Withanamides in Withania somnifera fruit protect PC‐12 cells from β‐amyloid responsible for Alzheimer's disease
Open accessAbstract
Alzheimer's disease (AD) is an irreversible neurodegenerative disorder with symptoms of confusion, memory loss, and mood swings. The beta-amyloid peptide, with 39-42 amino acid residues (BAP), plays a significant role in the development of AD. Although there is no cure for AD, it can be managed with available drugs to some degree. Several studies have revealed that natural antioxidants, such as vitamin E, vitamin C and beta-carotene, may help in scavenging free radicals generated during the initiation and progression of this disease. Therefore, there has been considerable interest in plant phytochemicals with antioxidant property as potential agents to prevent the progression of AD. Our earlier investigations of the Withania somnifera fruit afforded lipid peroxidation inhibitory withanamides that are more potent than the commercial antioxidants. In this study, we have tested two major withanamides A (WA) and C (WC) for their ability to protect the PC-12 cells, rat neuronal cells, from beta-amyloid induced cell damage. The cell death caused by beta-amyloid was negated by withanamide treatment. Molecular modeling studies showed that withanamides A and C uniquely bind to the active motif of beta-amyloid (25-35) and suggest that withanamides have the ability to prevent the fibril formation. Further understanding of the mechanism of action and in vivo efficacy of these withanamides may facilitate its development as a prophylaxis.
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Withanamides (A/C) present in W. somnifera fruits protect pheochromocytoma-(PC-12) from β-amyloid induced toxicity.
“A study carried out by Jayaprakasam et al. [160] stated that withanamides (A/C) present in W. somnifera fruits protect pheochromocytoma-(PC-12) from β-amyloid induced toxicity.”
From Indian Medicinal Herbs and Formulations for Alzheimer’s Disease, from Traditional Knowledge to Scientific Assessment (2020) · cites this paper as [160]
Withanamides A and C protect neuronal rat cells and PC-12 cells from beta-amyloid-induced neuronal death.
“Subsequently, these compounds have been shown to protect neuronal rat cells and PC-12 cells from Beta-amyloid-induced neuronal death [51,86].”
From The Multifaceted Role of Neuroprotective Plants in Alzheimer’s Disease Treatment (2022) · cites this paper as [51]
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“Subsequently, these compounds have been shown to protect neuronal rat cells and PC-12 cells from Betaamyloid-induced neuronal death [51,86].”
Background
- Authors
- Jayaprakasam, B., Padmanabhan, K., & Nair, M. G.
- Affiliations
- Michigan State University (US)
- Corresponding author
- Nair, Muraleedharan G.
- Venue
- Phytotherapy Research
- Publisher
- Wiley
- Published
- 2010-05-26
- Volume
- 24
- Issue
- 6
- Pages
- 859-863
- ISSN
- 0951-418X (ISSN-L)
- 1099-1573
- OpenAlex
- W2020660605
- Cited by
- 142
- References
- 21
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 9 · 2023: 16 · 2022: 11 · 2021: 15 · 2020: 16 · 2019: 3 · 2018: 7 · 2017: 11 · 2016: 8 · 2015: 7 · 2014: 5 · 2013: 8 · 2012: 6
- FWCI
- 9.78
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Phytotherapy Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Michigan State University
- College of Engineering, Michigan State University
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Keywords
- Withania somnifera, amyloid-beta, Alzheimer's disease, PC12 cells, neuroprotection, lipid peroxidation inhibition, antioxidant phytochemicals, amyloid fibril formation, molecular docking, neurodegeneration
- MeSH terms
- Alzheimer Disease, Amides, Animals, Cell Survival, Disaccharides, Fruit, Indoles, Models, Molecular, Peptide Fragments, Molecular Structure, Amyloid beta-Peptides, PC12 Cells, Withania, Rats
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