Effects of pH, Nitrite, and Ascorbic Acid on Nonenzymatic Nitric Oxide Generation and Bacterial Growth in Urine
Abstract
Nitrite may be generated by bacteria in urine during urinary tract infections. Acidification of nitrite results in the formation of nitric oxide (NO) and other reactive nitrogen oxides, which are toxic to a variety of microorganisms. We have studied NO formation and bacterial growth in mildly acidified human urine containing nitrite and the reducing agent vitamin C. Urine collected from healthy subjects was incubated in closed syringes at different pH values with varying amounts of nitrite and/or ascorbic acid added. NO generation was measured in headspace gas using a chemiluminescence technique. A similar setup was also used to study the growth of three strains of bacteria in urine. Mildly acidified nitrite-containing urine generated large amounts of NO and this production was greatly potentiated by ascorbic acid. The growth of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus saprophyticus was markedly reduced by the addition of nitrite to acidified urine. This inhibition was enhanced by ascorbic acid. In conclusion, we show that the growth of three common urinary pathogens is markedly inhibited in mildly acidified urine when nitrite is present. The bacteriostatic effect of acidified nitrite is likely related to the release of NO and other toxic reactive nitrogen intermediates. These results may help to explain the well-known beneficial effects of urinary acidification with, e.g., vitamin C in treatment and prevention of urinary tract infection.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Supplementation of drinking water with nitrite enhanced graft survival to more than 120 days compared with 50 days in control animals on a normal diet.
“Supplementation of drinking water with nitrite enhanced graft survival to more than 120 days compared with 50 days in control animals on a normal diet.”
From Nitrate-Nitrite-Nitric Oxide Pathway: Implications for Anesthesiology and Intensive Care (2010) · cites this paper as [163]
In animals on a low nitrate/nitrite diet, allograft survival was significantly reduced to 31 days.
“In contrast, in animals on a low nitrate/nitrite diet, allograft survival was significantly reduced to 31 days.”
From Nitrate-Nitrite-Nitric Oxide Pathway: Implications for Anesthesiology and Intensive Care (2010) · cites this paper as [163]
These differences were accompanied by amelioration of histopathologic changes in the allografts as well as decreased tissue messenger RNA concentrations of interferon-γ and tumor necrosis factor-α.
“These differences were accompanied by amelioration of histopathologic changes in the allografts as well as in decreased tissue messenger RNA concentrations of interferon-␥ and tumor necrosis factor-␣.”
From Nitrate-Nitrite-Nitric Oxide Pathway: Implications for Anesthesiology and Intensive Care (2010) · cites this paper as [163]
Cites
Nitric oxide synthases in mammals
Knowles, R. G., & Moncada, S. (1994) · Biochemical Journal · doi:10.1042/bj2980249 · Cited by 2,752 · see citing works
Intragastric nitric oxide production in humans: measurements in expelled air.
Lundberg, J. O., Weitzberg, E., Lundberg, J. M., & Alving, K. (1994) · Gut · doi:10.1136/gut.35.11.1543 · Cited by 661 · see citing works
Self-citationChemical synthesis of nitric oxide in the stomach from dietary nitrate in humans
McKnight, G. M., Smith, L. M., Drummond, R. S., Duncan, C. W., Golden, M., & Benjamin, N. (1997) · Gut · doi:10.1136/gut.40.2.211 · Cited by 375 · see citing works
NO Inhibitions: Antimicrobial Properties of Nitric Oxide
Groote, M. A. D., & Fang, F. C. (1995) · Clinical Infectious Diseases · doi:10.1093/clinids/21.supplement_2.s162 · Cited by 353 · see citing works
