Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device
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This paper describes a simple reversible hydrogel patterning method for 3D cell culture. Alginate gel is formed in select regions of a microfluidic device through light-triggered release of caged calcium. In the pre-gelled alginate solution, calcium is chelated by DM-nitrophen (DM-n) to prevent cross-linking of alginate. After sufficient UV exposure the caged calcium is released from DM-n causing alginate to cross-link. The effect of using different concentrations of calcium and chelating agents as well as the duration of UV exposure is described. Since the cross-linking is based on calcium concentration, the cross-linked alginate can easily be dissolved by EDTA. We also demonstrate application of this capability to patterned microscale 3D co-culture using endothelial cells and osteoblastic cells in a microchannel.
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Biopolymers from non-mammal tissues such as alginate and chitosan are also used for making 3D scaffolds.
“Biopolymers from non-mammal tissues, such as alginate and chitosan, are also used for making 3D scaffolds (23,63,68,84).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [23]
The Introduction describes alginate as a naturally derived polysaccharide, and the Conclusion reports using alginate hydrogels for in vitro 3D cell culture. This supports alginate as a 3D scaffold material, but the paper does not identify its source as non-mammalian or address chitosan. (LLM, conf=0.86)
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- Authors
- Chueh, B.-H., Zheng, Y., Torisawa, Y.-S., Hsiao, A. Y., Ge, C., Hsiong, S., Huebsch, N., Franceschi, R., Mooney, D. J., & Takayama, S.
- Affiliations
- University of Michigan (US)
- Harvard University (US)
- Corresponding author
- Takayama, Shuichi
- ORCID
- Venue
- Biomedical Microdevices
- Published
- 2009-10-15
- Volume
- 12
- Issue
- 1
- Pages
- 145-151
- ISSN
- 1387-2176 (ISSN-L)
- 1572-8781
- PMC
- PMC2825700
- OpenAlex
- W2043956077
- Cited by
- 82
- References
- 43
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 7 · 2020: 5 · 2019: 4 · 2018: 4 · 2017: 7 · 2016: 7 · 2015: 7 · 2014: 9 · 2013: 5 · 2012: 4
- FWCI
- 2.40
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Biomedical Microdevices (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Human Genome Research Institute (R01HG004653, HG004653)
- National Institute of General Medical Sciences (T32GM145304)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- alginate hydrogel, hydrogel patterning, caged calcium, photochemical cross-linking, microfluidics, 3D cell culture, co-culture, endothelial cells, osteoblasts, UV exposure, microchannels
- MeSH terms
- Alginates, Animals, Calcium, Cells, Cultured, Equipment Design, Hexuronic Acids, Humans, Light, Flow Injection Analysis, Cell Culture Techniques, Equipment Failure Analysis, Hydrogels, Glucuronic Acid, Endothelial Cells, Microfluidic Analytical Techniques, Cell Proliferation, Mice
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