Determination of stratum corneum lipid profile by tape stripping in combination with high-performance thin-layer chromatography
Abstract
Intercellular lipids in the stratum corneum (SC) are responsible for the barrier function of mammalian skin. The main components of the SC lipids are ceramides, cholesterol, and free fatty acids, as established by thin-layer chromatographic analysis of lipids extracted from the human and mammalian SC. Up to now, for lipid analysis the extracts of the entire SC has been used and information on whether the lipid composition changes with the depth in the SC is scarce. Tape stripping is a technique which removes corneocyte layers step by step with an adhesive film. The use of this technique for lipid analysis was hampered by the contamination of lipid extracts with compounds co-extracted from the tape with organic solvents used for the extraction of SC lipids. The aim of the present study was to establish a suitable analytical method for the determination of the local SC lipid composition. For this purpose, the SC samples were collected by sequential stripping with Leukoplex tape in five healthy volunteers. The lipids were extracted with ethyl acetate:methanol mixture (20:80) and separated by means of HPTLC. The results of this study revealed that the free fatty acid level is highest and the cholesterol and ceramide levels lowest in the uppermost SC layers (about 4 strippings). The levels remained unchanged in the underlying SC layers. In these layers, the ceramide level was about 60 wt% and the free fatty acid and cholesterol levels were about 20 wt% each. Ceramides could be sep…
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The composition of ceramide classes/species in the human stratum corneum has previously been analyzed using LC/MS.
“The composition of ceramide classes/species in the human SC has previously been analyzed using LC/MS (5-7, 16, 17, 29, 30).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [30]
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- Authors
- Weerheim, A., & Ponec, M.
- Affiliations
- Leiden University Medical Center (NL)
- Venue
- Archives of Dermatological Research
- Published
- 2001-04-24
- Volume
- 293
- Issue
- 4
- Pages
- 191-199
- ISSN
- 0003-9187 (ISSN-L)
- 0340-3696
- 1432-069X
- OpenAlex
- W2026520510
- Cited by
- 302
- References
- 36
- Citations by year
- 2026: 8 · 2025: 15 · 2024: 13 · 2023: 9 · 2022: 12 · 2021: 16 · 2020: 20 · 2019: 11 · 2018: 20 · 2017: 18 · 2016: 18 · 2015: 15 · 2014: 11 · 2013: 17 · 2012: 10
- FWCI
- 7.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Archives of Dermatological Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Stiftung Forschung 3R
- H2020 European Research Council
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
- Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
- Keywords
- stratum corneum lipids, tape stripping, ceramide, cholesterol, free fatty acids, cholesterol sulfate, skin barrier function
- MeSH terms
- Ceramides, Cholesterol, Cholesterol Esters, Chromatography, High Pressure Liquid, Epidermis, Fatty Acids, Nonesterified, Humans, Methods, Tissue Distribution, Lipid Metabolism
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