Cited by 76Antioxidant Activity and Oxidative StressPhytochemicals and Antioxidant ActivitiesExercise and Physiological Responses

β-Carotene and β-cryptoxanthin but not lutein evoke redox and immune changes in RAW264 murine macrophages

Katsuura, Sakurako, Imamura, Tomomi, Bando, Noriko, Yamanishi, Rintaro·2009·Molecular Nutrition & Food Research

Abstract

The mechanism of immunological benefits induced by carotenoids has not been fully elucidated. Here, we investigated some of the immunity-related properties of beta-carotene and two other carotenoids, beta-cryptoxanthin, and lutein, on the murine macrophages cell line RAW264. beta-Carotene added to the culture medium accumulated in the cells in a time- and dose-dependent manner. The accumulation was positively correlated with cellular lipid peroxidation, demonstrating the pro-oxidative activity of beta-carotene, and also with the synthesis of glutathione, an intracellular antioxidant. Conversely, accumulation of beta-carotene was negatively correlated with the transcription of immune-active molecules, such as IL-1beta, IL-6, and IL-12 p40, in cells stimulated by LPS and INF-gamma. The transcription of the pro-inflammatory cytokines IL-1beta and IL-6 was more sensitive to the accumulation of beta-carotene than was IL-12 p40. The accumulation of beta-cryptoxanthin in cells resulted in effects similar to those of beta-carotene. However, lutein accumulated minimally and did not significantly affect the cells. These results demonstrate that beta-carotene, and beta-cryptoxanthin as well, can accumulate in RAW264 cells and induce changes in intracellular redox status, which in turn regulate the immune function of macrophages.

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Crossref · 10.1002/mnfr.200800566

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  • β-carotene inhibited expression of inflammatory genes in LPS-stimulated RAW264.7 cells and LPS-treated mice.

    “and inhibited the expression of the inflammatory genes in lipopolysaccharide (LPS) stimulated RAW264.7 cells and LPS-treated mice [36].”

    From Beneficial Effects of Spirulina Consumption on Brain Health (2022) · cites this paper as [36]

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Authors
Katsuura, S., Imamura, T., Bando, N., & Yamanishi, R.
Affiliations
Tokushima University (JP)
Corresponding author
Yamanishi, Rintaro
Venue
Molecular Nutrition & Food Research
Publisher
Wiley
Published
2009-11
Volume
53
Issue
11
Pages
1396-1405
ISSN
  • 1613-4125 (ISSN-L)
  • 1613-4133
OpenAlex
W2047950591
Cited by
76
References
41
Citations by year
2026: 1 · 2025: 5 · 2024: 1 · 2023: 5 · 2022: 5 · 2021: 9 · 2020: 4 · 2019: 8 · 2018: 5 · 2017: 5 · 2016: 6 · 2015: 3 · 2014: 3 · 2013: 8 · 2012: 4
FWCI
1.72
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular Nutrition & Food Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
Keywords
beta-carotene, beta-cryptoxanthin, lutein, carotenoids, macrophages, RAW264 cells, lipid peroxidation, glutathione, redox state, inflammatory cytokines, IL-1β, interleukin-6, IL-12p40, carotenoid accumulation, immune regulation
MeSH terms
Animals, Cells, Cultured, Glutathione, Macrophages, Oxidation-Reduction, Lutein, Lipid Peroxidation, beta Carotene, Glutathione Disulfide, Xanthophylls, Mice, Cryptoxanthins

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