Retinal pigment epithelium and photoreceptor preconditioning protection requires docosanoid signaling
Open accessAbstract
Omega-3 and omega-6 polyunsaturated fatty acids (PUFAs) are necessary for functional cell integrity. Preconditioning (PC), as we define it, is an acquired protection or resilience by a cell, tissue, or organ to a lethal stimulus enabled by a previous sublethal stressor or stimulus. In this study, we provide evidence that the omega-3 fatty acid docosahexaenoic acid (DHA) and its derivatives, the docosanoids 17-hydroxy docosahexaenoic acid (17-HDHA) and neuroprotectin D1 (NPD1), facilitate cell survival in both in vitro and in vivo models of retinal PC. We also demonstrate that PC requires the enzyme 15-lipoxygenase-1 (15-LOX-1), which synthesizes 17-HDHA and NPD1, and that this is specific to docosanoid signaling despite the concomitant release of the omega-6 arachidonic acid and eicosanoid synthesis. These findings advocate that DHA and docosanoids are protective enablers of PC in photoreceptor and retinal pigment epithelial cells.
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Phospholipase A2 (PLA2), which cleaves the fatty acyl moiety from the number 2 position of phospholipids, is of particular relevance in maintaining RPE homeostasis.
“Phospholipase A2 (PLA2), the enzyme that cleaves the fatty acyl moiety from the number 2 position of phospholipids is of particular relevance in maintaining RPE homeostasis (Bazan, 2018; Knott et al., 2018).”
From The cell biology of the retinal pigment epithelium (2020) · cites this paper as Knott et al., 2018
The protective signaling demonstrated by preconditioning is specific to docosanoid signaling, despite the concomitant release of omega-6 arachidonic acid and eicosanoid synthesis.
“The protective signaling demonstrated by preconditioning is specific to docosanoid signaling, despite the concomitant release of the omega-6 AA and eicosanoid synthesis (Knott et al., 2018).”
From Docosanoids and elovanoids from omega-3 fatty acids are pro-homeostatic modulators of inflammatory responses, cell damage and neuroprotection (2018) · cites this paper as Knott et al., 2018
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Protein kinase C and lipid signaling for sustained cellular responses
Nishizuka, Y. (1995) · The FASEB Journal · doi:10.1096/fasebj.9.7.7737456 · Cited by 2,499 · see citing works
Mukherjee, P. K., Marcheselli, V. L., Serhan, C. N., & Bazan, N. G. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402531101 · Cited by 753 · see citing works
Bazan, N. G., Molina, M. F., & Gordon, W. C. (2011) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.012809.104635 · Cited by 441 · see citing works
Self-citationCell survival matters: docosahexaenoic acid signaling, neuroprotection and photoreceptors
Bazan, N. G. (2006) · Trends in Neurosciences · doi:10.1016/j.tins.2006.03.005 · Cited by 353 · see citing works
Self-citationZhao, Y., Calon, F., Julien, C., Winkler, J. W., Petasis, N. A., Lukiw, W. J., & Bazan, N. G. (2011) · PLoS ONE · doi:10.1371/journal.pone.0015816 · Cited by 243 · see citing works
Self-citationNovel Metabolism of Docosahexaenoic Acid in Neural Cells
Kim, H. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.r700015200 · Cited by 219 · see citing works
Astarita, G., Jung, K.-M., Berchtold, N. C., Nguyen, V. Q., Gillen, D. L., Head, E., Cotman, C. W., & Piomelli, D. (2010) · PLoS ONE · doi:10.1371/journal.pone.0012538 · Cited by 218 · see citing works
Phospholipases A2 and Inflammatory Responses in the Central Nervous System
Sun, G. Y., Shelat, P. B., Jensen, M. B., He, Y., Sun, A. Y., & Simonyi, Á. (2009) · NeuroMolecular Medicine · doi:10.1007/s12017-009-8092-z · Cited by 197 · see citing works
