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Cryopreservation of Whole Rat Livers by Vitrification and Nanowarming
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“So far, we and others have published on nanowarming of volumes up to 80 mL CPA mL and 30 mL biological nanowarming at rates up to 50–100 °C/min14–19 (see Table S1).”
From Physical vitrification and nanowarming at liter-scale CPA volumes: toward organ cryopreservation (2025) · cites this paper as [19]
In the citing paper
“We found that CPA damage, not physical injury from vitrification and nanowarming, was the limiting step for biologic and functional recovery.”
From Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model (2023) · cites this paper as [19]
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“In the past, we and others have shown that nanowarming can rewarm vitrified organs (including kidneys) from animal models with physical success, but only partial biological recovery and no transplant data17–20.”
From Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model (2023) · cites this paper as [19]
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Research works printed but not linked: 46
- Allen, J. W., T. Hassanein, and S. N. Bhatia. Advances in bioartificial liver devices. Hepatology. 34:447–455, 2001. · DOI
- Arav, A., O. Friedman, Y. Natan, E. Gur, and N. Shani. Rat hindlimb cryopreservation and transplantation: a step toward “organ banking.” Am. J. Transplant. 17:2820–2828, 2017. · DOI
- Arav, A., Z. Gavish, A. Elami, Y. Natan, A. Revel, S. Silber, R. G. Gosden, and P. Patrizio. Ovarian function 6 years after cryopreservation and transplantation of whole sheep ovaries. Reprod. Biomed. Online. 20:48–52, 2010. · DOI
- Bald, W. B. On crystal size and cooling rate. J. Microsc. 143:89–102, 1986. · DOI
- Berendsen, T. A., B. G. Bruinsma, C. F. Puts, N. Saeidi, O. B. Usta, B. E. Uygun, M. L. Izamis, M. Toner, M. L. Yarmush, and K. Uygun. Supercooling enables long-term transplantation survival following 4 days of liver preservation. Nat. Med. 20:790–793, 2014. · DOI
- Bischof, J. C. Quantitative measurement and prediction of biophysical response during freezing in tissues. Annu. Rev. Biomed. Eng. 2:257–288, 2000. · DOI
- Broelsch, C. E., J. C. Emond, P. F. Whitington, J. R. Thistlethwaite, A. L. Baker, and J. L. Lichtor. Application of reduced-size liver transplants as split grafts, auxiliary orthotopic grafts, and living related segmental transplants. Ann. Surg. 212:368–375, 1990. · DOI
- Ceresa, C. D. L., D. Nasralla, S. Knight, and P. J. Friend. Cold storage or normothermic perfusion for liver transplantation: probable application and indications. Curr Opin Organ Transplant. 22:300–305, 2017. · DOI
- Chiu-Lam, A., E. Staples, C. J. Pepine, and C. Rinaldi. Perfusion, cryopreservation, and nanowarming of whole hearts using colloidally stable magnetic cryopreservation agent solutions. Sci. Adv. 7:10, 2021. · DOI · Paper page
- Collins, G. M., M. Bravo-Shugarman, and P. I. Terasaki. Kidney preservation for transportation. Initial perfusion and 30 hours’ ice storage. Lancet. 2:1219–1222, 1969. · DOI
- de Graaf, I. A., A. L. Draaisma, O. Schoeman, G. M. Fahy, G. M. Groothuis, and H. J. Koster. Cryopreservation of rat precision-cut liver and kidney slices by rapid freezing and vitrification. Cryobiology. 54:1–12, 2007. · DOI
- de Vries, R. J., S. N. Tessier, P. D. Banik, S. Nagpal, S. E. J. Cronin, S. Ozer, E. O. A. Hafiz, T. M. van Gulik, M. L. Yarmush, J. F. Markmann, M. Toner, H. Yeh, and K. Uygun. Supercooling extends preservation time of human livers. Nat. Biotechnol. 37:1131–1136, 2019. · DOI
- Elliott, G. D., S. Wang, and B. J. Fuller. Cryoprotectants: a review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures. Cryobiology. 76:74–91, 2017. · DOI · Paper page
- Etheridge, M. L., Y. Xu, L. Rott, J. Choi, B. Glasmacher, and J. C. Bischof. RF heating of magnetic nanoparticles improves the thawing of cryopreserved biomaterials. Technology (Singap. World Sci.). 02:229–242, 2014.
