The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Transcriptomic profiling of pancreatic alpha, beta and delta cell populations identifies delta cells as a principal target for ghrelin in mouse islets
Open accessAbstract
AIMS/HYPOTHESIS: Intra-islet and gut-islet crosstalk are critical in orchestrating basal and postprandial metabolism. The aim of this study was to identify regulatory proteins and receptors underlying somatostatin secretion though the use of transcriptomic comparison of purified murine alpha, beta and delta cells. METHODS: Sst-Cre mice crossed with fluorescent reporters were used to identify delta cells, while Glu-Venus (with Venus reported under the control of the Glu [also known as Gcg] promoter) mice were used to identify alpha and beta cells. Alpha, beta and delta cells were purified using flow cytometry and analysed by RNA sequencing. The role of the ghrelin receptor was validated by imaging delta cell calcium concentrations using islets with delta cell restricted expression of the calcium reporter GCaMP3, and in perfused mouse pancreases. RESULTS: A database was constructed of all genes expressed in alpha, beta and delta cells. The gene encoding the ghrelin receptor, Ghsr, was highlighted as being highly expressed and enriched in delta cells. Activation of the ghrelin receptor raised cytosolic calcium levels in primary pancreatic delta cells and enhanced somatostatin secretion in perfused pancreases, correlating with a decrease in insulin and glucagon release. The inhibition of insulin secretion by ghrelin was prevented by somatostatin receptor antagonism. CONCLUSIONS/INTERPRETATION: Our transcriptomic database of genes expressed in the principal islet cell populations will facilitate rational drug design to target specific islet cell types. The present study indicates that ghrelin acts specifically on delta cells within pancreatic islets to elicit somatostatin secretion, which in turn inhibits insulin and glucagon release. This highlights a potential role for ghrelin in the control of glucose metabolism.
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“Both mouse (3, 146) and human (68, 475) β-cells express Hcn/HCN2-4.”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [3]
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“which is expressed in mouse β-cells(3, 146).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [3]
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“However, these are only expressed at low levels in mouse and human β-cells alike (3, 68, 146, 475).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [3]
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“Both human and mouse β-cells also express Slc6a17/SLC6a17 (3, 68, 146, 475) that encodes a synaptic vesicle glycine transporter (502).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [3]
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“In human β-cells, only SYN1 is expressed, whereas mouse β-cells express both Syn1 and Syn2 (3, 68, 146, 475).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [3]
Experimental — accuracy not yet measured
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“These are both expressed at low levels in mouse and human β-cells (3, 68, 146, 475).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [3]
Experimental — accuracy not yet measuredThe check wasn’t confident either way, so it gives no verdict — never disputed.
In the citing paper
“Mouse and human β-cells express CAPS1 (Cadps/CADPS) and low amounts of CAPS2 (Cadps2/CADPS2) (3, 68, 146, 475).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [3]
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Synthèse de peptides bioactifs inspirés des venins
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Metadata
- Authors
- Adriaenssens, A. E., Svendsen, B., Lam, B. Y. H., Yeo, G. S. H., Holst, J. J., Reimann, F., & Gribble, F. M.
- Affiliations
- Wellcome/MRC Institute of Metabolic Science (GB)
- University of Copenhagen (DK)
- Corresponding author
- Reimann, Frank
- Gribble, Fiona M.
- ORCID
- Venue
- Diabetologia
- Published
- 2016-07-07
- Volume
- 59
- Issue
- 10
- Pages
- 2156-2165
- ISSN
- 0012-186X (ISSN-L)
- 1432-0428
- PMC
- PMC5016554
- OpenAlex
- W2460804009
- Cited by
- 223
- References
- 48
- Citations by year
- 2026: 14 · 2025: 21 · 2024: 21 · 2023: 24 · 2022: 21 · 2021: 29 · 2020: 23 · 2019: 28 · 2018: 24 · 2017: 16 · 2016: 2
- FWCI
- 25.85
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Diabetologia (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, cc-by)
- Apollo (University of Cambridge) (submittedVersion, cc-by)
- Apollo (cc-by)
- Funders
- Wellcome Trust (106262/Z/14/Z)
- Medical Research Council (MRC_MC_UU_12012/5)
- Novo Nordisk Foundation
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- ghrelin, growth hormone secretagogue receptor, pancreatic delta cells, somatostatin secretion, pancreatic islets, alpha cells, pancreatic beta cells, insulin secretion, glucagon secretion, glucose metabolism
- MeSH terms
- Animals, Calcium, Glucagon, Insulin, Islets of Langerhans, Male, Mice, Inbred C57BL, Mice, Transgenic, Somatostatin-Secreting Cells, Reverse Transcriptase Polymerase Chain Reaction, Glucagon-Secreting Cells, Insulin-Secreting Cells, Mice, Ghrelin, Transcriptome
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