Belayev, L., Khoutorova, L., Atkins, K. D., & Bazan, N. G. (2009) · Stroke · doi:10.1161/strokeaha.109.555979 · Cited by 170 · see citing works
Self-citationLiu, C., Peng, M., Laties, A. M., & Wen, R. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-04-01337.1998 · Cited by 168 · see citing works
(1998) · PubMed · Cited by 158 · see citing works
Bazan, N. G., Calandria, J. M., & Serhan, C. N. (2010) · Journal of Lipid Research · doi:10.1194/jlr.r001131 · Cited by 140 · see citing works
Mechanisms of Ischemic Preconditioning in Rat Hearts
Hu, K., & Nattel, S. (1995) · Circulation · doi:10.1161/01.cir.92.8.2259 · Cited by 129 · see citing works
Bazan, N. G., Musto, A. E., & Knott, E. J. (2011) · Molecular Neurobiology · doi:10.1007/s12035-011-8200-6 · Cited by 125 · see citing works
Self-citationBazan, N. G. (2007) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.07-0918 · Cited by 116 · see citing works
Self-citationMukherjee, P. K., Marcheselli, V. L., Vaccari, J. C. D. R., Gordon, W. C., Jackson, F. R., & Bazan, N. G. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0705963104 · Cited by 110 · see citing works
Self-citationTherapeutic prospects for PEDF: more than a promising angiogenesis inhibitor
Tombran‐Tink, J., & Barnstable, C. J. (2003) · Trends in Molecular Medicine · doi:10.1016/s1471-4914(03)00074-1 · Cited by 109 · see citing works
Docosahexaenoic acid (22:6, n-3) is metabolized to lipoxygenase reaction products in the retina
Bazan, N. G., Birkle, D. L., & Reddy, T. S. (1984) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(84)90601-6 · Cited by 107 · see citing works
Self-citationCalandria, J. M., Marcheselli, V. L., Mukherjee, P. K., Uddin, J., Winkler, J. W., Petasis, N. A., & Bazan, N. G. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.003988 · Cited by 104 · see citing works
Henke, D. C., Kouzan, S., & Eling, T. E. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90137-4 · Cited by 103 · see citing works
Faghiri, Z., & Bazan, N. G. (2010) · Experimental Eye Research · doi:10.1016/j.exer.2010.03.002 · Cited by 98 · see citing works
Self-citationCortina, M. S., He, J., Li, N., Bazan, N. G., & Bazán, H. E. P. (2010) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.09-3641 · Cited by 89 · see citing works
Self-citationSanchez, A. C., Tripathy, D., Yin, X., et al. (2011) · Neuroscience Research · doi:10.1016/j.neures.2011.09.003 · Cited by 88 · see citing works
Jun, B., Mukherjee, P. K., Asatryan, A., Kautzmann, M.-A., Heap, J., Gordon, W. C., Bhattacharjee, S., Yang, R., Petasis, N. A., & Bazan, N. G. (2017) · Scientific Reports · doi:10.1038/s41598-017-05433-7 · Cited by 80 · see citing works
Self-citationStark, D. T., & Bazan, N. G. (2011) · Journal of Neuroscience · doi:10.1523/jneurosci.3544-11.2011 · Cited by 76 · see citing works
Self-citationChen, L., Zhang, S. S.-M., Barnstable, C. J., & Tombran‐Tink, J. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.07.188 · Cited by 75 · see citing works
Li, F., Cao, W., & Anderson, R. E. (2003) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.03-0140 · Cited by 72 · see citing works
Pigment Epithelium-derived Factor (PEDF) Prevents Retinal Cell Death via PEDF Receptor (PEDF-R)
Subramanian, P., Locatelli‐Hoops, S., Kenealey, J. D., DesJardin, J. T., Notari, L., & Becerra, S. P. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.487884 · Cited by 72 · see citing works
Antony, R., Lukiw, W. J., & Bazan, N. G. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.095232 · Cited by 69 · see citing works
Self-citationZhao, L., Liu, X., Liang, J., Han, S., Wang, Y., Yin, Y., Luo, Y., & Li, J. (2013) · Brain Research · doi:10.1016/j.brainres.2013.01.051 · Cited by 69 · see citing works