- Fahy, G. M., N. Guan, I. A. de Graaf, Y. Tan, L. Griffin, and G. M. Groothuis. Cryopreservation of precision-cut tissue slices. Xenobiotica. 43:113–132, 2013. · DOI
- Fahy, G. M., D. R. MacFarlane, C. A. Angell, and H. T. Meryman. Vitrification as an approach to cryopreservation. Cryobiology. 21:407–426, 1984. · DOI · Paper page
- Fahy, G. M., B. Wowk, R. Pagotan, A. Chang, J. Phan, B. Thomson, and L. Phan. Physical and biological aspects of renal vitrification. Organogenesis. 5:167–175, 2009. · DOI · Paper page
- Forbes, S. J., S. Gupta, and A. Dhawan. Cell therapy for liver disease: from liver transplantation to cell factory. J. Hepatol. 62:S157-169, 2015. · DOI
- Gao, Z., B. Namsrai, Z. Han, P. Joshi, J. S. Rao, V. Ravikumar, A. Sharma, H. L. Ring, D. Idiyatullin, E. C. Magnuson, P. A. Iaizzo, E. G. Tolkacheva, M. Garwood, Y. Rabin, M. Etheridge, E. B. Finger, and J. C. Bischof. Vitrification and rewarming of magnetic nanoparticle-loaded rat hearts. Adv. Mater. Technol. 7:2100873, 2022. · DOI · Paper page
- Gao, Z., H. L. Ring, A. Sharma, B. Namsrai, N. Tran, E. B. Finger, M. Garwood, C. L. Haynes, and J. C. Bischof. Preparation of scalable silica-coated iron oxide nanoparticles for nanowarming. Adv. Sci. (Weinh). 7:1901624, 2020. · DOI · Paper page
- Gavish, Z., M. Ben-Haim, and A. Arav. Cryopreservation of whole murine and porcine livers. Rejuvenation Res. 11:765–772, 2008. · DOI · Paper page
- Guan, N., S. A. Blomsma, P. M. van Midwoud, G. M. Fahy, G. M. Groothuis, and I. A. de Graaf. Effects of cryoprotectant addition and washout methods on the viability of precision-cut liver slices. Cryobiology. 65:179–187, 2012. · DOI
- Han, Z., and J. C. Bischof. Critical cooling and warming rates as a function of CPA concentration. CryoLetters. 41:185–193, 2020.
- Han, Z., A. Sharma, Z. Gao, T. W. Carlson, M. G. O’Sullivan, E. B. Finger, and J. C. Bischof. Diffusion limited cryopreservation of tissue with radiofrequency heated metal forms. Adv. Healthc. Mater. 9:e2000796, 2020. · DOI
- Iansante, V., A. Chandrashekran, and A. Dhawan. Cell-based liver therapies: past, present and future. Philos. Trans. R. Soc. Lond. B Biol. Sci. 373:20170229, 2018. · DOI
- KarowJr., A. M. Cryoprotectants: a new class of drugs. J. Pharm. Pharmacol. 21:209–223, 1969. · DOI · Paper page
- Klassen, D. K., L. B. Edwards, D. E. Stewart, A. K. Glazier, J. P. Orlowski, and C. L. Berg. The OPTN deceased donor potential study: implications for policy and practice. Am. J. Transplant. 16:1707–1714, 2016. · DOI
- Kuleshova, L. L., X. W. Wang, Y. N. Wu, Y. Zhou, and H. Yu. Vitrification of encapsulated hepatocytes with reduced cooling and warming rates. Cryo Lett. 25:241–254, 2004.
