Correlation of oxidant status with oxidative tissue damage in patients with rheumatoid arthritis
Abstract
Rheumatoid arthritis (RA) is a debilitating autoimmune disease whose etiology remains unknown, but studies have consistently implicated a plethora of inflammatory mechanisms culminating in chronic symmetric and erosive synovitis. Importantly, reactive oxygen species (ROS) have been attributed to directly contribute towards the destructive, proliferative synovitis evident in RA. Accordingly, this study aimed to establish whether the degree of oxidative stress and disease activity score (DAS28) correlated with the downstream effects of oxidative damage. The redox status of neutrophils sourced from synovial fluid (SF) was measured by flow cytometry in terms of total ROS and hydroxyl radicals. Among the molecular damage markers, protein carbonylation and lipid peroxidation were detected by spectrophotometry and S-nitrosothiols by fluorimetry. Neutrophils constituted the major cellular component of the SF of patients with RA and their levels of ROS and hydroxyl radicals correlated strongly with protein carbonylation and lipid peroxidation. However, all the oxidative damage markers correlated positively with DAS28. Taken together, in patients with RA, the strong correlation between levels of ROS and DAS28 with markers of oxidative damage suggests that measurement of oxidative stress could serve as a biomarker for monitoring disease severity in RA.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In higher organisms, NO is involved in cell signaling and maintenance of homeostasis, but high levels of this metabolite lead to chemical stress.
“In higher organisms, NO is involved in cell signaling and maintenance of homeostasis, but high levels of this metabolite lead to chemical stress, possibly also contributing to the induction and progression of diseases [38,39].”
From Known Antimicrobials Versus Nortriptyline in Candida albicans: Repositioning an Old Drug for New Targets (2020) · cites this paper as [38]
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
Arnett, F. C., Edworthy, S. M., Bloch, D. A., McShane, D. J., Fries, J. F., Cooper, N. S., Healey, L. A., Kaplan, S. R., Liang, M. H., Luthra, H. S., et al. (1988) · Arthritis & Rheumatism · doi:10.1002/art.1780310302 · Cited by 19,753 · see citing works
Free radicals in biology and medicine
Gutteridge, J. M. C. (1991) · General Pharmacology The Vascular System · doi:10.1016/0306-3623(91)90478-o · Cited by 17,178 · see citing works
[30] Microsomal lipid peroxidation
Buege, J. A., & Aust, S. D. (1978) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(78)52032-6 · Cited by 13,433 · see citing works
Free Radicals in Biology and Medicine
Halliwell, B., & Gutteridge, J. M. C. (2015) · doi:10.1093/acprof:oso/9780198717478.001.0001 · Cited by 8,990 · see citing works
Revised Criteria for the Classification of Rheumatoid Arthritis
Arnett, F. C. (1990) · Orthopaedic Nursing · doi:10.1097/00006416-199003000-00011 · Cited by 7,736 · see citing works
[49] Determination of carbonyl content in oxidatively modified proteins
Levine, R. L., Garland, D. L., Oliver, C. N., Amici, A., Climent, I., Lenz, A.-G., Ahn, B. W., Shaltiel, S., & Stadtman, E. R. (1990) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(90)86141-h · Cited by 5,895 · see citing works
Biochemistry of nitric oxide and its redox-activated forms
Stamler, J. S., Singel, D. J., & Loscalzo, J. (1992) · Science · doi:10.1126/science.1281928 · Cited by 2,744 · see citing works
Biomarkers of Oxidative Damage in Human Disease
Dalle-Donne, I., Rossi, R., Colombo, R., Giustarini, D., & Milzani, A. (2006) · Clinical Chemistry · doi:10.1373/clinchem.2005.061408 · Cited by 1,703 · see citing works
Protein carbonylation in human diseases
Dalle-Donne, I., Giustarini, D., Colombo, R., Rossi, R., & Milzani, A. (2003) · Trends in Molecular Medicine · doi:10.1016/s1471-4914(03)00031-5 · Cited by 961 · see citing works
Forman, H., Fukuto, J., & Torres, M. (2004) · American Journal of Physiology - Cell Physiology · doi:10.1152/ajpcell.00516.2003 · Cited by 538 · see citing works
Lipid peroxidation and tissue damage.
