Antipyretic and Anti-Inflammatory Effects of Asiaticoside in Lipopolysaccharide-Treated Rat through up-Regulation of Heme Oxygenase-1
Abstract
Asiaticoside (AS), a triterpenoid isolated from Centella asiatica, has been found to exhibit antioxidant and anti-inflammatory activities in several experimental animal models. However, the underlying mechanisms remain elusive. In this study, we provide experimental evidences that AS dose-dependently inhibited lipopolysaccharide (LPS)-induced fever and inflammatory response, including serum tumor necrosis factor (TNF)-α and interleukin (IL)-6 production, liver myeloperoxidase (MPO) activity, brain cyclooxygenase-2 (COX-2) protein expression and prostaglandin E2 (PGE2 ) production. Interestingly, AS increased serum IL-10 level, liver heme oxygenase-1 (HO-1) protein expression and activity. Furthermore, we found that the suppressive effects of AS on LPS-induced fever and inflammation were reversed by pretreatment with ZnPPIX, a HO-1 activity inhibitor. In summary, our results suggest that AS has the antipyretic and anti-inflammatory effects in LPS-treated rat. These effects could be associated with the inhibition of pro-inflammatory mediators, including TNF-α and IL-6 levels, COX-2 expression and PGE2 production, as well as MPO activity, which might be mediated by the up-regulation of HO-1.
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C. asiatica has an anti-inflammatory effect observed by reduction in IL-1β, IL-6, TNFα, PGE2, and COX-2.
“C. asiatica has shown an anti-inflammatory effect observed by the reduction in Interleukin-1β (IL-1β), Interleukin-6 (IL-6) and Tumour Necrosis Factor α (TNFα) [33,36,37], as well as prostaglandin E2 (PGE2) [36,38], and cyclooxygenase-2 (COX-2) [39].”
From A systematic review of the effect of Centella asiatica on wound healing (2022) · cites this paper as [39]
This enhancement might result from the anti-inflammatory effect of C. asiatica due to reduction in IL-1β, IL-6, TNFα, PGE2, and COX-2.
“This might result from the anti-inflammatory effect caused by C. asiatica due to the reduction in IL-1β, IL-6 and TNFα [33,36,37], as well as prostaglandin E2 (PGE2) [36,38] and cyclooxygenase-2 (COX-2) [39].”
From A systematic review of the effect of Centella asiatica on wound healing (2022) · cites this paper as [39]
Asiaticoside dose-dependently suppresses LPS-induced fever and inflammation in rats, presumably by inhibition of pro-inflammatory mediators (serum TNF-α, IL-6, liver MPO, brain COX-2) and upregulation of liver HO-1 protein activity.
“asiaticoside acts as an anti‐inflammatory and antipyretic agent that has been studied to dose‐dependently suppress lipopolysaccharide (LPS)‐induced fever and inflammation in rats, presumably by the inhibition of pro‐inflammatory mediators (serum tumour necrosis factor (TNF)‐α, interleukin (IL)‐6, liver myeloperoxidase (MPO), brain cyclooxygenase‐2 (COX‐2)) and the upregulation of liver heme oxygen”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [143]
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- Authors
- Wan, J., Gong, X., Jiang, R., Zhang, Z., & Zhang, L.
- Affiliations
- Chongqing Medical University (CN)
- Corresponding author
- Zhang, Li
- Venue
- Phytotherapy Research
- Publisher
- Wiley
- Published
- 2012-09-12
- Volume
- 27
- Issue
- 8
- Pages
- 1136-1142
- ISSN
- 0951-418X (ISSN-L)
- 1099-1573
- OpenAlex
- W1598910025
- Cited by
- 75
- References
- 36
- Citations by year
- 2026: 1 · 2025: 8 · 2024: 7 · 2023: 5 · 2022: 6 · 2021: 10 · 2020: 6 · 2019: 6 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 6 · 2014: 11 · 2013: 1 · 2012: 1
- FWCI
- 2.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Phytotherapy Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heme Oxygenase-1 and Carbon Monoxide
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
- Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
- Keywords
- asiaticoside, Centella asiatica, heme oxygenase-1, lipopolysaccharide-induced fever, anti-inflammatory activity, antipyretic activity, TNF-α, interleukin-6, IL-10, cyclooxygenase-2, prostaglandin E2, myeloperoxidase, triterpenoids
- MeSH terms
- Animals, Anti-Inflammatory Agents, Heme Oxygenase (Decyclizing), Inflammation, Lipopolysaccharides, Liver, Male, Peroxidase, Triterpenes, Tumor Necrosis Factor-alpha, Dinoprostone, Interleukin-6, Up-Regulation, Interleukin-10, Rats, Sprague-Dawley, Centella, Rats, Cyclooxygenase 2, Antipyretics
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