Jablonski, M. M., Tombran‐Tink, J., Mrazek, D. A., et al. (2001) · Glia · doi:10.1002/glia.1066 · Cited by 69 · see citing works
Chollangi, S., Wang, J., Martin, A. J., Quinn, J. P., & Ash, J. D. (2009) · Neurobiology of Disease · doi:10.1016/j.nbd.2009.03.012 · Cited by 64 · see citing works
Role of Lipoxygenase Metabolites in Ischemic Preconditioning
Murphy, E., Glasgow, W. C., Fralix, T. A., et al. (1995) · Circulation Research · doi:10.1161/01.res.76.3.457 · Cited by 62 · see citing works
Li, F., Cao, W., & Anderson, R. E. (2001) · Experimental Eye Research · doi:10.1006/exer.2001.1068 · Cited by 61 · see citing works
Crawford, M. J., Krishnamoorthy, R. R., Rudick, V. L., Collier, R. J., Kapin, M. A., Aggarwal, B. B., Al‐Ubaidi, M. R., & Agarwal, N. A. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.4501 · Cited by 57 · see citing works
Lu, M.-J., Chen, Y., Huang, H., et al. (2014) · Basic Research in Cardiology · doi:10.1007/s00395-014-0414-0 · Cited by 54 · see citing works
Bugge, E., & Ytrehus, K. (1996) · Cardiovascular Research · doi:10.1016/s0008-6363(96)00129-0 · Cited by 53 · see citing works
Bazan, N. G. (2007) · Springer eBooks · doi:10.1007/0-387-32442-9_74 · Cited by 51 · see citing works
Self-citationBugge, E., & Ytrehus, K. (1996) · Journal of Molecular and Cellular Cardiology · doi:10.1006/jmcc.1996.0226 · Cited by 51 · see citing works
Spatial correlation of mouse photoreceptor-RPE thickness between SD-OCT and histology
Knott, E. J., Sheets, K. G., Zhou, Y., Gordon, W. C., & Bazan, N. G. (2010) · Experimental Eye Research · doi:10.1016/j.exer.2010.10.009 · Cited by 51 · see citing works
Self-citationStarkopf, J. (1998) · Cardiovascular Research · doi:10.1016/s0008-6363(97)00240-x · Cited by 48 · see citing works
Ueki, Y., Le, Y.-Z., Chollangi, S., Müller, W., & Ash, J. D. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0906156106 · Cited by 44 · see citing works
12-Lipoxygenase in Opioid-Induced Delayed Cardioprotection
Patel, H. H., Fryer, R. M., Gross, E. R., et al. (2003) · Circulation Research · doi:10.1161/01.res.0000065167.52922.f6 · Cited by 44 · see citing works
Conservation of Docosahexaenoic Acid in the Retina
Anderson, R. E., O’Brien, P. J., Wiegand, R. D., et al. (1992) · Advances in experimental medicine and biology · doi:10.1007/978-1-4615-3426-6_25 · Cited by 40 · see citing works
ATP-sensitive potassium channels in insulinoma cells are activated by nonesterified fatty acids
Mueller, M., Szewczyk, A. M., de Weille, J. R., & Lazdunski, M. (1992) · Biochemistry · doi:10.1021/bi00134a017 · Cited by 39 · see citing works
The Effect of Ischemic Preconditioning on Light-Induced Photoreceptor Injury
Casson, R. J., Wood, J. P. M., Melena, J., et al. (2003) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.02-0368 · Cited by 37 · see citing works
Gabel, S. A., London, R. E., Funk, C. D., et al. (2001) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.2001.280.5.h1963 · Cited by 36 · see citing works
Calandria, J. M., Mukherjee, P. K., Vaccari, J. C. D. R., Zhu, M., Petasis, N. A., & Bazan, N. G. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.287078 · Cited by 30 · see citing works
Self-citationLi, X., Hong, S., Li, P., & Zhang, Y. (2010) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.110.176461 · Cited by 30 · see citing works
Protein kinase C subtypes and retinal ischemic preconditioning
Dreixler, J. C., Shaikh, A. R., Shenoy, S. K., Shen, Y., & Roth, S. (2008) · Experimental Eye Research · doi:10.1016/j.exer.2008.05.015 · Cited by 27 · see citing works
Bright cyclic rearing protects albino mouse retina against acute light-induced apoptosis.
