Consumption of Bifidobacterium animalis subsp. lactis BB-12 in yogurt reduced expression of TLR-2 on peripheral blood-derived monocytes and pro-inflammatory cytokine secretion in young adults
Abstract
Purpose Probiotic bacteria modulate immune parameters and inflammatory outcomes. Emerging evidence demonstrates that the matrix used to deliver probiotics may influence the efficacy of probiotic interventions in vivo. The aims of the current study were to evaluate (1) the effect of one species, Bifidobacterium animalis subsp. lactis BB-12 at a dose of log10 ± 0.5 CFUs/day on immune responses in a randomized, partially blinded, 4-period crossover, free-living study, and (2) whether the immune response to BB-12 differed depending on the delivery matrix.Methods Healthy adults (n = 30) aged 18-40 years were recruited and received four treatments in a random order: (A) yogurt smoothie alone; smoothie with BB-12 added (B) before or (C) after yogurt fermentation, or (D) BB-12 given in capsule form. At baseline and after each 4-week treatment, peripheral blood mononuclear cells (PBMCs) were isolated, and functional and phenotypic marker expression was assessed.Results BB-12 interacted with peripheral myeloid cells via Toll-like receptor 2 (TLR-2). The percentage of CD14+HLA-DR+ cells in peripheral blood was increased in male participants by all yogurt-containing treatments compared to baseline (p = 0.0356). Participants who consumed yogurt smoothie with BB-12 added post-fermentation had significantly lower expression of TLR-2 on CD14+HLA-DR+ cells (p = 0.0186) and reduction in TNF-α secretion from BB-12- (p = 0.0490) or LPS-stimulated (p = 0.0387) PBMCs compared to baseline.Conclusions These findings not only demonstrate a potential anti-inflammatory effect of BB-12 in healthy adults, but also indicate that the delivery matrix influences the immunomodulatory properties of BB-12.
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The physiological functions of Bifidobacterium include restraining the growth of pathogenic bacteria, inhibiting proinflammatory cytokines, protecting against intestinal barrier dysfunction (in vitro and in vivo), synthesizing vitamins and amino acids in the intestine, increasing calcium bioavailability, and antitumor effects.
“The physiological functions of Bifidobacterium are mainly as follows: restraining the growth of pathogenic bacteria, inhibiting proinflammatory cytokines [26,27], protecting against intestinal barrier dysfunction (in vitro and in vivo), synthesizing vitamins and amino acids in the intestine and increasing calcium bioavailability [28,29], and antitumor effects, according to experimental results sug”
From The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties (2024) · cites this paper as [28]
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The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties
Maftei, N.-M., Raileanu, C. R., Balta, A. A., Ambrose, L., Boev, M., Marin, D. B., & Lisa, E. L. (2024) · Microorganisms · doi:10.3390/microorganisms12020234 · Cited by 381 · see citing works
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- Authors
- Meng, H., Ba, Z., Lee, Y., Peng, J., Lin, J., Fleming, J. A., Furumoto, E. J., Roberts, R. F., Kris-Etherton, P. M., & Rogers, C. J.
- Affiliations
- Pennsylvania State University (US)
- Corresponding author
- Rogers, Connie J.
- ORCID
- Venue
- European Journal of Nutrition
- Published
- 2015-11-30
- Volume
- 56
- Issue
- 2
- Pages
- 649-661
- ISSN
- 1436-6207 (ISSN-L)
- 1436-6215
- OpenAlex
- W2269538796
- Cited by
- 52
- References
- 36
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 9 · 2023: 9 · 2022: 7 · 2021: 9 · 2020: 2 · 2019: 2 · 2018: 3 · 2017: 5 · 2016: 2
- FWCI
- 4.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- European Journal of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Dairy Research Institute
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Keywords
- Bifidobacterium animalis subsp. lactis BB-12, probiotics, yogurt, TLR2, monocytes, TNF-α, inflammatory cytokines, anti-inflammatory activity, peripheral blood mononuclear cells, immunomodulation
- MeSH terms
- Bifidobacterium animalis, Adult, Fermentation, HLA-DR Antigens, Humans, Immunity, Inflammation, Leukocytes, Mononuclear, Lipopolysaccharides, Tumor Necrosis Factor-alpha, Yogurt, Cytokines, Lipopolysaccharide Receptors, Probiotics, Toll-Like Receptor 2, Young Adult
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