The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato
Open accessPublished version of a preprint
Abstract
Key message The StGBSSI gene was successfully and precisely edited in the tetraploid potato using gene and base-editing strategies, leading to plants with impaired amylose biosynthesis. Genome editing has recently become a method of choice for basic research and functional genomics, and holds great potential for molecular plant-breeding applications. The powerful CRISPR-Cas9 system that typically produces double-strand DNA breaks is mainly used to generate knockout mutants. Recently, the development of base editors has broadened the scope of genome editing, allowing precise and efficient nucleotide substitutions. In this study, we produced mutants in two cultivated elite cultivars of the tetraploid potato (Solanum tuberosum) using stable or transient expression of the CRISPR-Cas9 components to knock out the amylose-producing StGBSSI gene. We set up a rapid, highly sensitive and cost-effective screening strategy based on high-resolution melting analysis followed by direct Sanger sequencing and trace chromatogram analysis. Most mutations consisted of small indels, but unwanted insertions of plasmid DNA were also observed. We successfully created tetra-allelic mutants with impaired amylose biosynthesis, confirming the loss of function of the StGBSSI protein. The second main objective of this work was to demonstrate the proof of concept of CRISPR-Cas9 base editing in the tetraploid potato by targeting two loci encoding catalytic motifs of the StGBSSI enzyme. Using a cytidine base editor (CBE), we efficiently and precisely induced DNA substitutions in the KTGGL-encoding locus, leading to discrete variation in the amino acid sequence and generating a loss-of-function allele. The successful application of base editing in the tetraploid potato opens up new avenues for genome engineering in this species.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Gene and base-editing strategies have precisely edited the granule bound starch synthase gene (StGBSSI) in tetraploid potato and impaired amylose biosynthesis.
“The gene and base-editing strategies has precisely edited granule bound starch synthase gene (StGBSSI) gene in the tetraploid potato and with impaired amylose biosynthesis (Veillet et al., 2019).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Veillet et al., 2019
Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116 · see citing works
Primer3—new capabilities and interfaces
Untergasser, A., Cutcutache, I., Kõressaar, T., Ye, J., Faircloth, B., Remm, M., & Rozen, S. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks596 · Cited by 9,165 · see citing works
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis
Yoo, S.-D., Cho, Y.-H., & Sheen, J. (2007) · Nature Protocols · doi:10.1038/nprot.2007.199 · Cited by 5,104 · see citing works
Comparative Protein Structure Modeling Using MODELLER
Webb, B., & Šali, A. (2016) · Current Protocols in Bioinformatics · doi:10.1002/cpbi.3 · Cited by 4,349 · see citing works
Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017) · Nature · doi:10.1038/nature24644 · Cited by 4,184 · see citing works
Statistical potential for assessment and prediction of protein structures
Shen, M., & Šali, A. (2006) · Protein Science · doi:10.1110/ps.062416606 · Cited by 2,294 · see citing works
Haeussler, M., Schönig, K., Eckert, H., Eschstruth, A., Mianné, J., Renaud, J.-B., Schneider-Maunoury, S., Shkumatava, A., Teboul, L., Kent, J., et al. (2016) · Genome biology · doi:10.1186/s13059-016-1012-2 · Cited by 1,756 · see citing works
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
Hu, J. H., Miller, S. M., Geurts, M. H., Tang, W., Chen, L., Sun, N., Zeina, C. M., Gao, X., Rees, H. A., Lin, Z., et al. (2018) · Nature · doi:10.1038/nature26155 · Cited by 1,552 · see citing works
Krieger, E., Joo, K., Lee, J., Lee, J., Raman, S., Thompson, J., Tyka, M., Baker, D. A., & Karplus, K. J. (2009) · Proteins Structure Function and Bioinformatics · doi:10.1002/prot.22570 · Cited by 1,396 · see citing works
Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems
Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki, M., Hara, K. Y., et al. (2016) · Science · doi:10.1126/science.aaf8729 · Cited by 1,349 · see citing works
Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
Anders, C., Niewoehner, O., Duerst, A., & Jínek, M. (2014) · Nature · doi:10.1038/nature13579 · Cited by 1,319 · see citing works
Source-to-sink transport of sugar and regulation by environmental factors
Lemoine, R., Camera, S. L., Atanassova, R., Dédaldéchamp, F., Allario, T., Pourtau, N., Bonnemain, J., Laloi, M., Coutos‐Thévenot, P., Maurousset, L., et al. (2013) · Frontiers in Plant Science · doi:10.3389/fpls.2013.00272 · Cited by 1,241 · see citing works
The CRISPR/Cas9 system for plant genome editing and beyond
Bortesi, L., & Fischer, R. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.12.006 · Cited by 1,234 · see citing works
DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins
Woo, J. W., Kim, J., Kwon, S. I., Corvalán, C., Cho, S. W., Kim, H., Kim, S.-G., Kim, S.-T., Choe, S., & Kim, J.-S. (2015) · Nature Biotechnology · doi:10.1038/nbt.3389 · Cited by 1,219 · see citing works
Engineered CRISPR-Cas9 nuclease with expanded targeting space
Nishimasu, H., Shi, X., Ishiguro, S., Gao, L., Hirano, S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H., et al. (2018) · Science · doi:10.1126/science.aas9129 · Cited by 1,100 · see citing works