Mylonas, C. C., & Kouretas, D. (1999) · PubMed · Cited by 431 · see citing works
Marzinzig, M., Nussler, A. K., Stadler, J., Marzinzig, E., Barthlen, W., Nüssler, N. C., Beger, H. G., Morris, S. M., & Brückner, U. B. (1997) · Nitric Oxide · doi:10.1006/niox.1997.0116 · Cited by 331 · see citing works
Redox signalling and the inflammatory response in rheumatoid arthritis
Filippin, L. I., Vercelino, R., Marroni, N. P., & Xavier, R. M. (2008) · Clinical & Experimental Immunology · doi:10.1111/j.1365-2249.2008.03634.x · Cited by 328 · see citing works
Hormonal, environmental, and infectious risk factors for developing systemic lupus erythematosus
Cooper, G. S., Dooley, M. A., Treadwell, E. L., Clair, E. W. S., Parks, C. G., & Gilkeson, G. S. (1998) · Arthritis & Rheumatism · doi:10.1002/1529-0131(199810)41:10 · Cited by 221 · see citing works
Kundu, S., Ghosh, P., Datta, S., Ghosh, A., Chattopadhyay, S., & Chatterjee, M. (2012) · Free Radical Research · doi:10.3109/10715762.2012.727991 · Cited by 188 · see citing works
Self-citationLipid, protein, DNA oxidation and antioxidant status in rheumatoid arthritis
Seven, A., Güzel, S., Aslan, M., & Hamuryudan, V. (2008) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2008.01.029 · Cited by 168 · see citing works
The role of reactive oxygen species in immunopathogenesis of rheumatoid arthritis.
Mirshafiey, A., & Mohsenzadegan, M. (2008) · PubMed · Cited by 158 · see citing works
Mandal, G., Wyllie, S., Singh, N., Sundar, S., Fairlamb, A. H., & Chatterjee, M. (2007) · Parasitology · doi:10.1017/s0031182007003150 · Cited by 110 · see citing works
Self-citationBanji, D., Pinnapureddy, J., Banji, O. J. F., Saidulu, A., & Hayath, M. S. (2011) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2011.06.006 · Cited by 92 · see citing works
Bansal, S., Chawla, D., Siddarth, M., Banerjee, B. D., Madhu, S. V., & Tripathi, A. K. (2012) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2012.10.019 · Cited by 83 · see citing works
Serum Antioxidative Enzymes Levels and Oxidative Stress Products in Age-Related Cataract Patients
Chang, D., Zhang, X., Rong, S., Sha, Q., Liu, P., Han, T., & Pan, H. (2013) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2013/587826 · Cited by 82 · see citing works
Testosterone suppresses oxidative stress in human neutrophils
Marin, D. P., Bolin, A. P., dos Santos, R. D. C. M., et al. (2010) · Cell Biochemistry and Function · doi:10.1002/cbf.1669 · Cited by 82 · see citing works
Mantle, D., Falkous, G., & Walker, D. (1999) · Clinica Chimica Acta · doi:10.1016/s0009-8981(99)00055-8 · Cited by 72 · see citing works
Guilpain, P., Chéreau, C., Goulvestre, C., Servettaz, A., Montani, D., Tamas, N., Pagnoux, C., HACHULLA, E., Weill, B. J., Guillevin, L., et al. (2010) · European Respiratory Journal · doi:10.1183/09031936.00148409 · Cited by 65 · see citing works
Oxidative stress parameters in different systemic rheumatic diseases
Firuzi, O., Fuksa, L., Spadaro, C., Boušová, I., Riccieri, V., Spadaro, A., Petrucci, R., Marrosu, G., & Saso, L. (2006) · Journal of Pharmacy and Pharmacology · doi:10.1211/jpp.58.7.0010 · Cited by 64 · see citing works
