Cited by 147RNA Research and SplicingRNA regulation and diseaseRNA and protein synthesis mechanisms

Pre‐mRNA 3′‐end processing complex assembly and function

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Chan, Serena, Choi, Eun‐A, Shi, Yongsheng·2011·WIREs RNA

Abstract

The 3′‐ends of almost all eukaryotic mRNAs are formed in a two‐step process, an endonucleolytic cleavage followed by polyadenylation (the addition of a poly‐adenosine or poly(A) tail). These reactions take place in the pre‐mRNA 3′ processing complex, a macromolecular machinery that consists of more than 20 proteins. A general framework for how the pre‐mRNA 3′ processing complex assembles and functions has emerged from extensive studies over the past several decades using biochemical, genetic, computational, and structural approaches. In this article, we review what we have learned about this important cellular machine and discuss the remaining questions and future challenges. WIREs RNA 2011 2 321–335 DOI: 10.1002/wrna.54 This article is categorized under: RNA Processing > 3' End Processing

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  • IPA1 shares many genetic interactions in common with genes encoding members of the CPF and CF IA protein complexes.

    “IPA1 shares many genetic interactions in common with genes encoding members of the Cleavage/Polyadenylation factor (CPF) and Cleavage Factor IA (CF IA) protein complexes (Fig. 4B, C), which along with HRP1, are essential for mRNA 3′ end processing (19).”

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Authors
Chan, S., Choi, E., & Shi, Y.
Affiliations
University of California, Irvine (US)
Corresponding author
Shi, Yongsheng
Venue
WIREs RNA
Publisher
Wiley
Published
2010-10-18
Volume
2
Issue
3
Pages
321-335
ISSN
  • 1757-7004 (ISSN-L)
  • 1757-7012
OpenAlex
W1984594593
Cited by
147
References
117
Citations by year
2026: 4 · 2025: 7 · 2024: 11 · 2023: 7 · 2022: 7 · 2021: 13 · 2020: 10 · 2019: 8 · 2018: 8 · 2017: 12 · 2016: 9 · 2015: 5 · 2014: 20 · 2013: 14 · 2012: 11
FWCI
0.71
Type
journal article
Language
en
Open access
Locations
  • Wiley Interdisciplinary Reviews - RNA (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of General Medical Sciences (R01GM090056)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
pre-mRNA 3' end processing, polyadenylation, endonucleolytic cleavage, poly(A) tail, cleavage and polyadenylation specificity factor, CPSF6, poly(A) polymerase, protein complex formation, mRNA biogenesis
MeSH terms
Animals, Humans, Models, Biological, Plants, Polynucleotide Adenylyltransferase, DNA-Directed RNA Polymerases, RNA Precursors, Saccharomyces cerevisiae, RNA-Binding Proteins, RNA 3' End Processing, Poly(A)-Binding Proteins, RNA 3' Polyadenylation Signals, mRNA Cleavage and Polyadenylation Factors, Macromolecular Substances, Protein Interaction Domains and Motifs

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