Starch: its metabolism, evolution, and biotechnological modification in plants
Zeeman, S. C., Kossmann, J., & Smith, A. M. (2010) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042809-112301 · Cited by 1,095 · see citing works
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
Liang, Z., Chen, K., Li, T., Zhang, Y., Wang, Y., Zhao, Q., Liu, J., Zhang, H., Liu, C., Ran, Y., et al. (2017) · Nature Communications · doi:10.1038/ncomms14261 · Cited by 964 · see citing works
Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion
Zong, Y., Wang, Y., Li, C., Zhang, R., Chen, K., Ran, Y., Qiu, J., Wang, D., & Gao, C. (2017) · Nature Biotechnology · doi:10.1038/nbt.3811 · Cited by 875 · see citing works
Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.3833 · Cited by 812 · see citing works
Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion
Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D., & Kamoun, S. (2017) · Scientific Reports · doi:10.1038/s41598-017-00578-x · Cited by 769 · see citing works
Peng, A., Chen, S., Lei, T., Xu, L., He, Y., Liu, W., Yao, L., & Zou, X. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12733 · Cited by 732 · see citing works
Fauser, F., Schiml, S., & Puchta, H. (2014) · The Plant Journal · doi:10.1111/tpj.12554 · Cited by 704 · see citing works
Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice
Jin, S., Zong, Y., Gao, Q., Zhu, Z., Wang, Y., Qin, P., Liang, C., Wang, D., Qiu, J.-L., Zhang, F., et al. (2019) · Science · doi:10.1126/science.aaw7166 · Cited by 665 · see citing works
Demirer, G. S., Zhang, H., Matos, J. L., Goh, N. S., Cunningham, F. J., Sung, Y., Chang, R., Aditham, A. J., Chio, L., Cho, M., et al. (2019) · Nature Nanotechnology · doi:10.1038/s41565-019-0382-5 · Cited by 640 · see citing works
The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution
Puchta, H. (2004) · Journal of Experimental Botany · doi:10.1093/jxb/eri025 · Cited by 603 · see citing works
Andersson, M., Turesson, H., Nicolia, A., Fält, A., Samuelsson, M., & Hofvander, P. (2016) · Plant Cell Reports · doi:10.1007/s00299-016-2062-3 · Cited by 540 · see citing works
CRISPR/Cpf1-mediated DNA-free plant genome editing
Kim, H., Kim, S., Ryu, J., Kang, B., Kim, J., & Kim, S. (2017) · Nature Communications · doi:10.1038/ncomms14406 · Cited by 513 · see citing works
Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
Li, C., Zong, Y., Wang, Y., Jin, S., Zhang, D., Song, Q., Zhang, R., & Gao, C. (2018) · Genome biology · doi:10.1186/s13059-018-1443-z · Cited by 499 · see citing works
CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications
Ma, X., Zhu, Q., Chen, Y., & Liu, Y. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.04.009 · Cited by 480 · see citing works
Somaclonal variations and their applications in horticultural crops improvement
Krishna, H., Alizadeh, M., Singh, D., Singh, U., Chauhan, N., Eftekhari, M., & Sadh, R. K. (2016) · 3 Biotech · doi:10.1007/s13205-016-0389-7 · Cited by 436 · see citing works
Genome editing in potato via CRISPR‐Cas9 ribonucleoprotein delivery
Andersson, M., Turesson, H., Olsson, N., Fält, A., Ohlsson, P., González, M. N., Samuelsson, M., & Hofvander, P. (2018) · Physiologia Plantarum · doi:10.1111/ppl.12731 · Cited by 435 · see citing works
Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A
Zong, Y., Song, Q., Li, C., Jin, S., Zhang, D., Wang, Y., Qiu, J., & Gao, C. (2018) · Nature Biotechnology · doi:10.1038/nbt.4261 · Cited by 433 · see citing works
Improving cold storage and processing traits in potato through targeted gene knockout
Clasen, B. M., Stoddard, T., Luo, S. J., Demorest, Z. L., Li, J., Cédrone, F., Tibebu, R., Davison, S., Ray, E. E., Daulhac, A., et al. (2015) · Plant Biotechnology Journal · doi:10.1111/pbi.12370 · Cited by 414 · see citing works
Pan, C., Ye, L., Li, Q., Liu, X., He, Y., Wang, J., Chen, L., & Lu, G. (2016) · Scientific Reports · doi:10.1038/srep24765 · Cited by 381 · see citing works
Veillet, F., Perrot, L., Chauvin, L., Kermarrec, M.-P., Guyon‐Debast, A., Chauvin, J.-É., Nogué, F., & Mazier, M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20020402 · Cited by 370 · see citing works
Self-citationCapture of genomic and T‐DNA sequences during double‐strand break repair in somatic plant cells
Salomon, S., & Puchta, H. (1998) · The EMBO Journal · doi:10.1093/emboj/17.20.6086 · Cited by 361 · see citing works
Statistical potentials for fold assessment
Melo, F., Sánchez, R., & Šali, A. (2002) · Protein Science · doi:10.1002/pro.110430 · Cited by 345 · see citing works
Butler, N., Baltes, N. J., Voytas, D. F., & Douches, D. S. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01045 · Cited by 329 · see citing works
Comparative protein structure modeling by iterative alignment, model building and model assessment
John, B. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg460 · Cited by 312 · see citing works
Engineering herbicide-resistant watermelon variety through CRISPR/Cas9-mediated base-editing
Tian, S., Jiang, L., Cui, X., Zhang, J., Guo, S., Li, M., Zhang, H., Ren, Y., Gong, G., Zong, M., et al. (2018) · Plant Cell Reports · doi:10.1007/s00299-018-2299-0 · Cited by 307 · see citing works
Precision genome engineering through adenine base editing in plants
Kang, B., Yun, J., Kim, S., Shin, Y., Ryu, J., Choi, M., Woo, J. W., & Kim, J. (2018) · Nature Plants · doi:10.1038/s41477-018-0178-x · Cited by 306 · see citing works