Kundu, S., Bala, A., Ghosh, P., et al. (2011) · Free Radical Research · doi:10.3109/10715762.2011.555480 · Cited by 59 · see citing works
Self-citationIncreased Serum Oxidative Stress Markers in Women with Uterine Leiomyoma
Santulli, P., Borghese, B., Lemaréchal, H., Leconte, M., Millischer, A., Batteux, F., Chapron, C., & Borderie, D. (2013) · PLoS ONE · doi:10.1371/journal.pone.0072069 · Cited by 51 · see citing works
van der Linden, M. P. M., Batstra, M. R., Bakker‐Jonges, L. E., Detert, J., Bastian, H., Scherer, H. U., Toes, R. E. M., Burmester, G. R., Mjaavatten, M. D., Kvien, T. K., et al. (2010) · Arthritis & Rheumatism · doi:10.1002/art.30200 · Cited by 50 · see citing works
Bhattacharya, S., Mula, S., Gamre, S. S., Kamat, J. P., Bandyopadhyay, S. K., & Chattopadhyay, S. (2006) · Food Chemistry · doi:10.1016/j.foodchem.2005.12.041 · Cited by 38 · see citing works
Dhiman, M., Coronado, Y. A., Vallejo, C. K., et al. (2013) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0002364 · Cited by 36 · see citing works
Roy, S., Sannigrahi, S., Vaddepalli, R. P., et al. (2012) · Inflammation · doi:10.1007/s10753-012-9457-2 · Cited by 31 · see citing works
Dráfi, F., Bauerová, K., Kuncírová, V., et al. (2012) · Interdisciplinary Toxicology · doi:10.2478/v10102-012-0015-4 · Cited by 20 · see citing works
Yang, X., Liu, X., Shang, J., et al. (2013) · PubMed · Cited by 8 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Ali, E. A. I., Barakat, B. M., & Hassan, R. (2015) · PLOS ONE · doi:10.1371/journal.pone.0121523 · Cited by 73 · see citing works
Arleevskaya, M. I., Shafigullina, A. Z., Filina, Y. V., Lemerle, J., & Renaudineau, Y. (2017) · Frontiers in Immunology · doi:10.3389/fimmu.2017.01725 · Cited by 24 · see citing works
Caldara, M., & Marmiroli, N. (2020) · Microorganisms · doi:10.3390/microorganisms8050742 · Cited by 8 · see citing works
- Authors
- Datta, S., Kundu, S., Ghosh, P., De, S., Ghosh, A., & Chatterjee, M.
- Affiliations
- Institute of Post Graduate Medical Education and Research (IN)
- Corresponding author
- Chatterjee, Mitali
- Venue
- Clinical Rheumatology
- Published
- 2014-04-10
- Volume
- 33
- Issue
- 11
- Pages
- 1557-1564
- ISSN
- 0770-3198 (ISSN-L)
- 1434-9949
- OpenAlex
- W2116951100
- Cited by
- 123
- References
- 34
- Citations by year
- 2026: 7 · 2025: 7 · 2024: 13 · 2023: 7 · 2022: 20 · 2021: 9 · 2020: 5 · 2019: 9 · 2018: 12 · 2017: 12 · 2016: 11 · 2015: 8 · 2014: 2
- FWCI
- 7.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Clinical Rheumatology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- rheumatoid arthritis, oxidative stress, reactive oxygen species, hydroxyl radicals, neutrophils, synovial fluid, protein carbonylation, lipid peroxidation, S-nitrosothiols, oxidative damage biomarkers, disease severity monitoring
- MeSH terms
- Adult, Arthritis, Rheumatoid, Female, Humans, Male, Middle Aged, Neutrophils, Oxidation-Reduction, Synovial Fluid, Synovitis, Lipid Peroxidation, Biomarkers, Reactive Oxygen Species, Oxidative Stress, Young Adult
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
