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Probiotic lactobacilli inhibit early stages of Candida albicans biofilm development by reducing their growth, cell adhesion, and filamentation
Open accessAbstract
We evaluated the inhibitory effects of the probiotic Lactobacillus species on different phases of Candida albicans biofilm development. Quantification of biofilm growth and ultrastructural analyses were performed on C. albicans biofilms treated with Lactobacillus rhamnosus, Lactobacillus casei, and Lactobacillus acidophilus planktonic cell suspensions as well as their supernatants. Planktonic lactobacilli induced a significant reduction (p < 0.05) in the number of biofilm cells (25.5-61.8 %) depending on the probiotic strain and the biofilm phase. L. rhamnosus supernatants had no significant effect on the mature biofilm (p > 0.05), but significantly reduced the early stages of Candida biofilm formation (p < 0.01). Microscopic analyses revealed that L. rhamnosus suspensions reduced Candida hyphal differentiation, leading to a predominance of budding growth. All lactobacilli negatively impacted C. albicans yeast-to-hyphae differentiation and biofilm formation. The inhibitory effects of the probiotic Lactobacillus on C. albicans entailed both cell-cell interactions and secretion of exometabolites that may impact on pathogenic attributes associated with C. albicans colonization on host surfaces and yeast filamentation. This study clarifies, for the first time, the mechanics of how Lactobacillus species may antagonize C. albicans host colonization. Our data elucidate the inhibitory mechanisms that define the probiotic candicidal activity of lactobacilli, thus supporting their utility as an adjunctive therapeutic mode against mucosal candidal infections.
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“In vitro studies have shown that exogenous Lactobacilli inhibit the formation of C. albicans biofilm [39].”
From Treatment of Vulvovaginal Candidiasis—An Overview of Guidelines and the Latest Treatment Methods (2023) · cites this paper as [39]
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Metadata
- Authors
- Matsubara, V. H., Wang, Y., Bandara, H. M. H. N., Mayer, M. P. A., & Samaranayake, L. P.
- Affiliations
- The University of Queensland (AU)
- Corresponding author
- Wang, Yi
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2016-04-18
- Volume
- 100
- Issue
- 14
- Pages
- 6415-6426
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W2341432746
- Cited by
- 206
- References
- 57
- Citations by year
- 2026: 15 · 2025: 19 · 2024: 17 · 2023: 23 · 2022: 26 · 2021: 28 · 2020: 22 · 2019: 20 · 2018: 15 · 2017: 15 · 2016: 6
- FWCI
- 23.51
- Type
- journal article
- Language
- en
- Open access
- Licence
- other-oa
- www.springer.com/tdm (tdm)
- Locations
- Applied Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Bristol Research (University of Bristol) (publishedVersion, other-oa)
- Explore Bristol Research (submittedVersion, other-oa)
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- probiotics, Lactobacillus, Candida albicans, biofilm formation, filamentation, cell adhesion, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus acidophilus, exometabolites, candidiasis, host colonization, antagonism
- MeSH terms
- Antibiosis, Candida albicans, Cell Adhesion, Culture Media, Lactobacillus acidophilus, Biofilms, Probiotics, Hyphae, Lacticaseibacillus rhamnosus
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