Tang, X. D., Liu, G., Zhou, J., Ren, Q., You, Q., Tian, L., Xin, X., Zhong, Z., Liu, B., Zheng, X., et al. (2018) · Genome biology · doi:10.1186/s13059-018-1458-5 · Cited by 305 · see citing works
Inference of CRISPR Edits from Sanger Trace DataPreprint
Hsiau, T., Conant, D., Rossi, N., Maures, T. J., Waite, K., Yang, J. L., Joshi, S., Kelso, R., Holden, K., Enzmann, B. L., et al. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/251082 · Cited by 302 · see citing works
Leveraging Rules of Nonsense-Mediated mRNA Decay for Genome Engineering and Personalized Medicine
Popp, M. W., & Maquat, L. E. (2016) · Cell · doi:10.1016/j.cell.2016.05.053 · Cited by 293 · see citing works
Visser, R. G. F., Somhorst, I., Kuipers, G. J., Ruys, N. J., Feenstra, W. J., & Jacobsen, E. (1991) · Molecular and General Genetics MGG · doi:10.1007/bf00269861 · Cited by 289 · see citing works
Roldán, I., Wattebled, F., Lucas, M. M., Delvallé, D., Planchot, V., Jiménez, S., Pérez, R., Ball, S. G., d’Hulst, C., & Mérida, Á. (2007) · The Plant Journal · doi:10.1111/j.1365-313x.2006.02968.x · Cited by 283 · see citing works
Efficient targeted mutagenesis in potato by the CRISPR/Cas9 system
Wang, S., Zhang, S., Wang, W., Xiong, X., Meng, F., & Cui, X. (2015) · Plant Cell Reports · doi:10.1007/s00299-015-1816-7 · Cited by 275 · see citing works
Steinert, J., Schiml, S., Fauser, F., & Puchta, H. (2015) · The Plant Journal · doi:10.1111/tpj.13078 · Cited by 256 · see citing works
Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture
Ricroch, A., Clairand, P., & Harwood, W. (2017) · Emerging Topics in Life Sciences · doi:10.1042/etls20170085 · Cited by 252 · see citing works
Adaptation in CRISPR-Cas Systems
Sternberg, S. H., Richter, H., Charpentier, E., & Qimron, U. (2016) · Molecular Cell · doi:10.1016/j.molcel.2016.01.030 · Cited by 251 · see citing works
Butler, N., Atkins, P., Voytas, D. F., & Douches, D. S. (2015) · PLoS ONE · doi:10.1371/journal.pone.0144591 · Cited by 250 · see citing works
Precise A·T to G·C Base Editing in the Rice Genome
Hua, K., Tao, X., Yuan, F., Wang, D., & Zhu, J. (2018) · Molecular Plant · doi:10.1016/j.molp.2018.02.007 · Cited by 232 · see citing works
Progress in understanding the biosynthesis of amylose
Ball, S. G., de Wal, M. H. B. J. V., & Visser, R. G. F. (1998) · Trends in Plant Science · doi:10.1016/s1360-1385(98)01342-9 · Cited by 224 · see citing works
Isolation of an amylose-free starch mutant of the potato (Solanum tuberosum L.)
Hovenkamp-Hermelink, J., Jacobsen, E., Ponstein, A. S., Visser, R. G. F., Vos‐Scheperkeuter, G. H., Bijmolt, E. W., de Vries, J. N., Witholt, B., & Feenstra, W. J. (1987) · Theoretical and Applied Genetics · doi:10.1007/bf00249167 · Cited by 216 · see citing works
Expanding the base editing scope in rice by using Cas9 variants
Hua, K., Tao, X., & Zhu, J. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12993 · Cited by 214 · see citing works
Highly Efficient A·T to G·C Base Editing by Cas9n-Guided tRNA Adenosine Deaminase in Rice
Yan, F., Kuang, Y., Ren, B., Wang, J., Zhang, D., Lin, H., Yang, B., Zhou, X., & Zhou, H. (2018) · Molecular Plant · doi:10.1016/j.molp.2018.02.008 · Cited by 205 · see citing works
Delvallé, D., Dumez, S., Wattebled, F., Roldán, I., Planchot, V., Berbezy, P., Colonna, P., Vyas, D., Chatterjee, M., Ball, S. G., et al. (2005) · The Plant Journal · doi:10.1111/j.1365-313x.2005.02462.x · Cited by 195 · see citing works
Genome editing in plants by engineered CRISPR–Cas9 recognizing NG PAM
Endo, M., Mikami, M., Endo, A., Kaya, H., Itoh, T., Nishimasu, H., Nureki, O., & Toki, S. (2018) · Nature Plants · doi:10.1038/s41477-018-0321-8 · Cited by 185 · see citing works
Chen, L., Li, W., Katin‐Grazzini, L., Ding, J., Gu, X., Li, Y., Gu, T., Wang, R., Lin, X., Deng, Z., et al. (2018) · Horticulture Research · doi:10.1038/s41438-018-0023-4 · Cited by 183 · see citing works
Bastet, A., Zafirov, D., Giovinazzo, N., Guyon‐Debast, A., Nogué, F., Robaglia, C., & Gallois, J. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13096 · Cited by 182 · see citing works
Self-citationGelatinization and Pasting Properties of Waxy and Non‐waxy Rice Starches
Park, I., Ibáñez, A. M., Zhong, F., & Shoemaker, C. F. (2007) · Starch - Stärke · doi:10.1002/star.200600570 · Cited by 181 · see citing works
Applications of New Breeding Technologies for Potato Improvement
Hameed, A., Zaidi, S. S., Shakir, S., & Mansoor, S. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00925 · Cited by 173 · see citing works
Kuipers, A., Jacobsen, E., & Visser, R. G. F. (1994) · The Plant Cell · doi:10.1105/tpc.6.1.43 · Cited by 166 · see citing works
Accelerated ex situ breeding of GBSS - and PTST1 -edited cassava for modified starch
Bull, S. E., Seung, D., Chanez, C., Mehta, D., Kuon, J., Truernit, E., Hochmuth, A., Zurkirchen, I., Zeeman, S. C., Gruissem, W., et al. (2018) · Science Advances · doi:10.1126/sciadv.aat6086 · Cited by 166 · see citing works
Mechanism of Starch Biosynthesis
de Fekete, M. A. R., Leloir, L. F., & Cardini, C. E. (1960) · Nature · doi:10.1038/187918a0 · Cited by 141 · see citing works
Regeneration of Solanum tuberosum Plants from Protoplasts Induces Widespread Genome Instability
Fossi, M., Amundson, K., Kuppu, S., Britt, A., & Comai, L. (2019) · PLANT PHYSIOLOGY · doi:10.1104/pp.18.00906 · Cited by 136 · see citing works
Transforming plant biology and breeding with CRISPR /Cas9, Cas12 and Cas13