Inhibitors of endogenous nitrosation mechanisms and implications in human cancer prevention
Bartsch, H., Ohshima, H., & Pignatelli, B. (1988) · Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis · doi:10.1016/0027-5107(88)90194-7 · Cited by 296 · see citing works
Reactive nitrogen intermediates and antimicrobial activity: Role of nitrite
Klebanoff, S. J. (1993) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(93)90084-8 · Cited by 192 · see citing works
Licht, W. R., Tannenbaum, S. R., & Deen, W. M. (1988) · Carcinogenesis · doi:10.1093/carcin/9.3.365 · Cited by 129 · see citing works
Effect of nitric oxide on staphylococcal killing and interactive effect with superoxide
Kaplan, S. S., Lancaster, J. R., BASFORD, R. E., & Simmons, R. L. (1996) · Infection and Immunity · doi:10.1128/iai.64.1.69-76.1996 · Cited by 119 · see citing works
Urinary nitrite: more than a marker of infection
Lundberg, J. O. N., Carlsson, S., Engstrand, L., Morcos, E., Wiklund, N. P., & Weitzberg, E. (1997) · Urology · doi:10.1016/s0090-4295(97)00257-4 · Cited by 108 · see citing works
Self-citationAthmann, C., Zeng, N., Kang, T., Marcus, E. A., Scott, D. R., Rektorschek, M., Buhmann, A., Melchers, K., & Sachs, G. M. (2000) · Journal of Clinical Investigation · doi:10.1172/jci9351 · Cited by 68 · see citing works
The Biochemical and Serological Relationships of the Organisms of the GenusProteus
Rustigian, R., & Stuart, C. A. (1945) · Journal of Bacteriology · doi:10.1128/jb.49.5.419-436.1945 · Cited by 57 · see citing works
Prevention of Recurrent Urinary Tract Infections in Girls
Holland, N. H. (1963) · Archives of Pediatrics and Adolescent Medicine · doi:10.1001/archpedi.1963.02080040562006 · Cited by 33 · see citing works
Effect of ascorbic acid on Urine pH in Man
Nahata, M. C., Shrimp, L., Lampman, T., & McLeod, D. C. (1977) · American Journal of Health-System Pharmacy · doi:10.1093/ajhp/34.11.1234 · Cited by 32 · see citing works
McDonald, D. F., & Murphy, G. P. (1959) · New England Journal of Medicine · doi:10.1056/nejm195910152611608 · Cited by 30 · see citing works
Urinary acidification with ascorbic acid
Travis, L. B., Dodge, W. F., Mintz, A. A., & Assemi, M. (1965) · The Journal of Pediatrics · doi:10.1016/s0022-3476(65)80223-2 · Cited by 27 · see citing works
The Effect of pH on the Growth of Bacteria in Urine
Yeaw, R. C. (1940) · The Journal of Urology · doi:10.1016/s0022-5347(17)71317-1 · Cited by 19 · see citing works
Ineffectiveness of Intravenous Ascorbic Acid as an Acidifying Agent in Man
Barton, C. H. (1981) · Archives of Internal Medicine · doi:10.1001/archinte.141.2.211 · Cited by 17 · see citing works
Brandt, R., Guyer, K. E., & Banks, W. L. (1977) · American Journal of Clinical Pathology · doi:10.1093/ajcp/68.5.592 · Cited by 15 · see citing works
Ueber den bactericiden Einfluss der Acidität des Harns auf die Cystitiserreger
Rostoski, O. (1898) · DMW - Deutsche Medizinische Wochenschrift · doi:10.1055/s-0029-1204338 · Cited by 9 · see citing works
Urinary nitrite in Japanese patients with urinary tract infections
Mizutani, T., Akinaga, N., & Takatera, H. (1989) · The Science of The Total Environment · doi:10.1016/0048-9697(89)90071-5 · Cited by 6 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics
Lundberg, J. O., Weitzberg, E., & Gladwin, M. T. (2008) · Nature Reviews Drug Discovery · doi:10.1038/nrd2466 · Cited by 2,739 · see citing works
Self-citationNitrate, bacteria and human health
Lundberg, J. O., Weitzberg, E., Cole, J. A., & Benjamin, N. (2004) · Nature Reviews Microbiology · doi:10.1038/nrmicro929 · Cited by 739 · see citing works
Self-citationGovoni, M., Jansson, E. Å., Weitzberg, E., & Lundberg, J. O. (2008) · Nitric Oxide · doi:10.1016/j.niox.2008.08.003 · Cited by 585 · see citing works
Self-citationThe role of nitrite and free nitrous acid (FNA) in wastewater treatment plants
