Anyplex II HPV28 detection and Anyplex II HPV HR detection assays are highly concordant with other commercial assays for detection of high-risk HPV genotypes in women with high grade cervical abnormalities
Abstract
Purpose to evaluate the performance of Anyplex II HPV28 and HPV HR Detection assays against the EuroArray HPV, Cobas 4800 HPV (Cobas), HPV Amplicor (Amp), Linear Array HPV (LA) and Hybrid Capture 2 (HC2) in detection of high-risk HPV (HR-HPV) from liquid-based cervical cytology samples. Methods cervical specimens from 404 women undergoing management of high-grade cytological abnormality were evaluated by Anyplex II HPV28 and HPV HR Detection assays for detection of HR-HPV genotypes and prediction of histologically-confirmed cervical intraepithelial neoplasia grade 2 or higher (≥CIN2). The results were compared to EuroArray, HC2, Cobas, Amp, and LA. Results specimens were evaluated from 404 women with an average age of 30 years, including 336 with a histological diagnosis of ≥ CIN2 and 68 with ≤ CIN1. Concordance of HR-HPV detection between Anyplex II HPV28 and other genotyping assays was 94.79 % (κ = 0.84; EuroArray) and 97.27 % (κ = 0.91; LA); and between Anyplex II HPV HR and other HR-HPV detection assays was 86.35 % (κ = 0.62; HC2), 96.03 % (κ = 0.87; Cobas) and 96.77 % (κ = 0.89; Amp). Using HR-HPV detection for prediction of ≥ CIN2 by Anyplex II HPV28 and HPV HR, sensitivity (90.18, 95 % CI 86.48-93.14; 90.77, 95 % CI 87.16-93.65) and specificity (both 67.16, 95 % CI 54.60-78.15) were not significantly different to the other HPV assays tested, with one exception. Both Anyplex assays had significantly higher sensitivity than HC2 (p 0.05) of HC2 in this high-risk populatio…
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AnyplexTM II demonstrated similar sensitivity to LA for most high-risk genotypes, with slightly lower sensitivity observed for HPV51 or 52.
“Additionally, the authors reported that Anyplex™ II demonstrated similar sensitivity to LA for most high-risk genotypes, with slightly lower sensitivity observed for HPV51 or 52 [14, 32].”
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EVALUATION OF A HUMAN PAPILLOMAVIRUS GENOTYPING ASSAY FOR CERVICAL CANCER SCREENING IN TANZANIA
kapie, K. (2018) · Lincoln (University of Nebraska)
- Authors
- Cornall, A. M., Poljak, M., Garland, S. M., Phillips, S., Tan, J. H., Machalek, D. A., Quinn, M. A., & Tabrizi, S. N.
- Affiliations
- Royal Women's Hospital (AU)
- Corresponding author
- Cornall, A. M.
- ORCID
- Venue
- European Journal of Clinical Microbiology & Infectious Diseases
- Published
- 2016-11-08
- Volume
- 36
- Issue
- 3
- Pages
- 545-551
- ISSN
- 0934-9723 (ISSN-L)
- 1435-4373
- OpenAlex
- W2551892186
- Cited by
- 22
- References
- 14
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 1 · 2019: 2 · 2018: 7 · 2017: 2 · 2016: 1
- FWCI
- 3.75
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Clinical Microbiology & Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Women's cancer prevention and management
- Keywords
- high-risk HPV genotyping, high-grade cervical intraepithelial neoplasia, liquid-based cervical cytology, Hybrid Capture 2, assay sensitivity and specificity
- MeSH terms
- Adult, Uterine Cervical Dysplasia, Female, Genotype, Humans, Sensitivity and Specificity, Molecular Diagnostic Techniques, Papillomaviridae, Papillomavirus Infections
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