Cited by 22Cervical Cancer and HPV ResearchReproductive tract infections researchWomen's cancer prevention and management

Anyplex II HPV28 detection and Anyplex II HPV HR detection assays are highly concordant with other commercial assays for detection of high-risk HPV genotypes in women with high grade cervical abnormalities

Cornall, A. M., Poljak, M., Garland, S. M., Phillips, S., Tan, J. H., Machalek, D. A., Quinn, M. A., Tabrizi, S. N.·2017·European Journal of Clinical Microbiology & Infectious Diseases

Abstract

Purpose to evaluate the performance of Anyplex II HPV28 and HPV HR Detection assays against the EuroArray HPV, Cobas 4800 HPV (Cobas), HPV Amplicor (Amp), Linear Array HPV (LA) and Hybrid Capture 2 (HC2) in detection of high-risk HPV (HR-HPV) from liquid-based cervical cytology samples. Methods cervical specimens from 404 women undergoing management of high-grade cytological abnormality were evaluated by Anyplex II HPV28 and HPV HR Detection assays for detection of HR-HPV genotypes and prediction of histologically-confirmed cervical intraepithelial neoplasia grade 2 or higher (≥CIN2). The results were compared to EuroArray, HC2, Cobas, Amp, and LA. Results specimens were evaluated from 404 women with an average age of 30 years, including 336 with a histological diagnosis of ≥ CIN2 and 68 with ≤ CIN1. Concordance of HR-HPV detection between Anyplex II HPV28 and other genotyping assays was 94.79 % (κ = 0.84; EuroArray) and 97.27 % (κ = 0.91; LA); and between Anyplex II HPV HR and other HR-HPV detection assays was 86.35 % (κ = 0.62; HC2), 96.03 % (κ = 0.87; Cobas) and 96.77 % (κ = 0.89; Amp). Using HR-HPV detection for prediction of ≥ CIN2 by Anyplex II HPV28 and HPV HR, sensitivity (90.18, 95 % CI 86.48-93.14; 90.77, 95 % CI 87.16-93.65) and specificity (both 67.16, 95 % CI 54.60-78.15) were not significantly different to the other HPV assays tested, with one exception. Both Anyplex assays had significantly higher sensitivity than HC2 (p 0.05) of HC2 in this high-risk populatio…

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Crossref · 10.1007/s10096-016-2831-5

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Authors
Cornall, A. M., Poljak, M., Garland, S. M., Phillips, S., Tan, J. H., Machalek, D. A., Quinn, M. A., & Tabrizi, S. N.
Affiliations
Royal Women's Hospital (AU)
Corresponding author
Cornall, A. M.
Venue
European Journal of Clinical Microbiology & Infectious Diseases
Published
2016-11-08
Volume
36
Issue
3
Pages
545-551
ISSN
  • 0934-9723 (ISSN-L)
  • 1435-4373
OpenAlex
W2551892186
Cited by
22
References
14
Citations by year
2025: 2 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 1 · 2019: 2 · 2018: 7 · 2017: 2 · 2016: 1
FWCI
3.75
Type
journal article
Language
en
Open access
closed
Locations
  • European Journal of Clinical Microbiology & Infectious Diseases (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Women's cancer prevention and management
Keywords
high-risk HPV genotyping, high-grade cervical intraepithelial neoplasia, liquid-based cervical cytology, Hybrid Capture 2, assay sensitivity and specificity
MeSH terms
Adult, Uterine Cervical Dysplasia, Female, Genotype, Humans, Sensitivity and Specificity, Molecular Diagnostic Techniques, Papillomaviridae, Papillomavirus Infections

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