An Aqueous Extract of Withania somnifera Root Inhibits Amyloid β Fibril Formation In Vitro
Abstract
The ability of an aqueous extract of W. somnifera L. Dunal (Family: Solanaceae) roots to inhibit fibril formation by the amyloid-β peptide in vitro was investigated. W. somnifera is used extensively in traditional Ayurvedic medicine as a nerve tonic with reputed memory enhancing properties. Inhibition of fibrillogenesis measured by transmission electron microscopy and ThT fluorescence assay showed that an aqueous extract of W. somnifera strongly inhibited Aβ fibril formation in a concentration-dependent manner, when compared with control samples. These results suggest that the aqueous extract of W. somnifera root has an ability to inhibit the formation of mature amyloid-β fibrils in vitro, which are known to lead to amyloid plaque formation in vivo.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
An in-vitro inhibitory effect on the fibril formation by Aβ peptide has been reported for Withania.
“An in-vitro, inhibitory effect on the fibril formation by Aβ peptide has also been reported [163].”
From Indian Medicinal Herbs and Formulations for Alzheimer’s Disease, from Traditional Knowledge to Scientific Assessment (2020) · cites this paper as [163]
Cites
1 retracted
Retracted:Nootropic‐like effect of ashwagandha (Withania somnifera L.) in mice
Dhuley, J. N. (2001) · Phytotherapy Research · doi:10.1002/ptr.874 · Cited by 85 · see citing works
RetractedGlenner, G. G., & Wong, C. W. (1984) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(84)80190-4 · Cited by 5,404 · see citing works
Amyloid plaque core protein in Alzheimer disease and Down syndrome.
Masters, C. L., Simms, G., Weinman, N. A., Multhaup, G., McDonald, B. L., & Beyreuther, K. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.12.4245 · Cited by 4,431 · see citing works
Alzheimer's Disease Is a Synaptic Failure
Selkoe, D. J. (2002) · Science · doi:10.1126/science.1074069 · Cited by 4,420 · see citing works
[18] Quantification of β-sheet amyloid fibril structures with thioflavin T
LeVine, H. (1999) · Methods in Enzymology · doi:10.1016/s0076-6879(99)09020-5 · Cited by 1,365 · cited 1× · see citing works
“Aβ fibrillogenesis (peptide aggregation) was measured by a ThT assay, in which the fluorescence intensity reflects the degree of aggregation (LeVine, 1999).”
MethodBeta-amyloid neurotoxicity requires fibril formation and is inhibited by congo red.
Lorenzo, A., & Yankner, B. A. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.25.12243 · Cited by 1,324 · see citing works
Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye, thioflavine T
Naiki, H., Higuchi, K., Hosokawa, M., & Takeda, T. (1989) · Analytical Biochemistry · doi:10.1016/0003-2697(89)90046-8 · Cited by 1,224 · see citing works
Porat, Y., Abramowitz, A., & Gazit, E. (2005) · Chemical Biology & Drug Design · doi:10.1111/j.1747-0285.2005.00318.x · Cited by 994 · see citing works
Bhattacharya, A., Ghosal, S., & Bhattacharya, S. K. (2001) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(00)00309-3 · Cited by 288 · see citing works
Antioxidant activity of glycowithanolides from Withania somnifera.
Bhattacharya, S. K., Satyan, K. S., & Ghosal, S. (1997) · PubMed · Cited by 287 · see citing works
Bates, K. A., Verdile, G., Li, Q.-X., Ames, D., Hudson, P., Masters, C. L., & Martins, R. N. (2009) · Molecular Psychiatry · doi:10.1038/mp.2008.96 · Cited by 247 · see citing works
Neuroprotective effects of Withania somnifera on 6-hydroxydopamine induced Parkinsonism in rats
Ahmad, M., Saleem, S., Ahmad, A. S., Ansari, M. A., Yousuf, S., Hoda, M. N., & Islam, F. (2005) · Human & Experimental Toxicology · doi:10.1191/0960327105ht509oa · Cited by 227 · see citing works
Ayurvedic medicinal plants for Alzheimer’s disease: A review
Rao, R. V., Descamps, O., John, V., & Bredesen, D. E. (2012) · Alzheimer's Research & Therapy · doi:10.1186/alzrt125 · Cited by 213 · see citing works
Withanoside IV and its active metabolite, sominone, attenuate Aβ(25–35)‐induced neurodegeneration
Kuboyama, T., Tohda, C., & Komatsu, K. (2006) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2006.04664.x · Cited by 171 · see citing works
Jayaprakasam, B., Padmanabhan, K., & Nair, M. G. (2010) · Phytotherapy Research · doi:10.1002/ptr.3033 · Cited by 142 · see citing works
MARTINEZ, C., Albet, C., Agúndez, J. A. G., HERRERO, E., Carrillo, J. A., MARQUEZ, M., Benítez, J., & ORTIZ, J. (1999) · Clinical Pharmacology & Therapeutics · doi:10.1016/s0009-9236(99)70129-3 · Cited by 132 · see citing works