Zhou, Y., Oehmen, A., Lim, M., Vadivelu, V. M., & Ng, W. J. (2011) · Water Research · doi:10.1016/j.watres.2011.06.025 · Cited by 476 · see citing works
REGULATION OF VITAMIN C TRANSPORT
Wilson, J. X. (2005) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.25.050304.092647 · Cited by 376 · see citing works
Evaluation of bacterial nitrate reduction in the human oral cavity
Doel, J. J., Benjamin, N., Hector, M. P., Rogers, M., & Allaker, R. P. (2005) · European Journal of Oral Sciences · doi:10.1111/j.1600-0722.2004.00184.x · Cited by 362 · see citing works
A mammalian functional nitrate reductase that regulates nitrite and nitric oxide homeostasis
Jansson, E. Å., Huang, L., Malkey, R., Govoni, M., Nihlén, C., Olsson, A., Stensdotter, M., Petersson, J., Holm, L., Weitzberg, E., et al. (2008) · Nature Chemical Biology · doi:10.1038/nchembio.92 · Cited by 356 · see citing works
Self-citationRoles of dietary inorganic nitrate in cardiovascular health and disease
Lundberg, J. O., Carlström, M., Larsen, F. J., & Weitzberg, E. (2011) · Cardiovascular Research · doi:10.1093/cvr/cvq325 · Cited by 355 · see citing works
Self-citationNovel Aspects of Dietary Nitrate and Human Health
Weitzberg, E., & Lundberg, J. O. (2013) · Annual Review of Nutrition · doi:10.1146/annurev-nutr-071812-161159 · Cited by 324 · see citing works
Self-citationNitric Oxide: A Key Mediator of Biofilm Dispersal with Applications in Infectious Diseases
Barraud, N., Kelso, M. J., Rice, S., & Kjelleberg, S. J. (2014) · Current Pharmaceutical Design · doi:10.2174/1381612820666140905112822 · Cited by 255 · see citing works
Barbosa‐Cesnik, C., Brown, M. B., Buxton, M., Zhang, L., DeBusscher, J., & Foxman, B. (2010) · Clinical Infectious Diseases · doi:10.1093/cid/ciq073 · Cited by 250 · see citing works
Correcting a Fundamental Flaw in the Paradigm for Antimicrobial Susceptibility Testing
Ersoy, S. C., Heithoff, D. M., Barnes, L., V, Tripp, G. K., House, J. K., Marth, J. D., Smith, J. W., & Mahan, M. J. (2017) · EBioMedicine · doi:10.1016/j.ebiom.2017.05.026 · Cited by 213 · see citing works
Current Advances of Nitric Oxide in Cancer and Anticancer Therapeutics
Mintz, J. A., Vedenko, A., Rosete, O. J., Shah, K. H., Goldstein, G., Hare, J. M., Ramasamy, R., & Arora, H. (2021) · Vaccines · doi:10.3390/vaccines9020094 · Cited by 181 · see citing works
Therapeutic effects of inorganic nitrate and nitrite in cardiovascular and metabolic diseases
Omar, S. A., Webb, A. J., Lundberg, J. O., & Weitzberg, E. (2016) · Journal of Internal Medicine · doi:10.1111/joim.12441 · Cited by 175 · see citing works
Self-citationNO-synthase independent NO generation in mammals
Lundberg, J. O., & Weitzberg, E. (2010) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2010.02.136 · Cited by 172 · see citing works
Self-citationRecurrent urinary tract infections in women
Aydın, A., Ahmed, K., Zaman, I., Khan, M. S., & Prokar Dasgupta, A. (2014) · International Urogynecology Journal · doi:10.1007/s00192-014-2569-5 · Cited by 164 · see citing works
NO generation from inorganic nitrate and nitrite: Role in physiology, nutrition and therapeutics
Lundberg, J. O., & Weitzberg, E. (2009) · Archives of Pharmacal Research · doi:10.1007/s12272-009-1803-z · Cited by 153 · see citing works
Self-citationSymptoms, risk factors, diagnosis and treatment of urinary tract infections
Kaur, R., & Kaur, R. (2020) · Postgraduate Medical Journal · doi:10.1136/postgradmedj-2020-139090 · Cited by 150 · see citing works
Nitrate-Nitrite-Nitric Oxide Pathway: Implications for Anesthesiology and Intensive Care
Weitzberg, E., Hezel, M., Lundberg, J. O., & Warner, D. S. (2010) · Anesthesiology · doi:10.1097/aln.0b013e3181fcf3cc · Cited by 145 · see citing works
Self-citationNitric oxide accelerates seed germination in warm-season grasses