- Li, A. P. In vitro human hepatocyte-based experimental systems for the evaluation of human drug metabolism, drug-drug interactions, and drug toxicity in drug development. Curr. Top. Med. Chem. 14:1325–1338, 2014. · DOI
- Luyet, B. J. The vitrification of organic colloids and of protoplasm. Biodynamica. 1:1–14, 1937.
- MacConmara, M., S. I. Hanish, C. S. Hwang, L. De Gregorio, D. M. Desai, C. A. Feizpour, B. Tanriover, J. F. Markmann, H. Zeh 3rd., and P. A. Vagefi. Making every liver count: increased transplant yield of donor livers through normothermic machine perfusion. Ann. Surg. 272:397–401, 2020. · DOI
- Magalhaes, R., X. W. Wang, S. S. Gouk, K. H. Lee, C. M. Ten, H. Yu, and L. L. Kuleshova. Vitrification successfully preserves hepatocyte spheroids. Cell Transplant. 17:813–828, 2008. · DOI
- Manuchehrabadi, N., Z. Gao, J. Zhang, H. L. Ring, Q. Shao, F. Liu, M. McDermott, A. Fok, Y. Rabin, K. G. Brockbank, M. Garwood, C. L. Haynes, and J. C. Bischof. Improved tissue cryopreservation using inductive heating of magnetic nanoparticles. Sci. Transl. Med. 9:10, 2017. · DOI · Paper page
- Mazur, P. Freezing of living cells: mechanisms and implications. Am. J. Physiol. 247:C125-142, 1984. · DOI · Paper page
- McKenzie, T. J., J. B. Lillegard, and S. L. Nyberg. Artificial and bioartificial liver support. Semin. Liver Dis. 28:210–217, 2008. · DOI
- Nasralla, D., C. C. Coussios, H. Mergental, M. Z. Akhtar, A. J. Butler, C. D. L. Ceresa, V. Chiocchia, S. J. Dutton, J. C. García-Valdecasas, N. Heaton, C. Imber, W. Jassem, I. Jochmans, J. Karani, S. R. Knight, P. Kocabayoglu, M. Malagò, D. Mirza, P. J. Morris, A. Pallan, A. Paul, M. Pavel, M. Perera, J. Pirenne, R. Ravikumar, L. Russell, S. Upponi, C. J. E. Watson, A. Weissenbacher, R. J. Ploeg, and P. J. Friend. A randomized trial of normothermic preservation in liver transplantation. Nature. 557:50–56, 2018. · DOI · Paper page
- Pegg, D. E. Principles of cryopreservation. Methods Mol. Biol. 368:39–57, 2007. · DOI
- Plitz, J., Y. Rabin, and J. R. Walsh. The effect of thermal expansion of ingredients on the cocktails VS55 and DP6. Cell Preserv. Technol. 2:215–226, 2004. · DOI
- Revel, A., A. Elami, A. Bor, S. Yavin, Y. Natan, and A. Arav. Whole sheep ovary cryopreservation and transplantation. Fertil. Steril. 82:1714–1715, 2004. · DOI
- Sharma, A., J. S. Rao, Z. Han, L. Gangwar, B. Namsrai, Z. Gao, H. L. Ring, E. Magnuson, M. Etheridge, B. Wowk, G. M. Fahy, M. Garwood, E. B. Finger, and J. C. Bischof. Vitrification and nanowarming of kidneys. Adv. Sci. (Weinh). 8:e2101691, 2021. · DOI · Paper page
- Tessier, S. N., R. J. de Vries, C. A. Pendexter, S. E. J. Cronin, S. Ozer, E. O. A. Hafiz, S. Raigani, J. P. Oliveira-Costa, B. T. Wilks, M. Lopera Higuita, T. M. van Gulik, O. B. Usta, S. L. Stott, H. Yeh, M. L. Yarmush, K. Uygun, and M. Toner. Partial freezing of rat livers extends preservation time by 5-fold. Nat. Commun. 13:4008, 2022. · DOI
