Cited by 823D Printing in Biomedical ResearchHydrogels: synthesis, properties, applicationsMicrofluidic and Capillary Electrophoresis Applications

Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device

Open access
Chueh, Bor-han, Zheng, Ying, Torisawa, Yu-suke, Hsiao, Amy Y., Ge, Chunxi, Hsiong, Susan, Huebsch, Nathaniel, Franceschi, Renny +2 more·2010·Biomedical Microdevices

Abstract

This paper describes a simple reversible hydrogel patterning method for 3D cell culture. Alginate gel is formed in select regions of a microfluidic device through light-triggered release of caged calcium. In the pre-gelled alginate solution, calcium is chelated by DM-nitrophen (DM-n) to prevent cross-linking of alginate. After sufficient UV exposure the caged calcium is released from DM-n causing alginate to cross-link. The effect of using different concentrations of calcium and chelating agents as well as the duration of UV exposure is described. Since the cross-linking is based on calcium concentration, the cross-linked alginate can easily be dissolved by EDTA. We also demonstrate application of this capability to patterned microscale 3D co-culture using endothelial cells and osteoblastic cells in a microchannel.

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  • Biopolymers from non-mammal tissues such as alginate and chitosan are also used for making 3D scaffolds.

    “Biopolymers from non-mammal tissues, such as alginate and chitosan, are also used for making 3D scaffolds (23,63,68,84).”

    From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [23]

    The Introduction describes alginate as a naturally derived polysaccharide, and the Conclusion reports using alginate hydrogels for in vitro 3D cell culture. This supports alginate as a 3D scaffold material, but the paper does not identify its source as non-mammalian or address chitosan. (LLM, conf=0.86)

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Research works printed but not linked: 3

  1. Ellis-Davis GCR Nat. Methods 2007 4 619 628 17664946 PMC4207253
  2. Ellis-Davis GCR Meth Enzymol 2003 360A 226 238 12622152
  3. Fishbach C Kong HJ Hsiong SX Evangelista MB Yuen W Mooney DJ Proc. Natl. Acad. Sci. U.S.A 2009 106 399 404 19126683 PMC2626714

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Authors
Chueh, B.-H., Zheng, Y., Torisawa, Y.-S., Hsiao, A. Y., Ge, C., Hsiong, S., Huebsch, N., Franceschi, R., Mooney, D. J., & Takayama, S.
Affiliations
  • University of Michigan (US)
  • Harvard University (US)
Corresponding author
Takayama, Shuichi
Venue
Biomedical Microdevices
Published
2009-10-15
Volume
12
Issue
1
Pages
145-151
ISSN
  • 1387-2176 (ISSN-L)
  • 1572-8781
OpenAlex
W2043956077
Cited by
82
References
43
Citations by year
2026: 3 · 2025: 1 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 7 · 2020: 5 · 2019: 4 · 2018: 4 · 2017: 7 · 2016: 7 · 2015: 7 · 2014: 9 · 2013: 5 · 2012: 4
FWCI
2.40
Type
journal article
Language
en
Open access
Locations
  • Biomedical Microdevices (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Human Genome Research Institute (R01HG004653, HG004653)
  • National Institute of General Medical Sciences (T32GM145304)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Keywords
alginate hydrogel, hydrogel patterning, caged calcium, photochemical cross-linking, microfluidics, 3D cell culture, co-culture, endothelial cells, osteoblasts, UV exposure, microchannels
MeSH terms
Alginates, Animals, Calcium, Cells, Cultured, Equipment Design, Hexuronic Acids, Humans, Light, Flow Injection Analysis, Cell Culture Techniques, Equipment Failure Analysis, Hydrogels, Glucuronic Acid, Endothelial Cells, Microfluidic Analytical Techniques, Cell Proliferation, Mice

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