hoskin, D. W.
- Venue
- Molecular Carcinogenesis
- Publisher
- Wiley
- Published
- 2014-05-13
- Volume
- 54
- Issue
- 10
- Pages
- 1070-1085
- ISSN
- 0899-1987 (ISSN-L)
- 1098-2744
- OpenAlex
- W2157011225
- Cited by
- 188
- References
- 9
- FWCI
- 8.06
- Type
- journal article
- Language
- en
- Open access
- closed
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