Cited by 313Muscle Physiology and DisordersCongenital heart defects researchDevelopmental Biology and Gene Regulation

Identification of skeletal muscle precursor cells in vivo by use of MyoD1 and myogenin probes

Grounds, Miranda D., Garrett, Kerryn L., Lai, May C., Wright, Woodring E., Beilharz, Manfred W.·1992·Cell & Tissue Research

Abstract

The activation of mononuclear muscle precursor cells after crush injury to mouse tibialis anterior muscles was monitored in vivo by in situ hybridization with MyoD1 and myogenin probes. These genes are early markers of skeletal muscle differentiation and have been extensively studied in vitro. The role in vivo of these regulatory proteins during myogenesis of mature muscle has not been studied previously. MyoD1 and myogenin mRNA were present in occasional mononuclear cells of uninjured muscle. Increased MyoD1 and myogenin mRNA sequences in mononuclear cells were detected as early as 6 h after injury, peaked between 24 and 48 h, and thereafter declined to pre-injury levels at about 8 days. The mRNAs were detected in mononuclear cells throughout the muscle, with the majority of cells located some distance from the site of crush injury. The presence of MyoD1 and myogenin mRNA at 6 to 48 h indicates that transcription of these genes is occurring at the same time as replication of muscle precursor cells in vivo. At no time were significant levels of mRNA for these genes detected in myotubes. MyoD1 and myogenin provide precise markers for the very early identification and study of mononuclear skeletal muscle precursor cells in muscle regenerating in vivo.

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Crossref · 10.1007/bf00318695

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  • In MuSCs, the activation process is canonically characterized by expression of Myod1, loss of Spry1 and Pax7, and entry into the cell cycle.

    “In MuSCs, the activation process is canonically characterized by expression of Myod1 (Grounds et al., 1992; Yablonka-Reuveni and Rivera, 1994), loss of Spry1 and Pax7, and entry into the cell cycle (Shea et al., 2010).”

    From Aging induces aberrant state transition kinetics in murine muscle stem cells (2020) · cites this paper as Grounds et al., 1992

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Authors
Grounds, M. D., Garrett, K. L., Lai, M. C., Wright, W. E., & Beilharz, M. W.
Affiliations
  • The University of Western Australia (AU)
  • The University of Texas Southwestern Medical Center (US)
Venue
Cell & Tissue Research
Published
1992-01
Volume
267
Issue
1
Pages
99-104
ISSN
  • 0302-766X (ISSN-L)
  • 1432-0878
OpenAlex
W2060223045
Cited by
313
References
28
Citations by year
2026: 3 · 2025: 1 · 2024: 1 · 2023: 4 · 2022: 6 · 2021: 10 · 2020: 6 · 2019: 6 · 2018: 5 · 2017: 6 · 2016: 9 · 2015: 13 · 2014: 5 · 2013: 12 · 2012: 10
FWCI
3.17
Type
journal article
Language
en
Open access
closed
Locations
  • Cell and Tissue Research (publishedVersion)
  • PubMed (publishedVersion)
  • UWA Profiles and Research Repository (University of Western Australia) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
MyoD, myogenin, muscle regeneration, muscle crush injury, tibialis anterior, myogenesis, in situ hybridization, myoblasts, myotubes, gene expression biomarkers
MeSH terms
Animals, Male, Muscle Proteins, Muscles, Nuclear Proteins, Phosphoproteins, Regeneration, RNA, Messenger, Stem Cells, Molecular Probes, Biomarkers, MyoD Protein, Myogenin, Mice

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