Identification of skeletal muscle precursor cells in vivo by use of MyoD1 and myogenin probes
Abstract
The activation of mononuclear muscle precursor cells after crush injury to mouse tibialis anterior muscles was monitored in vivo by in situ hybridization with MyoD1 and myogenin probes. These genes are early markers of skeletal muscle differentiation and have been extensively studied in vitro. The role in vivo of these regulatory proteins during myogenesis of mature muscle has not been studied previously. MyoD1 and myogenin mRNA were present in occasional mononuclear cells of uninjured muscle. Increased MyoD1 and myogenin mRNA sequences in mononuclear cells were detected as early as 6 h after injury, peaked between 24 and 48 h, and thereafter declined to pre-injury levels at about 8 days. The mRNAs were detected in mononuclear cells throughout the muscle, with the majority of cells located some distance from the site of crush injury. The presence of MyoD1 and myogenin mRNA at 6 to 48 h indicates that transcription of these genes is occurring at the same time as replication of muscle precursor cells in vivo. At no time were significant levels of mRNA for these genes detected in myotubes. MyoD1 and myogenin provide precise markers for the very early identification and study of mononuclear skeletal muscle precursor cells in muscle regenerating in vivo.
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In MuSCs, the activation process is canonically characterized by expression of Myod1, loss of Spry1 and Pax7, and entry into the cell cycle.
“In MuSCs, the activation process is canonically characterized by expression of Myod1 (Grounds et al., 1992; Yablonka-Reuveni and Rivera, 1994), loss of Spry1 and Pax7, and entry into the cell cycle (Shea et al., 2010).”
From Aging induces aberrant state transition kinetics in murine muscle stem cells (2020) · cites this paper as Grounds et al., 1992
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- Authors
- Grounds, M. D., Garrett, K. L., Lai, M. C., Wright, W. E., & Beilharz, M. W.
- Affiliations
- The University of Western Australia (AU)
- The University of Texas Southwestern Medical Center (US)
- Venue
- Cell & Tissue Research
- Published
- 1992-01
- Volume
- 267
- Issue
- 1
- Pages
- 99-104
- ISSN
- 0302-766X (ISSN-L)
- 1432-0878
- OpenAlex
- W2060223045
- Cited by
- 313
- References
- 28
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 1 · 2023: 4 · 2022: 6 · 2021: 10 · 2020: 6 · 2019: 6 · 2018: 5 · 2017: 6 · 2016: 9 · 2015: 13 · 2014: 5 · 2013: 12 · 2012: 10
- FWCI
- 3.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cell and Tissue Research (publishedVersion)
- PubMed (publishedVersion)
- UWA Profiles and Research Repository (University of Western Australia) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- MyoD, myogenin, muscle regeneration, muscle crush injury, tibialis anterior, myogenesis, in situ hybridization, myoblasts, myotubes, gene expression biomarkers
- MeSH terms
- Animals, Male, Muscle Proteins, Muscles, Nuclear Proteins, Phosphoproteins, Regeneration, RNA, Messenger, Stem Cells, Molecular Probes, Biomarkers, MyoD Protein, Myogenin, Mice
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