Sarath, G., Bethke, P. C., Jones, R. L., Baird, L. M., Hou, G., & Mitchell, R. B. (2005) · Planta · doi:10.1007/s00425-005-0162-3 · Cited by 144 · see citing works
Nitric oxide as a cellular antioxidant: A little goes a long way
Hummel, S. G., Fischer, A. J., Martin, S. M., Schafer, F. Q., & Buettner, G. R. (2005) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2005.08.047 · Cited by 143 · see citing works
Rocha, B. S., Gago, B., Barbosa, R. M., & Laranjinha, J. (2009) · Toxicology · doi:10.1016/j.tox.2009.09.008 · Cited by 142 · see citing works
Siripongpreda, T., Somchob, B., Rodthongkum, N., & Hoven, V. P. (2020) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2020.117506 · Cited by 130 · see citing works
Wawrysiuk, S., Naber, K. G., Rechberger, T., & Miotła, P. (2019) · Archives of Gynecology and Obstetrics · doi:10.1007/s00404-019-05256-z · Cited by 121 · see citing works
Generation of NO by probiotic bacteria in the gastrointestinal tract
Sobko, T., Huang, L., Midtvedt, T., Norin, E., Gustafsson, L. E., Norman, M., Jansson, E. Å., & Lundberg, J. O. (2006) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2006.06.020 · Cited by 118 · see citing works
Self-citationA comparison of organic and inorganic nitrates/nitrites
Omar, S. A., Artime, E., & Webb, A. J. (2012) · Nitric Oxide · doi:10.1016/j.niox.2012.03.008 · Cited by 106 · see citing works
Generation, Translocation, and Action of Nitric Oxide in Living Systems
Tennyson, A. G., & Lippard, S. J. (2011) · Chemistry & Biology · doi:10.1016/j.chembiol.2011.09.009 · Cited by 104 · see citing works
The Basics of Bacteriuria: Strategies of Microbes for Persistence in Urine
Ipe, D. S., Horton, E. M., & Ulett, G. C. (2016) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2016.00014 · Cited by 98 · see citing works
The nitrate‐nitrite‐nitric oxide pathway: Its role in human exercise physiology
Bailey, S. J., Vanhatalo, A., Winyard, P. G., & JONES, A. M. (2011) · European Journal of Sport Science · doi:10.1080/17461391.2011.635705 · Cited by 96 · see citing works
Lin, X. Y., Mao, Y., Li, P., Bai, Y., Chen, T., Wu, K., Chen, D., Yang, H., & Yang, L. (2021) · Advanced Science · doi:10.1002/advs.202004627 · Cited by 85 · see citing works
Regulation of mitochondrial function and energetics by reactive nitrogen oxides
Larsen, F. J., Schiffer, T. A., Weitzberg, E., & Lundberg, J. O. (2012) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2012.08.580 · Cited by 87 · see citing works
Self-citationDeep insights into urinary tract infections and effective natural remedies
Bazzaz, B. S. F., Fork, S. D., Ahmadi, R., & Khameneh, B. (2021) · African Journal of Urology · doi:10.1186/s12301-020-00111-z · Cited by 85 · see citing works
Effect of Blood Nitrite and Nitrate Levels on Murine Platelet Function
Park, J. W., Piknova, B., Huang, P. L., Noguchi, C. T., & Schechter, A. N. (2013) · PLoS ONE · doi:10.1371/journal.pone.0055699 · Cited by 82 · see citing works
Daily intake of 100 mg ascorbic acid as urinary tract infection prophylactic agent during pregnancy
Ochoa-Brust, G. J., Fernández, A. R., Villanueva-Ruiz, G. J., VELASCO, R., Trujillo-Hernández, B., & Vásquez, C. (2007) · Acta Obstetricia Et Gynecologica Scandinavica · doi:10.1080/00016340701273189 · Cited by 82 · see citing works
The good and bad of antioxidant foods: An immunological perspective
Gostner, J. M., Becker, K., Ueberall, F., & Fuchs, D. (2015) · Food and Chemical Toxicology · doi:10.1016/j.fct.2015.02.012 · Cited by 80 · see citing works
Host-dependent Induction of Transient Antibiotic Resistance: A Prelude to Treatment Failure
Kubicek-Sutherland, J. Z., Heithoff, D. M., Ersoy, S. C., Shimp, W. R., House, J. K., Marth, J. D., Smith, J. W., & Mahan, M. J. (2015) · EBioMedicine · doi:10.1016/j.ebiom.2015.08.012 · Cited by 68 · see citing works