Schindele, P., Wolter, F., & Puchta, H. (2018) · FEBS Letters · doi:10.1002/1873-3468.13073 · Cited by 104 · see citing works
Precision breeding for novel starch variants in potato
Muth, J. R., Hartje, S., Twyman, R. M., Hofferbert, H.-R., Tacke, E., & Prüfer, D. (2008) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2008.00340.x · Cited by 87 · see citing works
Starches—from current models to genetic engineering
Sonnewald, U., & Koßmann, J. (2012) · Plant Biotechnology Journal · doi:10.1111/pbi.12029 · Cited by 87 · see citing works
xC as9 expands the scope of genome editing with reduced efficiency in rice
Wang, J., Meng, X., Hu, X., Sun, T., Li, J., Wang, K., & Yu, H. J. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.13053 · Cited by 87 · see citing works
Potato starch synthases: Functions and relationships
Firouzabadi, F. N., & Visser, R. G. F. (2017) · Biochemistry and Biophysics Reports · doi:10.1016/j.bbrep.2017.02.004 · Cited by 76 · see citing works
Veillet, F., Gaillard, C., Coutos‐Thévenot, P., & Camera, S. L. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01899 · Cited by 73 · see citing works
Self-citationA novel selection system for potato transformation using a mutated AHAS gene
Andersson, M., Trifonova, A., Andersson, A.-B., Johansson, M., B�low, L., & Hofvander, P. (2003) · Plant Cell Reports · doi:10.1007/s00299-003-0684-8 · Cited by 65 · see citing works
Helle, S., Bray, F., Verbeke, J., Flament, S., Courseaux, A., Facon, M., Tokarski, C., Rolando, C. H., & Szydlowski, N. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00746 · Cited by 53 · see citing works
Self-citationCutting Edge Genetics: CRISPR/Cas9 Editing of Plant Genomes
Soyars, C. L., Peterson, B. A., Burr, C. A., & Nimchuk, Z. L. (2018) · Plant and Cell Physiology · doi:10.1093/pcp/pcy079 · Cited by 50 · see citing works
Cheng, J. S., Khan, M. A., Qiu, W., Li, J., Zhou, H., Zhang, Q., Guo, W., Zhu, T., Peng, J. H., Sun, F., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0030088 · Cited by 49 · see citing works
A Dual sgRNA Approach for Functional Genomics in Arabidopsis thaliana
Pauwels, L., Clercq, R. D., Goossens, J., Iñigo, S., Williams, C. J., Ron, M., Britt, A., & Goossens, A. (2018) · G3 Genes Genomes Genetics · doi:10.1534/g3.118.200046 · Cited by 38 · see citing works
Masson, J., Lecerf, M., Rousselle, P., Perennec, P., & Pelletier, G. (1987) · Plant Science · doi:10.1016/0168-9452(87)90127-0 · Cited by 31 · see citing works
The U6 small nuclear RNA gene family of potato
Guérineau, F., & Waugh, R. (1993) · Plant Molecular Biology · doi:10.1007/bf00027367 · Cited by 13 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Precise plant genome editing using base editors and prime editors
Molla, K. A., Sretenovic, S., Bansal, K. C., & Qi, Y. (2021) · Nature Plants · doi:10.1038/s41477-021-00991-1 · Cited by 387 · see citing works
González, M. N., Massa, G. A., Andersson, M., Turesson, H., Olsson, N., Fält, A., Storani, L., Oneto, C. A. D., Hofvander, P., & Feingold, S. E. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2019.01649 · Cited by 285 · see citing works
Targeted genome-modification tools and their advanced applications in crop breeding
Li, B., Sun, C., Li, J., & Gao, C. (2024) · Nature Reviews Genetics · doi:10.1038/s41576-024-00720-2 · Cited by 259 · see citing works
Application of CRISPR/Cas9 in Crop Quality Improvement
Liu, Q., Yang, F., Zhang, J., Liu, H., Rahman, S., Islam, S., Ma, W., & She, M. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22084206 · Cited by 231 · see citing works
Development of Plant Prime-Editing Systems for Precise Genome Editing
Xu, R., Li, J., Liu, X., Shan, T., Qin, R., & Wei, P. (2020) · Plant Communications · doi:10.1016/j.xplc.2020.100043 · Cited by 209 · see citing works
Can gene editing reduce postharvest waste and loss of fruit, vegetables, and ornamentals?
Shipman, E. N., Yu, J., Zhou, J., Albornoz, K., & Beckles, D. M. (2020) · Horticulture Research · doi:10.1038/s41438-020-00428-4 · Cited by 141 · see citing works
Multiplex genome-editing technologies for revolutionizing plant biology and crop improvement
Abdelrahman, M., Wei, Z., Rohila, J. S., & Zhao, K. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.721203 · Cited by 125 · see citing works
Kumar, D., Yadav, A., Ahmad, R., Dwivedi, U. N., & Yadav, K. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.932859 · Cited by 132 · see citing works
Prime Editing in the model plant Physcomitrium patens and its potential in the tetraploid potato
Perroud, P., Guyon‐Debast, A., Veillet, F., Kermarrec, M.-P., Chauvin, L., Chauvin, J.-É., Gallois, J., & Nogué, F. (2021) · Plant Science · doi:10.1016/j.plantsci.2021.111162 · Cited by 123 · see citing works
Self-citationAdvances in Crop Breeding Through Precision Genome Editing
Nerkar, G., Devarumath, S., Purankar, M. V., Kumar, A., Valarmathi, R., Devarumath, R. M., & Appunu, C. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.880195 · Cited by 121 · see citing works
Genome editing of polyploid crops: prospects, achievements and bottlenecks
Schaart, J. G., van de Wiel, C. C. M., & SMULDERS, M. J. M. (2021) · Transgenic Research · doi:10.1007/s11248-021-00251-0 · Cited by 107 · see citing works
Toward an understanding of potato starch structure, function, biosynthesis, and applications
Tong, C., Ma, Z., Chen, H., & Gao, H. (2023) · Food Frontiers · doi:10.1002/fft2.223 · Cited by 100 · see citing works
