Differences in subcellular distribution and toxicity of green and red emitting CdTe quantum dots
Abstract
Quantum dots (QDs) are emerging as alternative or complementary tools to the organic fluorescent dyes currently used in bioimaging. QDs hold several advantages over conventional fluorescent dyes including greater photostability and a wider range of excitation/emission wavelengths. However, recent work suggests that QDs exert deleterious effects on cellular processes. This study examined the subcellular localization and toxicity of cadmium telluride (CdTe) QDs and pharmacological means of preventing QD-induced cell death. The localization of CdTe QDs was found to depend upon QD size. CdTe QDs exhibited marked cytotoxicity in PC12 and N9 cells at concentrations as low as 10 microg/ml in chronic treatment paradigms. QD-induced cell death was characterized by chromatin condensation and membrane blebbing and was more pronounced with small (2r=2.2+/-0.1 nm), green emitting positively charged QDs than large (2r=5.2+/-0.1 nm), equally charged red emitting QDs. Pretreatment of cells with the antioxidant N-acetylcysteine and with bovine serum albumin, but not Trolox, significantly reduced the QD-induced cell death. These findings suggest that the size of QDs contributes to their subcellular distribution and that drugs can alter QD-induced cytotoxicity.
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- Authors
- Lovrić, J., Bazzi, H. S., Cuie, Y., Fortin, G. R. A., Winnik, F. M., & Maysinger, D.
- Affiliations
- McGill University (CA)
- Université de Montréal (CA)
- Venue
- Journal of Molecular Medicine
- Published
- 2005-02-02
- Volume
- 83
- Issue
- 5
- Pages
- 377-385
- ISSN
- 0946-2716 (ISSN-L)
- 1432-1440
- OpenAlex
- W2106955414
- Cited by
- 815
- References
- 34
- Citations by year
- 2026: 8 · 2025: 10 · 2024: 19 · 2023: 29 · 2022: 22 · 2021: 25 · 2020: 31 · 2019: 37 · 2018: 35 · 2017: 37 · 2016: 42 · 2015: 45 · 2014: 51 · 2013: 53 · 2012: 65
- FWCI
- 24.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Molecular Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Quantum Dots Synthesis And Properties
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Keywords
- cadmium telluride quantum dots, subcellular localization, quantum dot size, N-acetylcysteine, bovine serum albumin, Trolox, antioxidant pretreatment, cell death, chromatin condensation, membrane blebbing, PC12 cells, N9 cells, fluorescent dyes
- MeSH terms
- Acetylcysteine, Animals, Antioxidants, Cattle, Cell Line, Fluorescent Dyes, Semiconductors, Serum Albumin, Bovine, Spectrometry, Fluorescence, Tellurium, PC12 Cells, Cell Death, Microglia, Microscopy, Confocal, Cadmium Compounds, Quantum Dots, Cell Line, Tumor, Nanostructures, Mice, Rats
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