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Cited by 10Probiotics and Fermented FoodsInfant Nutrition and HealthGut microbiota and health

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Genome analysis and 2’-fucosyllactose utilization characteristics of a new Akkermansia muciniphila strain isolated from mice feces

Gao, Wei, Xiao, Mengshi, Gu, Ziqiang, Fu, Xiaodan, Ren, Xinmiao, Yu, Ying, Liu, Zhemin, Zhu, Changliang, Kong, Qing, Mou, Haijin·2022·Molecular Genetics and Genomics
DOI 10.1007/s00438-022-01937-8
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Abstract

Akkermansia muciniphila is considered to be a next-generation probiotic, and closely related to host metabolism and immune response. Compared with other probiotics, little is known about its genomic analysis. Therefore, further researches about isolating more A. muciniphila strains and exploring functional genes are needed. In the present study, a new strain isolated from mice feces was identified as A. muciniphila (Muc X). Whole-genome sequencing and annotation revealed that Muc X possesses key genes necessary for human milk oligosaccharides (HMO) utilization, including α-L-fucosidases, β-galactosidases, exo-α-sialidases, and β-acetylhexosaminidases. The complete metabolic pathways for γ-aminobutyric acid and squalene and genes encoding functional proteins, such as the outer membrane protein Amuc_1100, were annotated in the Muc X genome. Comparative genome analysis was used to identify functional genes unique to Muc X compared to six other A. muciniphila strains. Results showed Muc X genome possesses unique genes, including sugar transporters and transferases. Single-strain incubation revealed faster utilization of 2'-fucosyllactose (2'-FL), galacto-oligosaccharides, and lactose by Muc X than by A. muciniphila DSM 22959. This study isolated and identified an A. muciniphila strain that can utilize 2'-FL, and expolored the genes related to HMO utilization and special metabolites, which provided a theoretical basis for the further excavation of A. muciniphila function and the compound application with fucosylated oligosaccharides.

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Metadata

Authors
Gao, W., Xiao, M., Gu, Z., Fu, X., Ren, X., Yu, Y., Liu, Z., Zhu, C., Kong, Q., & Mou, H.
Affiliations
  • Ocean University of China (CN)
  • Nanchang University (CN)
Corresponding author
Mou, Haijin
ORCID
Venue
Molecular Genetics and Genomics
Publisher
Springer Science and Business Media LLC
Published
2022-08-10
Volume
297
Issue
6
Pages
1515-1528
ISSN
  • 1617-4615 (ISSN-L)
  • 1617-4623
DOI
10.1007/s00438-022-01937-8
OpenAlex
W4290960081
Cited by
10
References
6
Citations by year
2026: 1 · 2025: 5 · 2024: 1 · 2023: 3
FWCI
1.20
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Molecular Genetics and Genomics (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (31872893)
  • China Postdoctoral Science Foundation (2021M701547)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
Akkermansia muciniphila, human milk oligosaccharides, gut microbiota, probiotics, whole genome sequencing, comparative genomics, sugar transporters, galactooligosaccharides, lactose metabolism, Amuc_1100
MeSH terms
Akkermansia, Animals, beta-Galactosidase, Feces, gamma-Aminobutyric Acid, Humans, Lactose, Membrane Proteins, Oligosaccharides, Squalene, Transferases, Trisaccharides, Mice, Verrucomicrobia

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