A study of fucoidan from the brown seaweed Chorda filum
Abstract
Fucoidan fractions from the brown seaweed Chorda filum were studied using solvolytic desulfation. Methylation analysis and NMR spectroscopy were applied for native and desulfated polysaccharides. Homofucan sulfate from C. filum was shown to contain poly-alpha-(1-->3)-fucopyranoside backbone with a high degree of branching, mainly of alpha-(1-->2)-linked single units. Some fucopyranose residues are sulfated at O-4 (mainly) and O-2 positions. Some alpha-(1-->3)-linked fucose residues were shown by NMR to be 2-O-acetylated. The 1H and 13C NMR spectra of desulfated, deacetylated fucan were completely assigned. The spectral data obtained correspond to a quasiregular polysaccharide structure with a branched hexasaccharide repeating unit. Other fucoidan fractions from C. filum have more complex carbohydrate composition and give rather complex methylation patterns. [formula: see text]
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Chorda filum fucoidan fractions have an α-(1-3)-linked backbone with multiple branching and a majority of α-(1-2)-linked monomers, with sulfation mainly at position 4 and rarely at position 2.
“Structural analysis revealed the presence of an α-(1-3)-linked backbone with multiple branching and a majority of α-(1-2)-linked monomers. Sulfation was mainly observed in position 4 and rarely in position 2.”
From A Review on Fucoidan Structure, Extraction Techniques, and Its Role as an Immunomodulatory Agent (2022) · cites this paper as [24]
FCSPs isolated from Chorda filum consist of a poly-α(1→3)-fucopyranose backbone with a high degree of branching mainly as α(1→2)-linked single α-l-fucopyranosyl residues.
“FCSPs isolated from Chorda filum (Laminariales) have been shown to consist of a poly-α(1→3)-fucopyranose backbone with a high degree of branching mainly as α(1→2)-linked single α-l-fucopyranosyl residues (Figure 4) [24,25];”
Fucoidans from Chorda filum consist of a core backbone structure of poly-α(1→3)-linked L-fucopyranoses sulfated mainly at C-4, sometimes at C-2, with some α(1→3)-linked fucose residues being 2-O-acetylated.
“These structural units are similar to those found in the fucoidans from Chorda filum (Laminariales) which consist of a core backbone structure of poly-a(1A3)-linked L-fucopyranoses sulfated mainly at C-4, sometimes at the C-2 position, and with some of the a(1A3)linked fucose residues being 2-O-acetylated.4,14”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [4]
Classical extraction of fucoidans may include calcium addition to promote alginate precipitation during purification.
“and may include calcium addition to promote alginate precipitation during purification, 3,4”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [4]
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- Authors
- Chizhov, A. O., Dell, A., Morris, H. R., Haslam, S. M., McDowell, R. A., Shashkov, A. S., Nifant’ev, N. E., Khatuntseva, E. A., & Usov, A. I.
- Affiliations
- Russian Academy of Sciences (RU)
- Imperial College London (GB)
- Corresponding author
- Usov, Anatolii I.
- Venue
- Carbohydrate Research
- Publisher
- Elsevier BV
- Published
- 1999-07
- Volume
- 320
- Issue
- 1-2
- Pages
- 108-119
- ISSN
- 0008-6215 (ISSN-L)
- OpenAlex
- W2016691557
- Cited by
- 326
- References
- 30
- Citations by year
- 2026: 6 · 2025: 11 · 2024: 6 · 2023: 10 · 2022: 13 · 2021: 15 · 2020: 20 · 2019: 10 · 2018: 19 · 2017: 18 · 2016: 18 · 2015: 21 · 2014: 18 · 2013: 16 · 2012: 15
- FWCI
- 8.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carbohydrate Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Keywords
- fucoidan, Chorda filum, brown algae, fucan, polysaccharide structure, sulfated polysaccharides, desulfation, methylation analysis, NMR resonance assignment, hexasaccharide repeating unit, polysaccharide composition
- MeSH terms
- Phaeophyceae, Carbohydrate Conformation, Carbohydrate Sequence, Indicators and Reagents, Molecular Sequence Data, Molecular Weight, Oligosaccharides, Polysaccharides, Seaweed, Nuclear Magnetic Resonance, Biomolecular
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