Lal, M. K., Tiwari, R. K., Kumar, A., Dey, A., Kumar, R., Kumar, D., Jaiswal, A. K., Changan, S. S., Raigond, P., Dutt, S., et al. (2022) · Plants · doi:10.3390/plants11212857 · Cited by 104 · see citing works
Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World
Hamdan, M. F., Noor, S. N. M., Abd-Aziz, N., Pua, T., & Tan, B. C. (2022) · Plants · doi:10.3390/plants11101297 · Cited by 101 · see citing works
Malabarba, J., Chevreau, E., Dousset, N., Veillet, F., Moizan, J., & Vergne, E. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms22010319 · Cited by 80 · see citing works
Self-citationEvolution of plant mutagenesis tools: a shifting paradigm from random to targeted genome editing
Shelake, R. M., Pramanik, D., & Kim, J. (2019) · Plant Biotechnology Reports · doi:10.1007/s11816-019-00562-z · Cited by 77 · see citing works
Li, Q., Sapkota, M., & van der Knaap, E. (2020) · Horticulture Research · doi:10.1038/s41438-020-0258-8 · Cited by 73 · see citing works
Heritable gene editing using FT mobile guide RNAs and DNA viruses
Lei, J., Peihong, D., Li, Y., Zhang, W., Zhou, G., Liu, C., & Liu, X. (2021) · Plant Methods · doi:10.1186/s13007-021-00719-4 · Cited by 73 · see citing works
Zheng, Z., Ye, G., Zhou, Y., Pu, X., Su, W., & Wang, J. (2021) · All Life · doi:10.1080/26895293.2021.1925358 · Cited by 69 · see citing works
Hegde, N., Joshi, S., Soni, N., & Kushalappa, A. C. (2020) · Plant Cell Reports · doi:10.1007/s00299-020-02629-6 · Cited by 65 · see citing works
Sevestre, F., Facon, M., Wattebled, F., & Szydlowski, N. (2020) · Scientific Reports · doi:10.1038/s41598-020-58985-6 · Cited by 64 · see citing works
Self-citationKumari, C., Sharma, M., Kumar, V., Sharma, R., Kumar, V., Sharma, P., Kumar, P., & Irfan, M. (2022) · Bioengineering · doi:10.3390/bioengineering9040176 · Cited by 60 · see citing works
Veillet, F., Perrot, L., Guyon‐Debast, A., Kermarrec, M.-P., Chauvin, L., Chauvin, J.-É., Gallois, J., Mazier, M., & Nogué, F. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21031024 · Cited by 58 · see citing works
Self-citationCRISPR/Cas systems: opportunities and challenges for crop breeding
Biswas, S., Zhang, D., & Shi, J. (2021) · Plant Cell Reports · doi:10.1007/s00299-021-02708-2 · Cited by 58 · see citing works
CRISPR-induced indels and base editing using the Staphylococcus aureus Cas9 in potato
Veillet, F., Kermarrec, M.-P., Chauvin, L., Chauvin, J.-É., & Nogué, F. (2020) · PLoS ONE · doi:10.1371/journal.pone.0235942 · Cited by 55 · see citing works
Self-citationPrecision Breeding Made Real with CRISPR: Illustration through Genetic Resistance to Pathogens
Veillet, F., Durand, M., Kroj, T., Césari, S., & Gallois, J. (2020) · Plant Communications · doi:10.1016/j.xplc.2020.100102 · Cited by 58 · see citing works
Self-citationAli, Q., Yu, C., Hussain, A., Ali, M., Ahmar, S., Sohail, M. A., Riaz, M., Ashraf, M. F., Abdalmegeed, D., Wang, X., et al. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.860281 · Cited by 50 · see citing works
Potato: from functional genomics to genetic improvement
Li, Q., Huang, X., Su, X., Zhu, G., Zheng, L., Lin, J., Wang, J., & Xue, H. (2024) · Molecular Horticulture · doi:10.1186/s43897-024-00105-3 · Cited by 46 · see citing works
González, M. N., Massa, G. A., Andersson, M., Décima Oneto, C. A., Turesson, H., Storani, L., Olsson, N., Fält, A.-S., Hofvander, P., & Feingold, S. E. (2021) · Plant Cell, Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-020-02008-9 · Cited by 44 · see citing works
CRISPR/Cas Genome Editing in Potato: Current Status and Future Perspectives
Tiwari, J. K., Buckseth, T., Challam, C., Zinta, R., Bhatia, N., Dalamu, D., Naik, S., Poonia, A. K., Singh, R. K., Luthra, S. K., et al. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.827808 · Cited by 44 · see citing works
Base Editors for Citrus Gene Editing
Huang, X., Wang, Y., & Wang, N. (2022) · Frontiers in Genome Editing · doi:10.3389/fgeed.2022.852867 · Cited by 44 · see citing works
Emerging applications of gene editing technologies for the development of climate-resilient crops
Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 43 · see citing works
CRISPR/Cas9: Regulations and challenges for law enforcement to combat its dual-use
Mir, T. U. G., Wani, A. K., Akhtar, N., & Shukla, S. (2022) · Forensic Science International · doi:10.1016/j.forsciint.2022.111274 · Cited by 43 · see citing works
Palanivel, H., Abda, E. M., Shah, S., Prabhu, S. V., Jayakumar, M., Karmegam, N., Kim, W., & Govarthanan, M. (2023) · Journal of Environmental Management · doi:10.1016/j.jenvman.2023.117382 · Cited by 40 · see citing works
Recent advances and challenges in potato improvement using CRISPR/Cas genome editing
Chincinska, I., Miklaszewska, M., & Sołtys‐Kalina, D. (2022) · Planta · doi:10.1007/s00425-022-04054-3 · Cited by 38 · see citing works
Employing CRISPR/Cas Technology for the Improvement of Potato and Other Tuber Crops
TUSSIPKAN, D., & Manabayeva, S. A. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.747476 · Cited by 37 · see citing works
Research progress in gene editing technology
Huang, S., Yan, Y., Su, F., Huang, X., Xia, D., Jiang, X., Dong, Y., Lv, P., Chen, F., & Lv, Y. (2021) · Frontiers in Bioscience-Landmark · doi:10.52586/4997 · Cited by 38 · see citing works
State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential
Nahirñak, V., Almasia, N. I., González, M. N., Massa, G. A., Oneto, C. A. D., Feingold, S. E., Hopp, H. E., & Vazquez-Rovere, C. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2021.768233 · Cited by 34 · see citing works