Chirinos, J. A., & Zamani, P. L. (2016) · Current Heart Failure Reports · doi:10.1007/s11897-016-0277-9 · Cited by 65 · see citing works
S. Rocha, B., Gago, B., Pereira, C., M. Barbosa, R., Bartesaghi, S., O. Lundberg, J., Radi, R., & Laranjinha, J. (2011) · Current Drug Targets · doi:10.2174/138945011796150334 · Cited by 64 · see citing works
Self-citationPuebla-Barragan, S., Renaud, J. B., Sumarah, M. W., & Reid, G. (2020) · Scientific Reports · doi:10.1038/s41598-020-66662-x · Cited by 60 · see citing works
Parallel evolution of Nitric Oxide signaling: Diversity of synthesis and memory pathways
Moroz, L. L. (2011) · Frontiers in bioscience · doi:10.2741/3837 · Cited by 59 · see citing works
Intravesical Nitric Oxide Delivery for Prevention of Catheter-Associated Urinary Tract Infections
Carlsson, S., Weitzberg, E., Wiklund, P., & Lundberg, J. O. (2005) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.49.6.2352-2355.2005 · Cited by 59 · see citing works
Self-citationCarlsson, S., Govoni, M., Wiklund, P. N., Weitzberg, E., & Lundberg, J. O. (2003) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.47.12.3713-3718.2003 · Cited by 57 · see citing works
Self-citationAmaral, J. H., Montenegro, M. F., Pinheiro, L. C., Ferreira, G. C., Barroso, R. P., Costa-Filho, A. J., & Tanus-Santos, J. E. (2013) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2013.07.032 · Cited by 53 · see citing works
Hassan, H. A., Hafez, H. S., & Zeghebar, F. E. (2010) · Food and Chemical Toxicology · doi:10.1016/j.fct.2010.05.001 · Cited by 55 · see citing works
Svensson, L., Poljakovic, M., Säve, S., Gilberthorpe, N. J., Schön, T., Strid, S., Corker, H., Poole, R. K., & Persson, K. (2010) · Microbial Pathogenesis · doi:10.1016/j.micpath.2010.04.001 · Cited by 49 · see citing works
A voltammetric pH sensor for food and biological matrices
Vivaldi, F. M., Santalucia, D., Poma, N. V., Bonini, A., Salvo, P., Noce, L. D., Melai, B., Kirchhain, A., Kolivoška, V., Sokolová, R., et al. (2020) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2020.128650 · Cited by 49 · see citing works
Gago, B., Nyström, T., Cavaleiro, C., Rocha, B. S., Barbosa, R. M., Laranjinha, J., & Lundberg, J. O. (2008) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2008.04.027 · Cited by 48 · see citing works
Self-citationBurian, A., Erdogan, Z., Jandrisits, C., & Zeitlinger, M. A. (2012) · Pharmacology · doi:10.1159/000342423 · Cited by 48 · see citing works
Nutritional Practices of Competitive British Wheelchair Games Players
Goosey-Tolfrey, V. L., & Crosland, J. (2010) · Adapted Physical Activity Quarterly · doi:10.1123/apaq.27.1.47 · Cited by 48 · see citing works
Takahama, U., Yamamoto, A., Hirota, S., & Oniki, T. (2003) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf021253+ · Cited by 46 · see citing works
The biological role of nitrate and nitrite: The times they are a-changin’
Lundberg, J. O., & Weitzberg, E. (2009) · Nitric Oxide · doi:10.1016/j.niox.2009.11.004 · Cited by 42 · see citing works
Self-citationIpe, D. S., & Ulett, G. C. (2016) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2016.06.013 · Cited by 42 · see citing works
Nitric Oxide in Plants: The Roles of Ascorbate and Hemoglobin
Wang, X., & Hargrove, M. S. (2013) · PLoS ONE · doi:10.1371/journal.pone.0082611 · Cited by 40 · see citing works
Torrecilla, C., Fernández-Concha, J., Cansino, J. R., Mainez, J. A., Amón, J. H., Costas, S., Angerri, O., Emiliani, E., Martín, M. A., Polo, M. Á. A., et al. (2020) · BMC Urology · doi:10.1186/s12894-020-00633-2 · Cited by 40 · see citing works
Wonoputri, V., Gunawan, C., Liu, S., Barraud, N., Yee, L. H., Lim, M., & Amal, R. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b07971 · Cited by 38 · see citing works
Management of recurrent urinary tract infections in healthy adult women.