- van Rijn, R., I. J. Schurink, Y. de Vries, A. P. van den Berg, M. Cortes Cerisuelo, S. Darwish Murad, J. I. Erdmann, N. Gilbo, R. J. de Haas, N. Heaton, B. van Hoek, V. A. L. Huurman, I. Jochmans, O. B. van Leeuwen, V. E. de Meijer, D. Monbaliu, W. G. Polak, J. J. G. Slangen, R. I. Troisi, A. van lander, J. de Jonge, and R. J. Porte. Hypothermic machine perfusion in liver transplantation—a randomized trial. N. Engl. J. Med. 384:1391–1401, 2021. · DOI
- Vinken, M., and J. G. Hengstler. Characterization of hepatocyte-based in vitro systems for reliable toxicity testing. Arch. Toxicol. 92:2981–2986, 2018. · DOI
- Xu, H., C. Y. Lee, M. G. Clemens, and J. X. Zhang. Pronlonged hypothermic machine perfusion preserves hepatocellular function but potentiates endothelial cell dysfunction in rat livers. Transplantation. 77:1676–1682, 2004. · DOI
- Zhan, L., J. S. Rao, N. Sethia, M. Q. Slama, Z. Han, D. Tobolt, M. Etheridge, Q. P. Peterson, C. S. Dutcher, J. C. Bischof, and E. B. Finger. Pancreatic islet cryopreservation by vitrification achieves high viability, function, recovery and clinical scalability for transplantation. Nat. Med. 28:798–808, 2022. · DOI · Paper page
- Zhang, J., H. L. Ring, K. R. Hurley, Q. Shao, C. S. Carlson, D. Idiyatullin, N. Manuchehrabadi, P. J. Hoopes, C. L. Haynes, J. C. Bischof, and M. Garwood. Quantification and biodistribution of iron oxide nanoparticles in the primary clearance organs of mice using T1 contrast for heating. Magn. Reson. Med. 78:702–712, 2017. · DOI
Cited by
Sorted by citations
Han, Z., Rao, J. S., Gangwar, L., Namsrai, B.-E., Pasek-Allen, J. L., Etheridge, M. L., Wolf, S. M., Pruett, T. L., Bischof, J. C., & Finger, E. B. (2023) · Nature Communications · doi:10.1038/s41467-023-38824-8 · Cited by 173
Self-citationPhysical vitrification and nanowarming at liter-scale CPA volumes: toward organ cryopreservation
Gangwar, L., Han, Z., Scheithauer, C., Namsrai, B.-E., Kantesaria, S., Goldstein, R., Etheridge, M. L., Finger, E. B., & Bischof, J. C. (2025) · Nature Communications · doi:10.1038/s41467-025-63483-2 · Cited by 26
Self-citation
Metadata
- Authors
- Sharma, A., Lee, C. Y., Namsrai, B.-E., Han, Z., Tobolt, D., Rao, J. S., Gao, Z., Etheridge, M. L., Garwood, M., Clemens, M. G., Bischof, J. C., & Finger, E. B.
- ORCID
- Finger, Erik B.
- Venue
- Annals of Biomedical Engineering
- Published
- 2022-10-01
- Volume
- 51
- Issue
- 3
- Pages
- 566-577
- ISSN
- 0090-6964
- 1573-9686
- PMC
- PMC10315167
- Cited by
- 62
- References
- 0
- Language
- en
- Update notices
- correction (2022-11-29)
- Licence
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (DK117425, DK131209, DK126551)
- National Heart, Lung, and Blood Institute (HL135046)
- National Science Foundation (EEC 1941543)
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