Parihar, M. S., Chaudhary, M., Shetty, R., & Hemnani, T. (2004) · Journal of Clinical Neuroscience · doi:10.1016/j.jocn.2003.09.008 · Cited by 121 · see citing works
Choudhary, M. I., Nawaz, S. A., Ul‐Haq, Z., Azim, M. K., Ghayur, M. N., Lodhi, M. A., Jalil, S., Khalid, A., Ahmed, A., Rode, B. M., et al. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.05.068 · Cited by 102 · see citing works
Jain, S., Shukla, S. D., Sharma, K., & Bhatnagar, M. (2001) · Phytotherapy Research · doi:10.1002/ptr.802 · Cited by 101 · see citing works
Kumar, S., Seal, C. J., Howes, M. R., Kite, G. C., & Okello, E. J. (2010) · Phytotherapy Research · doi:10.1002/ptr.3261 · Cited by 84 · see citing works
Self-citationNaidu, P. S., Singh, A., & Kulkarni, S. K. (2006) · Phytotherapy Research · doi:10.1002/ptr.1823 · Cited by 77 · see citing works
Neurodegenerative mechanisms in alzheimer disease
Sopher, B. L., Fukuchi, K., Kavanagh, T. J., Furlong, C. E., & Martin, G. M. (1996) · Molecular and Chemical Neuropathology · doi:10.1007/bf02814999 · Cited by 75 · see citing works
Negative Staining of Thinly Spread Biological Samples
Harris, J. R. (2007) · Methods in molecular biology · doi:10.1007/978-1-59745-294-6_7 · Cited by 70 · see citing works
In Vivo and In Vitro Anti-inflammatory Activities of Neoandrographolide
Liu, J., Wang, Z., & Ji, L. (2007) · The American Journal of Chinese Medicine · doi:10.1142/s0192415x07004849 · Cited by 68 · see citing works
Harris, J. R. (2002) · Micron · doi:10.1016/s0968-4328(02)00029-x · Cited by 59 · see citing works
Hucul, J. A., Henshaw, E. C., & Young, D. A. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36298-1 · Cited by 49 · see citing works
Cholesterol binding to amyloid-β fibrils: A TEM study
Harris, J. R. (2008) · Micron · doi:10.1016/j.micron.2008.05.001 · Cited by 35 · see citing works
The Amyloid Hypothesis and the clearance and degradation of Alzheimer's β-peptide
LeVine, H. (2004) · Journal of Alzheimer s Disease · doi:10.3233/jad-2004-6311 · Cited by 20 · see citing works
Alberts, D. S., Dorr, R. T., Wunz, T. P., et al. (1991) · Anti-Cancer Drugs · doi:10.1097/00001813-199102000-00010 · Cited by 2 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Ayurvedic medicinal plants for Alzheimer’s disease: A review
Rao, R. V., Descamps, O., John, V., & Bredesen, D. E. (2012) · Alzheimer's Research & Therapy · doi:10.1186/alzrt125 · Cited by 213 · see citing works
Langade, D., Kanchi, S., Salve, J., Debnath, K., & Ambegaokar, D. (2019) · Cureus · doi:10.7759/cureus.5797 · Cited by 129 · see citing works
Mehla, J., Gupta, P., Pahuja, M., Diwan, D., & Diksha, D. (2020) · Brain Sciences · doi:10.3390/brainsci10120964 · Cited by 108 · see citing works
Improving the inhibition of β-amyloid aggregation by withanolide and withanoside derivatives
Dubey, S., Kallubai, M., & Subramanyam, R. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.01.094 · Cited by 29 · see citing works
The Multifaceted Role of Neuroprotective Plants in Alzheimer’s Disease Treatment
Zieneldien, T., Kim, J., & Cao, C. (2022) · Geriatrics · doi:10.3390/geriatrics7020024 · Cited by 29 · see citing works
“search studies have elucidated that withanamides A and C have the ability to bind to the active motif of Abeta-25-35 and avert the formation of fibrils [85].”
Background
- Authors
- Kumar, S., Harris, R. J., Seal, C. J., & Okello, E. J.
- Affiliations
- Guru Gobind Singh Indraprastha University (IN)
- Johannes Gutenberg University Mainz (DE)
- Newcastle University (GB)
- Corresponding author
- Okello, Edward J.
- Venue
- Phytotherapy Research
- Publisher
- Wiley
- Published
- 2011-05-12
- Volume
- 26
- Issue
- 1
- Pages
- 113-117
- ISSN
- 0951-418X (ISSN-L)
- 1099-1573
- OpenAlex
- W2112486905
- Cited by
- 62
- References
- 29
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 4 · 2023: 4 · 2022: 7 · 2021: 8 · 2020: 7 · 2019: 6 · 2018: 3 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 4 · 2013: 3 · 2012: 3
- FWCI
- 8.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Phytotherapy Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Keywords
- Withania somnifera, ashwagandha root, aqueous plant extract, amyloid-beta, fibril formation inhibition, amyloid plaques, Alzheimer's disease, neuroprotection, Ayurveda
- MeSH terms
- Amyloid, Cells, Cultured, Dose-Response Relationship, Drug, Fluorescence, Medicine, Ayurvedic, Phytotherapy, Plant Extracts, Amyloid beta-Peptides, Plant Roots, Withania, Microscopy, Electron, Transmission, Plaque, Amyloid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review