Hickling, D. R., & Νitti, V. W. (2013) · Europe PMC (PubMed Central) · Cited by 38 · see citing works
Non-surgical management of recurrent urinary tract infections in women
Bergamin, P. A., & Kiosoglous, A. J. (2017) · Translational Andrology and Urology · doi:10.21037/tau.2017.06.09 · Cited by 38 · see citing works
Role of flavonoids and nitrates in cardiovascular health
Lovegrove, J. A., Stainer, A. R., & Hobbs, D. A. (2017) · Proceedings of The Nutrition Society · doi:10.1017/s0029665116002871 · Cited by 37 · see citing works
Shen, J., Walsh, B. J. C., Flores‐Mireles, A. L., Peng, H., Zhang, Y., Zhang, Y., Trinidad, J. C., Hultgren, S. J., & Giedroc, D. P. (2018) · ACS Chemical Biology · doi:10.1021/acschembio.8b00230 · Cited by 38 · see citing works
Lee, J. (2012) · Journal of Functional Foods · doi:10.1016/j.jff.2012.09.005 · Cited by 36 · see citing works
Fedrigo, N. H., Mazucheli, J., Albiero, J., Shinohara, D. R., Lodi, F. G., Machado, A. C. D. S., Sy, S. K. B., & Tognim, M. C. B. (2017) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02498-16 · Cited by 35 · see citing works
Rocha, B. S., Gago, B., Barbosa, R. M., Lundberg, J. O., Mann, G. E., Radí, R., & Laranjinha, J. A. N. (2012) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2012.12.017 · Cited by 34 · see citing works
Self-citationEffects of Vitamin C on High Blood Pressure Induced by Salt in Spontaneously Hypertensive Rats
NISHIKAWA, Y., Tatsumi, K., MATSUURA, T., Yamamoto, A., Nadamoto, T., & Urabe, K. (2003) · Journal of Nutritional Science and Vitaminology · doi:10.3177/jnsv.49.301 · Cited by 33 · see citing works
Akrawinthawong, K., Park, J. W., Piknova, B., Sibmooh, N., Fucharoen, S., & Schechter, A. N. (2014) · PLoS ONE · doi:10.1371/journal.pone.0092435 · Cited by 32 · see citing works
Basaqr, R., Skleres, M., Jayswal, R. D., & Thomas, D. T. (2020) · Clinical Nutrition · doi:10.1016/j.clnu.2020.10.012 · Cited by 32 · see citing works
Førde-Skjærvik, O., Skjærvik, O., Mørkøre, T., Thomassen, M. S., & Rørvik, K. (2005) · Aquaculture · doi:10.1016/j.aquaculture.2005.06.037 · Cited by 32 · see citing works
Uropathogenic Escherichia Coli and Tolerance to Nitric Oxide: The Role of Flavohemoglobin
Svensson, L., Marklund, B., Poljakovic, M., & Persson, K. (2006) · The Journal of Urology · doi:10.1016/s0022-5347(05)00144-8 · Cited by 32 · see citing works
Kishikawa, H., Ebberyd, A., Römling, U., Brauner, A., Lüthje, P., Lundberg, J. O., & Weitzberg, E. (2013) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2013.09.012 · Cited by 31 · see citing works
Self-citationA novel ureteral stent material with antibacterial and reducing encrustation properties
Zhao, J., Cao, Z., Ren, L., Chen, S., Zhang, B., Liu, R., & Yang, K. (2016) · Materials Science and Engineering C · doi:10.1016/j.msec.2016.04.103 · Cited by 32 · see citing works
Nitric Oxide Activates IL-6 Production and Expression in Human Renal Epithelial Cells
Demirel, I., Vumma, R., Mohlin, C., Svensson, L., Säve, S., & Persson, K. (2012) · American Journal of Nephrology · doi:10.1159/000345351 · Cited by 31 · see citing works
Inhibitory effect of nitrite on coagulation processes demonstrated by thrombelastography
Park, J., Piknova, B., Nghiem, K., Lozier, J. N., & Schechter, A. N. (2014) · Nitric Oxide · doi:10.1016/j.niox.2014.05.006 · Cited by 29 · see citing works
Zhou, Q., Xu, W., Wang, L., Wang, S., Pan, J. W., Fang, Y., Liang, H., Pan, C., & Xia, F. (2025) · ACS Nano · doi:10.1021/acsnano.4c14800 · Cited by 29 · see citing works
