Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 171

The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.

Physiological Levels of Reactive Oxygen Species Are Required to Maintain Genomic Stability in Stem Cells

Open access
Li, Tao-Sheng, Marbán, Eduardo·2010·Stem Cells
DOI 10.1002/stem.438
Open this paper in CoChat — free account

Abstract

Stem cell cytogenetic abnormalities constitute a roadblock to regenerative therapies. We investigated the possibility that reactive oxygen species (ROSs) influence genomic stability in cardiac and embryonic stem cells. Karyotypic abnormalities in primary human cardiac stem cells were suppressed by culture in physiological (5%) oxygen, but addition of antioxidants to the medium unexpectedly increased aneuploidy. Intracellular ROS levels were moderately decreased in physiological oxygen, but dramatically decreased by the addition of high-dose antioxidants. Quantification of DNA damage in cardiac stem cells and in human embryonic stem cells revealed a biphasic dose-dependence: antioxidants suppressed DNA damage at low concentrations, but potentiated such damage at higher concentrations. High-dose antioxidants decreased cellular levels of ATM (ataxia-telangiectasia mutated) and other DNA repair enzymes, providing a potential mechanistic basis for the observed effects. These results indicate that physiological levels of intracellular ROS are required to activate the DNA repair pathway for maintaining genomic stability in stem cells. The concept of an “oxidative optimum” for genomic stability has broad implications for stem cell biology and carcinogenesis.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1002/stem.438

Cites

6 of 6 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Research works printed but not linked: 29