Genome editing in horticultural crops: augmenting trait development and stress resilience
Sohail, H., Noor, I., Hussain, H., Zhang, L., Xu, X., Chen, X., & Yang, X. (2025) · Horticultural Plant Journal · doi:10.1016/j.hpj.2025.09.001 · Cited by 32 · see citing works
The roles of starch branching enzymes and starch synthase in the biosynthesis of amylose in rice
Okpala, N. E., Aloryi, K. D., An, T., He, L., & Tang, X. (2022) · Journal of Cereal Science · doi:10.1016/j.jcs.2021.103393 · Cited by 29 · see citing works
Blennow, A., Skryhan, K., Tanackovic, V., Krunic, S. L., Shaik, S. S., Andersen, M. S., Kirk, H. G., & Nielsen, K. L. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13367 · Cited by 28 · see citing works
CRISPR/Cas9-mediated fine-tuning of miRNA expression in tetraploid potato
Lukan, T., Veillet, F., Križnik, M., Coll, A., Povalej, T. M., Pogačar, K., Stare, K., Chauvin, L., Chauvin, J.-É., & Gruden, K. (2022) · Horticulture Research · doi:10.1093/hr/uhac147 · Cited by 28 · see citing works
Self-citationModern Plant Biotechnology: An Antidote against Global Food Insecurity
Animasaun, D. A., Adedibu, P. A., Shkryl, Y., Emmanuel, F. O., Tekutyeva, L., & Balabanova, L. (2023) · Agronomy · doi:10.3390/agronomy13082038 · Cited by 31 · see citing works
Guyon‐Debast, A., Alboresi, A., Terret, Z., Charlot, F., Berthier, F., Vendrell‐Mir, P., Casacuberta, J., Veillet, F., Morosinotto, T., Gallois, J., et al. (2021) · New Phytologist · doi:10.1111/nph.17171 · Cited by 27 · see citing works
Self-citationPlant genome editing: CRISPR, base editing, prime editing, and beyond
Xie, Y., Haq, S. I. U., Jiang, X., Zheng, D., Feng, N., Wang, W., He, J., & Qiu, Q. (2022) · Grassland Research · doi:10.1002/glr2.12034 · Cited by 27 · see citing works
Potato trait development going fast-forward with genome editing
Hofvander, P., Andréasson, E., & Andersson, M. (2021) · Trends in Genetics · doi:10.1016/j.tig.2021.10.004 · Cited by 26 · see citing works
The Generic Risks and the Potential of SDN-1 Applications in Crop Plants
Kawall, K. (2021) · Plants · doi:10.3390/plants10112259 · Cited by 28 · see citing works
CRISPR/Cas mediated genome editing in potato: Past achievements and future directions
Tuncel, A., & Qi, Y. (2022) · Plant Science · doi:10.1016/j.plantsci.2022.111474 · Cited by 23 · see citing works
CRISPR/Cas genome editing system and its application in potato
Hou, X., Guo, X., Zhang, Y., & Zhang, Q. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2023.1017388 · Cited by 21 · see citing works
Mohammadi, M. A., Maximiano, M. R., Hosseini, S. M., & Franco, O. L. (2023) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-022-02842-5 · Cited by 20 · see citing works
CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato
Abeuova, L. S., Kali, B. R., TUSSIPKAN, D., Akhmetollayeva, A. S., Ramankulov, Y. M., & Manabayeva, S. A. (2023) · Transgenic Research · doi:10.1007/s11248-023-00356-8 · Cited by 20 · see citing works
Advanced Approaches for Biofortification
Kiran, K. (2020) · Springer eBooks · doi:10.1007/978-981-15-2874-3_2 · Cited by 19 · see citing works
Genome editing advancements in potato (Solanum tuberosum L.): operational challenges and solutions
Mali, S., Dutta, M., & Zinta, G. (2022) · Journal of Plant Biochemistry and Biotechnology · doi:10.1007/s13562-022-00812-2 · Cited by 18 · see citing works
CRISPR/Cas9: an advanced platform for root and tuber crops improvement
Divya, K., Thangaraj, M., & Radhika, N. K. (2024) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1242510 · Cited by 18 · see citing works
Roychowdhury, R., Das, S. P., Das, S., Biswas, S., Patel, M. K., Kumar, A., Sarker, U., Pal, S., Das, R., Yogendra, K. N., et al. (2025) · Functional & Integrative Genomics · doi:10.1007/s10142-025-01533-0 · Cited by 18 · see citing works
Advances and Applications of Plant Base Editing Technologies
Peng, H. F., Li, J., Sun, K., Tang, H., Huang, W., Li, X., Wang, S., Ding, K., Han, Z., Li, Z., et al. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26199452 · Cited by 17 · see citing works
European genome editing regulations: threats to the European bioeconomy and unfit for purpose
Hjort, C., Cole, J. A., & Frébort, I. (2021) · EFB Bioeconomy Journal · doi:10.1016/j.bioeco.2021.100001 · Cited by 16 · see citing works
Modified Gene Editing Systems: Diverse Bioengineering Tools and Crop Improvement
Zhu, G., & Zhu, H. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.847169 · Cited by 16 · see citing works
Adoption of CRISPR-Cas for crop production: present status and future prospects
Akanmu, A. O., Asemoloye, M. D., Marchisio, M. A., & Babalola, O. O. (2024) · PeerJ · doi:10.7717/peerj.17402 · Cited by 14 · see citing works
Advanced Genome Editing Technologies: Potentials and Prospects in Improvement of Sugar crops
Amaresh, G., Nunavath, A., Appunu, C., Viswanathan, C., KUMAR, R. R., Gujjar, R. S., & Manimekalai, R. (2024) · Sugar Tech · doi:10.1007/s12355-024-01447-4 · Cited by 14 · see citing works
Comprehending the evolution of gene editing platforms for crop trait improvement
Dhakate, P., Sehgal, D., Vaishnavi, S., Chandra, A., Singh, A., Raina, S. N., & Rajpal, V. R. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.876987 · Cited by 14 · see citing works
Tailoring crops with superior product quality through genome editing: an update
Ravikiran, K. T., Thribhuvan, R., Sheoran, S., Kumar, S., Kushwaha, A. K., Vineeth, T. V., & Saini, M. (2023) · Planta · doi:10.1007/s00425-023-04112-4 · Cited by 14 · see citing works