Herojeet, R., Dewangan, R., Naik, P. K., & Verma, J. R. (2023) · Scientific Reports · doi:10.1038/s41598-023-45622-1 · Cited by 28 · see citing works
Oleksy-Wawrzyniak, M., Junka, A., Brożyna, M., Migdał, P., Kwiek, B., Nowak, M., Mączyńska, B., & Bartoszewicz, M. (2021) · Pathogens · doi:10.3390/pathogens11010042 · Cited by 28 · see citing works
Yang, R., & Yuan, L. (2023) · Journal of Water Process Engineering · doi:10.1016/j.jwpe.2023.103871 · Cited by 28 · see citing works
Needs, S. H., Osborn, H. M. I., & Edwards, A. D. (2021) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2021.106199 · Cited by 25 · see citing works
A question of balance: achieving appropriate nutrient levels in biofortified staple crops
Sanahuja, G., Farré, G., Berman, J., Zorrilla‐López, U., Twyman, R. M., Capell, T., Christou, P., & Zhu, C. (2013) · Nutrition Research Reviews · doi:10.1017/s0954422413000176 · Cited by 25 · see citing works
Nitric Oxide, an Old Molecule With Noble Functions in Pseudomonas aeruginosa Biology
Toyofuku, M., & Yoon, S. S. (2018) · Advances in microbial physiology/Advances in Microbial Physiology · doi:10.1016/bs.ampbs.2018.01.005 · Cited by 23 · see citing works
Li, D., Nishi, S. K., Jovanovski, E., Zurbau, A., Komishon, A., Mejía, S. B., Khan, T. A., Sievenpiper, J. L., Miličić, D., Jenkins, A. L., et al. (2020) · Journal of Hypertension · doi:10.1097/hjh.0000000000002524 · Cited by 24 · see citing works
Takahama, U., Hirota, S., Yamamoto, A., & Oniki, T. (2003) · FEBS Letters · doi:10.1016/s0014-5793(03)00820-2 · Cited by 24 · see citing works
Recent insights into nitrite signaling processes in blood
Helms, C. C., Liu, X., & Kim‐Shapiro, D. B. (2016) · Biological Chemistry · doi:10.1515/hsz-2016-0263 · Cited by 23 · see citing works
Potential roles of nitrate and nitrite in nitric oxide metabolism in the eye
Park, J. W., Piknova, B., Jenkins, A. L., Hellinga, D., Parver, L. M., & Schechter, A. N. (2020) · Scientific Reports · doi:10.1038/s41598-020-69272-9 · Cited by 23 · see citing works
The Nitrate–Nitrite–Nitric Oxide Pathway in Mammals
Lundberg, J. O., Weitzberg, E., Shiva, S., & Gladwin, M. T. (2011) · Humana Press eBooks · doi:10.1007/978-1-60761-616-0_3 · Cited by 26 · see citing works
Self-citationHudson, R. E., Job, K. M., Sayre, C. L., Krepkova, L. V., Sherwin, C. M. T., & Enioutina, E. Y. (2022) · Frontiers in Pharmacology · doi:10.3389/fphar.2022.883216 · Cited by 22 · see citing works
Moroz, L. L., & Kohn, A. B. (2007) · Advances in experimental biology · doi:10.1016/s1872-2423(07)01001-0 · Cited by 20 · see citing works
Ascorbate mediates the non-enzymatic reduction of nitrite to nitric oxide
Du, J., Filipović, M. R., Wagner, B. A., & Buettner, G. R. (2023) · Advances in Redox Research · doi:10.1016/j.arres.2023.100079 · Cited by 20 · see citing works
Influence of Dietary Nitrate Supplementation on Exercise Tolerance and Performance
JONES, A. M., Vanhatalo, A., & Bailey, S. J. (2013) · Nestlé Nutrition Institute Workshop series · doi:10.1159/000345815 · Cited by 19 · see citing works
Yang, Y., Li, Y., Qu, Y., Wang, X., An, W., Li, Z., Han, Z., & Qin, L. (2020) · Journal of International Medical Research · doi:10.1177/0300060520902605 · Cited by 17 · see citing works
Nitric Oxide and Oxidative Stress Changes at Depth in Breath-Hold Diving
Cialoni, D., Brizzolari, A., Samaja, M., Bosco, G., Paganini, M., Pieri, M., Lancellotti, V., & Marroni, A. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2020.609642 · Cited by 18 · see citing works
Vatassery, G. T. (2004) · Neurochemical Research · doi:10.1007/s11064-004-6870-4 · Cited by 18 · see citing works