  1. Maitra, 2005, Genomic alterations in cultured human embryonic stem cells, Nat Genet, 37, 1099, 10.1038/ng1631 · DOI · Paper page
  2. Baker, 2007, Adaptation to culture of human embryonic stem cells and oncogenesis in vivo, Nat Biotechnol, 25, 207, 10.1038/nbt1285 · DOI · Paper page
  3. Smith, 2007, Regenerative potential of cardiosphere-derived cells expanded from percutaneous endomyocardial biopsy specimens, Circulation, 115, 896, 10.1161/CIRCULATIONAHA.106.655209 · DOI · Paper page
  4. Rubio, 2005, Spontaneous human adult stem cell transformation, Cancer Res, 65, 3035, 10.1158/0008-5472.CAN-04-4194 · DOI · Paper page
  5. Furlani, 2009, A transformed cell population derived from cultured mesenchymal stem cells has no functional effect after transplantation into the injured heart, Cell Transplant, 18, 319, 10.3727/096368909788534906 · DOI
  6. Ivanovic, 2009, Hypoxia or in situ normoxia: The stem cell paradigm, J Cell Physiol, 219, 271, 10.1002/jcp.21690 · DOI · Paper page
  7. Ames, 1993, Oxidants, antioxidants, and the degenerative diseases of aging, Proc Natl Acad Sci USA, 90, 7915, 10.1073/pnas.90.17.7915 · DOI · Paper page
  8. Shaffer, 2009, An International System for Human Cytogenetic Nomenclature
  9. Stewart, 2005, Revision of the 1990 working formulation for the standardization of nomenclature in the diagnosis of heart rejection, J Heart Lung Transplant, 24, 1710, 10.1016/j.healun.2005.03.019 · DOI · Paper page
  10. Lavon, 2008, Derivation of euploid human embryonic stem cells from aneuploid embryos, Stem Cells, 26, 1874, 10.1634/stemcells.2008-0156 · DOI
  11. Lobo, 2008, Chromosome abnormalities and cancer cytogenetics, Nature Educ, 1
  12. Wang, 2006, Establishment of new mouse embryonic stem cell lines is improved by physiological glucose and oxygen, Cloning Stem Cells, 8, 108, 10.1089/clo.2006.8.108 · DOI
  13. Fehrer, 2007, Reduced oxygen tension attenuates differentiation capacity of human mesenchymal stem cells and prolongs their lifespan, Aging Cell, 6, 745, 10.1111/j.1474-9726.2007.00336.x · DOI · Paper page
  14. Hainsworth, 2008, The nitrone disodium 2,4-sulphophenyl-N-tert-butylnitrone is without cytoprotective effect on sodium nitroprusside-induced cell death in N1E-115 neuroblastoma cells in vitro, J Cereb Blood Flow Metab, 28, 24, 10.1038/sj.jcbfm.9600517 · DOI
  15. Qin, 2009, ATM-mediated transcriptional elevation of prion in response to copper-induced oxidative stress, J Biol Chem, 284, 4582, 10.1074/jbc.M808410200 · DOI
  16. Falck, 2005, Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage, Nature, 434, 605, 10.1038/nature03442 · DOI · Paper page
  17. Bredemeyer, 2006, ATM stabilizes DNA double-strand-break complexes during V(D)J recombination, Nature, 442, 466, 10.1038/nature04866 · DOI · Paper page
  18. Bao, 2001, ATR/ATM-mediated phosphorylation of human Rad17 is required for genotoxic stress responses, Nature, 411, 969, 10.1038/35082110 · DOI · Paper page
  19. Seifried, 2007, A review of the interaction among dietary antioxidants and reactive oxygen species, J Nutr Biochem, 18, 567, 10.1016/j.jnutbio.2006.10.007 · DOI · Paper page
  20. Sareen, 2009, Chromosome 7 and 19 trisomy in cultured human neural progenitor cells, Plos One, 4, e7630, 10.1371/journal.pone.0007630 · DOI
  21. Djokic, 2006, Post-transplant lymphoproliferative disorder subtypes correlate with different recurring chromosomal abnormalities, Genes Chromosomes Cancer, 45, 313, 10.1002/gcc.20287 · doi:10.1002/gcc.20287
  22. Sesso, 2008, Vitamins E and C in the prevention of cardiovascular disease in men: The Physicians' Health Study II randomized controlled trial, JAMA, 300, 2123, 10.1001/jama.2008.600 · DOI · Paper page
  23. Miller, 2005, Meta-analysis: High-dosage vitamin E supplementation may increase all-cause mortality, Ann Intern Med, 142, 37, 10.7326/0003-4819-142-1-200501040-00110 · DOI · Paper page
  24. Foreman, 2003, Reactive oxygen species produced by NADPH oxidase regulate plant cell growth, Nature, 422, 442, 10.1038/nature01485 · DOI · Paper page
  25. Niethammer, 2009, A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish, Nature, 459, 996, 10.1038/nature08119 · DOI · Paper page
  26. Owusu-Ansah, 2009, Reactive oxygen species prime Drosophila haematopoietic progenitors for differentiation, Nature, 461, 537, 10.1038/nature08313 · DOI · Paper page
  27. Chen, 2009, Reduced tumorigenesis in p53 knockout mice exposed in utero to low-dose vitamin E, Cancer, 115, 1563, 10.1002/cncr.24130 · DOI
  28. Chen, 2006, Enhanced tumorigenesis in p53 knockout mice exposed in utero to high-dose vitamin E, Carcinogenesis, 27, 1358, 10.1093/carcin/bgi325 · DOI
  29. Singh, 2007, High-dose alpha-tocopherol therapy does not affect HDL subfractions in patients with coronary artery disease on statin therapy, Clin Chem, 53, 525, 10.1373/clinchem.2006.078865 · DOI

Cited by

See all 171

Metadata

Authors
Li, T.-S., & Marbán, E.
Venue
Stem Cells
Publisher
Oxford University Press (OUP)
Published
2010-05-04
Volume
28
Issue
7
Pages
1178-1185
ISSN
  • 1066-5099 (ISSN-L)
  • 1549-4918
DOI
10.1002/stem.438
OpenAlex
W2084690414
Cited by
171
References
6
FWCI
5.79
Type
journal article
Language
en
Open access
green
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Funders
NIH (R01HL083109)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.