Kushwaha, S. B., Nagesh, C. R., Lele, S. S., Viswanathan, C., Prashat, G. R., Goswami, S., Kumar, R. R., Kunchge, N. S., Gokhale, J. S., & Vinutha, T. (2025) · Scientia Horticulturae · doi:10.1016/j.scienta.2025.113957 · Cited by 11 · see citing works
Improvement of Crops Using the CRISPR/Cas System: New Target Genes
Ukhatova, Y., Erastenkova, M. V., Korshikova, E. S., Krylova, E. A., Mikhailova, A. S., Semilet, T. V., Tikhonova, N. G., Shvachko, N. A., & Хлесткина, Е. К. (2023) · Molecular Biology · doi:10.31857/s0026898423030151 · Cited by 11 · see citing works
Improvement of Crops Using the CRISPR/Cas System: New Target Genes
Ukhatova, Y. V., Erastenkova, M. V., Korshikova, E. S., Krylova, E. A., Mikhailova, A. S., Semilet, T. V., Tikhonova, N. G., Shvachko, N. A., & Хлесткина, Е. К. (2023) · Molecular Biology · doi:10.1134/s0026893323030135 · Cited by 11 · see citing works
Gene Editing in Potato Using CRISPR-Cas9 Technology
Chauvin, L., Sevestre, F., Lukan, T., Nogué, F., Gallois, J., Chauvin, J.-É., & Veillet, F. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1609-3_16 · Cited by 10 · see citing works
Self-citationCRISPR-Cas Gene Editing Technology in Potato
Сапахова, З., Канат, Р., Choi, K., Дауров, Д., Даурова, А., Жамбакин, К., & Шамекова, М. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26157496 · Cited by 9 · see citing works
Cytosine base editors optimized for genome editing in potato protoplasts
Westberg, I. N., Carlsen, F. M., Johansen, I. E., & Petersen, B. L. (2023) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1247702 · Cited by 10 · see citing works
Sharma, V., Jahan, K., Kumar, P., Puri, A., Sharma, V. K., Mishra, A., Bharatam, P. V., Sharma, D., Rishi, V., & Roy, J. K. (2022) · Functional & Integrative Genomics · doi:10.1007/s10142-022-00923-y · Cited by 9 · see citing works
Хромов, А. В., Makhotenko, A. V., Makarova, S. S., Suprunova, T. P., Kalinina, N. O., & Taliansky, M. E. (2020) · Russian Journal of Bioorganic Chemistry · doi:10.1134/s1068162020060138 · Cited by 8 · see citing works
CRISPR/Cas-Mediated Genome Editing for Improving Key Traits in Potato (Solanum tuberosum L.)
Sood, S., Dipta, B., Mangal, V., Thakur, A. K., Dutt, S., Sharma, N., Kumar, V. D., & Singh, B. (2024) · Journal of Plant Growth Regulation · doi:10.1007/s00344-024-11514-5 · Cited by 7 · see citing works
CRISPR/Cas9 Technology for Potato Functional Genomics and Breeding
González, M. N., Massa, G. A., Andersson, M., Storani, L., Olsson, N., Oneto, C. A. D., Hofvander, P., & Feingold, S. E. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-3131-7_21 · Cited by 7 · see citing works
Kumari, N., Kumar, A., Sharma, S., Thakur, P., Chadha, S., & Dhiman, A. (2024) · Journal of Plant Biochemistry and Biotechnology · doi:10.1007/s13562-024-00878-0 · Cited by 6 · see citing works
Crop plants with improved culture and quality traits for food, feed and other uses
Nogué, F., Vergne, P., Chèvre, A., Chauvin, J.-É., Bouchabké‐Coussa, O., Déjardin, A., Chevreau, E., Oyant, L. H., Mazier, M., Barret, P., et al. (2019) · Transgenic Research · doi:10.1007/s11248-019-00135-4 · Cited by 6 · see citing works
Self-citationFrom Lab to Field: CRISPRing Major Cultivated Solanaceae for Crop Improvement
Martina, F., Acquadro, A., & Moglia, A. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27052238 · Cited by 5 · see citing works
Plant Mutagenesis Tools for Precision Breeding: Conventional CRISPR/Cas9 Tools and Beyond
Shelake, R. M., Pramanik, D., Waghunde, R. R., & Kim, J. (2023) · Springer eBooks · doi:10.1007/978-981-16-9720-3_10 · Cited by 6 · see citing works
Development and optimization of base editors and its application in crops
Jiang, C., Li, Y., Wang, R., Sun, X., Zhang, Y., & Zhang, Q. (2024) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2024.150942 · Cited by 6 · see citing works
Starch biosynthesis and crop bioengineering
Blennow, A., Hebelstrup, K. H., & Petersen, B. L. (2025) · Grain & Oil Science and Technology · doi:10.1016/j.gaost.2025.04.003 · Cited by 4 · see citing works
Emerging trends in the development of efficient CAS nucleases for meticulous gene editing in plants
Gujjar, R. S., Sherin, S., Batra, S., Mahto, A., Goswami, S. K., KUMAR, R. R., Tiwari, R. K., Singh, A., & Kumari, M. (2025) · Plant Science · doi:10.1016/j.plantsci.2025.112820 · Cited by 4 · see citing works
Wakasa, Y., Kasai, A., Yamazaki, M., Tabei, Y., Tsuyama, M., Igarashi, T., Okazaki, T., Yamamoto, K., Fujihara, H., Kanno, A., et al. (2020) · Plant Cell Reports · doi:10.1007/s00299-020-02572-6 · Cited by 4 · see citing works
Nezhdanova, A. V., Efremov, G. I., Slugina, M. A., Kamionskaya, A. M., Kochieva, E. Z., & Shchennikova, A. V. (2022) · Horticulturae · doi:10.3390/horticulturae8080730 · Cited by 4 · see citing works
The United States Potato Genebank Holding of cv. Desiree is a Somatic Mutant of cv. Urgenta
Amundson, K., Henry, I. M., & Comai, L. (2022) · American Journal of Potato Research · doi:10.1007/s12230-022-09892-1 · Cited by 4 · see citing works
Prospects for Genome Editing of Potato
Dev, S. S., Joseph, J., & Rosario, L. L. D. (2021) · IntechOpen eBooks · doi:10.5772/intechopen.99278 · Cited by 3 · see citing works
Sankhla, I. S., Kumar, A., & Singh, C. P. (2024) · Springer eBooks · doi:10.1007/978-981-99-8529-6_12 · Cited by 3 · see citing works
Genome Editing for Trait Improvement in Potato (Solanum tuberosum L.)