Vatassery, G. T., SantaCruz, K. S., DeMaster, E. G., Quach, H. T., & Smith, W. E. (2004) · Neurochemistry International · doi:10.1016/j.neuint.2004.06.009 · Cited by 18 · see citing works
Park, J. W., Piknova, B., Walter, P. J., Cai, H., Upanan, S., Thomas, S. M., Tunau-Spencer, K. J., & Schechter, A. N. (2023) · Scientific Reports · doi:10.1038/s41598-023-28190-2 · Cited by 18 · see citing works
Inhibition of Cancer Cell Replication by Inorganic Nitrite
Morcos, E., Carlsson, S., Weitzberg, E., Wiklund, N. P., & Lundberg, J. O. (2010) · Nutrition and Cancer · doi:10.1080/01635580903441170 · Cited by 17 · see citing works
Self-citationA graphene-based pH sensor on paper for human plasma and seawater
Vivaldi, F. M., Bonini, A., Melai, B., Poma, N. V., Kirchhain, A., Santalucia, D., Salvo, P., & Francesco, F. D. (2019) · Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) · doi:10.1109/embc.2019.8856991 · Cited by 16 · see citing works
Reactive Oxygen and Reactive Nitrogen Metabolites as Effector Molecules against Infectious Pathogens
Bogdan, C. (2014) · ASM Press eBooks · doi:10.1128/9781555817671.ch19 · Cited by 16 · see citing works
Takahama, U., Tanaka, M. H., & Hirota, S. (2008) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf8018535 · Cited by 16 · see citing works
Current Viewpoint on Female Urogenital Microbiome—The Cause or the Consequence?
Čeprnja, M., Hadžić, E., Oros, D., Melvan, E., Starčević, A., & Žučko, J. (2023) · Microorganisms · doi:10.3390/microorganisms11051207 · Cited by 15 · see citing works
Nanoscopic analysis on pH induced morphological changes of flagella in Escherichia coli
Chang, K., Cheng, S.-J., Chen, Y.-C., Huang, H.-R., & Liou, J.-W. (2012) · Journal of Microbiology Immunology and Infection · doi:10.1016/j.jmii.2012.08.004 · Cited by 15 · see citing works
Mechanisms of Nitrite Reduction in Ischemia in the Cardiovascular System
Webb, A., & Ahluwalia, A. (2010) · Elsevier eBooks · doi:10.1016/b978-0-12-373866-0.00017-4 · Cited by 15 · see citing works
Antimicrobial Mechanisms of Cranberry Juice
Rhee, K. Y., & Charles, M. (2004) · Clinical Infectious Diseases · doi:10.1086/423278 · Cited by 15 · see citing works
- Authors
- Carlsson, S., Wiklund, N. P., Engstrand, L., Weitzberg, E., & Lundberg, J. O. N.
- Affiliations
- Karolinska University Hospital (SE)
- Uppsala University Hospital (SE)
- Karolinska Institutet (SE)
- Corresponding author
- Carlsson, S.
- Venue
- Nitric Oxide
- Publisher
- Elsevier BV
- Published
- 2001-12
- Volume
- 5
- Issue
- 6
- Pages
- 580-586
- ISSN
- 1089-8603 (ISSN-L)
- OpenAlex
- W1974538071
- Cited by
- 189
- References
- 20
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 9 · 2023: 11 · 2022: 9 · 2021: 16 · 2020: 17 · 2019: 5 · 2018: 8 · 2017: 10 · 2016: 7 · 2015: 7 · 2014: 10 · 2013: 10 · 2012: 9
- FWCI
- 2.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nitric Oxide (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Vetenskapsrådet
- Topics
- Health Sciences › Medicine › Epidemiology › Urinary Tract Infections Management
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Urology › Urinary Bladder and Prostate Research
- Keywords
- nitrite, vitamin C, urine pH, urinary acidification, urinary tract infection, bacterial growth inhibition, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus saprophyticus, reactive nitrogen oxides, chemiluminescence
- MeSH terms
- Adult, Ascorbic Acid, Bacteriuria, Escherichia coli, Humans, Hydrogen-Ion Concentration, Middle Aged, Nitric Oxide, Nitrites, Pseudomonas aeruginosa, Staphylococcus
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