Mali, S., & Zinta, G. (2024) · Springer eBooks · doi:10.1007/978-981-97-3119-0_16 · Cited by 3 · see citing works
Recent Trends in Genome Editing Technologies for Agricultural Crop Improvement
Ahmad, H. M., Iqbal, M. S., Abdullah, M., El‐Tabakh, M. A. M., Oranab, S., Mudassar, M., Shimira, F., & Zahid, G. (2023) · Springer eBooks · doi:10.1007/978-3-031-15568-0_17 · Cited by 3 · see citing works
Transgenic and genome editing approaches for starch modification in potato- a review
Batta, S., Sharma, N., Gupta, R., Singh, B., & Thakur, A. K. (2026) · Plant Gene · doi:10.1016/j.plgene.2026.100585 · Cited by 2 · see citing works
Overcoming breeding barriers with genome editing in autopolyploid crops
Enciso-Rodríguez, F. E., Barrero, L. S., Garzón-Martínez, G. A., Kim, J. H., Kumam, Y., Pagliai, F. A., Jiang, T., Huo, H., & Muńoz, P. (2026) · BMC Plant Biology · doi:10.1186/s12870-026-08862-7 · Cited by 2 · see citing works
The transcription factor StC3H14 enhances cold tolerance through the CBF-dependent pathway in potato
Sun, S., Geng, H., Sina, Q., Fu, H., Wu, Y., Yang, R., Kong, W., Wang, Y., & Huang, B. (2026) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiag450 · Cited by 2 · see citing works
Fernández, R., Lizana, C. N. R., & Behn, A. (2022) · Agro Sur · doi:10.4206/agrosur.2022.v50n2-01 · Cited by 2 · see citing works
Role of genetic engineering in improving potato production
Saeed, F., Dangol, S. D., Hashmi, M. H., Hossain, M. J., & Bakhsh, A. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-822925-5.00006-2 · Cited by 2 · see citing works
CRISPR-Cas and Its Applications in Food Production
Rai, N., Singh, N. A., Goyal, J., Upadhayay, P., & Jain, V. (2024) · Springer eBooks · doi:10.1007/978-981-99-8529-6_13 · Cited by 2 · see citing works
Commercialization of CRISPR-edited crops: Opportunities and challenges
Saxena, S., Sushmita, L. C., Babu, Y. M., Chavan, D. D., Venu, E., Mandal, S., Hurrah, I. M., Mohture, V. M., & Mishra, T. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-13229-2.00031-4 · Cited by 3 · see citing works
CRISPR: A Promising Toolbox for Better Crops
Sharma, N., Batta, S., Bhagta, S., Pathania, S., Dipta, B., Kumar, A., Singh, B., Siddappa, S., Sood, S., & Bhardwaj, V. (2024) · Springer eBooks · doi:10.1007/978-981-97-1223-6_2 · Cited by 2 · see citing works
Sun, S., Guan, B., Xing, Y., Li, X., Liu, L., Li, Y., Jia, L., Ye, S., Dossa, K., Li, Z., et al. (2024) · BMC Plant Biology · doi:10.1186/s12870-024-05122-4 · Cited by 1 · see citing works
Genome Editing in Potato Using CRISPR/Cas Technology: Applications and Challenges
Beniwal, D., Chauhan, S., & Dhillon, H. K. (2024) · Genetics. · doi:10.5772/intechopen.1003940 · Cited by 1 · see citing works
CRISPR/Cas: A precise and advanced tool for quality trait improvement in crops
Kumar, R., Singh, S., GANGWAR, L. K., Kumar, V., Ojre, A., Singh, A., Yadav, N., Kaur, N., Puri, P., Negi, R., et al. (2025) · Ecological Genetics and Genomics · doi:10.1016/j.egg.2025.100346 · Cited by 1 · see citing works
CRISPR/Cas system and AI tools for effective gene editing in vegetable crops: a systematic review
Kumar, M., Chaudhary, V., Chaudhary, V., Kumar, R., Sirohi, U., Singh, R., Singh, D., & Teotia, S. (2026) · Discover Applied Sciences · doi:10.1007/s42452-026-08305-w · Cited by 1 · see citing works
Advancements in genome editing tools and technologies for improvement of horticultural crops
Sivasankarreddy, K., Pandey, V., Pandey, S., Singh, S. K., & Patil, B. L. (2026) · Journal of Plant Biochemistry and Biotechnology · doi:10.1007/s13562-026-01079-7 · Cited by 1 · see citing works
Kang, X., Gao, W., Cheng, Y., Cui, B., Qiao, X., & El‐Aty, A. M. A. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.3c07576 · Cited by 1 · see citing works
- Authors
- Veillet, F., Chauvin, L., Kermarrec, M.-P., Sevestre, F., Merrer, M., Terret, Z., Szydlowski, N., Devaux, P., Gallois, J.-L., & Chauvin, J.-E.
- Affiliations
- Institut Agro Rennes-Angers (FR)
- Centre National de la Recherche Scientifique (FR)
- Génétique et amélioration des fruits et légumes (FR)
- Corresponding author
- Veillet, Florian
- ORCID
- Venue
- Plant Cell Reports
- Published
- 2019-05-17
- Volume
- 38
- Issue
- 9
- Pages
- 1065-1080
- ISSN
- 0721-7714 (ISSN-L)
- 1432-203X
- OpenAlex
- W2942972964
- Cited by
- 124
- References
- 79
- Citations by year
- 2026: 10 · 2025: 10 · 2024: 21 · 2023: 16 · 2022: 25 · 2021: 23 · 2020: 16 · 2019: 3
- FWCI
- 5.55
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- Plant Cell Reports (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- LillOA (Université de Lille (University Of Lille)) (submittedVersion)
- LillOA (Université de Lille (University Of Lille)) (submittedVersion, other-oa)
- Funders
- Agence Nationale de la Recherche (ANR-11-BTBR-0001_GENIUS)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Keywords
- tetraploid potato, potato, CRISPR/Cas9 base editing, amylose biosynthesis, starch synthase, loss-of-function mutants, high-resolution melting analysis, genome editing, molecular breeding
- MeSH terms
- Gene Editing, Alleles, Solanum tuberosum, Starch Synthase, Tetraploidy, CRISPR-Cas Systems
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