Káldi, I., Martin, R. E., Hu, H., Brush, R. S., Morrison, K. A., & Anderson, R. E. (2003) · PubMed · Cited by 26 · see citing works
Chung, C., Doll, J. A., Stellmach, V., et al. (2008) · Advances in experimental medicine and biology · doi:10.1007/978-0-387-69080-3_61 · Cited by 23 · see citing works
Calandria, J. M., & Bazan, N. G. (2009) · Advances in experimental medicine and biology · doi:10.1007/978-1-4419-1399-9_76 · Cited by 22 · see citing works
Self-citationA cell-based assay for screening lipoxygenase inhibitors
Nair, D. G., & Funk, C. D. (2009) · Prostaglandins & Other Lipid Mediators · doi:10.1016/j.prostaglandins.2009.09.006 · Cited by 22 · see citing works
Sharma, R. K., Netland, P. A., Kedrov, M., et al. (2008) · Acta Ophthalmologica · doi:10.1111/j.1755-3768.2008.01170.x · Cited by 19 · see citing works
Liu, X., Chen, H., & Zhang, L. (2013) · PubMed · doi:10.3980/j.issn.2222-3959.2013.02.22 · Cited by 15 · see citing works
Tanito, M., Brush, R. S., Elliott, M. H., et al. (2009) · Advances in experimental medicine and biology · doi:10.1007/978-1-4419-1399-9_65 · Cited by 4 · see citing works
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The cell biology of the retinal pigment epithelium
Lakkaraju, A., Umapathy, A., Tan, L. X., Daniele, L., Philp, N. J., Boesze-Battaglia, K., & Williams, D. S. (2020) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2020.100846 · Cited by 422 · see citing works
Bazan, N. G. (2018) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2018.09.003 · Cited by 148 · see citing works
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- Authors
- Knott, E. J., Gordon, W. C., Jun, B., Do, K., & Bazan, N. G.
- Affiliations
- Louisiana State University (US)
- Corresponding author
- Bazan, Nicolas G.
- Venue
- Cellular and Molecular Neurobiology
- Published
- 2017-11-24
- Volume
- 38
- Issue
- 4
- Pages
- 901-917
- ISSN
- 0272-4340 (ISSN-L)
- 1573-6830
- PMC
- PMC5882642
- OpenAlex
- W2768758846
- Cited by
- 18
- References
- 57
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 2 · 2021: 4 · 2020: 2 · 2019: 1 · 2018: 3 · 2015: 1
- FWCI
- 1.36
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Cellular and Molecular Neurobiology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Eye Institute (EY005121)
- National Institute of General Medical Sciences (GM103340)
- Research to Prevent Blindness
- Eye, Ear, Nose & Throat Foundation
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Keywords
- retinal pigment epithelium, photoreceptors, preconditioning, docosahexaenoic acid, neuroprotectin D1, 15-lipoxygenase-1, omega-3 fatty acids, omega-6 fatty acids, arachidonic acid, eicosanoids, retinal cell survival, neuroprotection
- MeSH terms
- Animals, Arachidonate 15-Lipoxygenase, Cell Survival, Docosahexaenoic Acids, Male, Retina, Signal Transduction, Rats, Sprague-Dawley, Oxidative Stress, Neuroprotective Agents, Retinal Pigment Epithelium
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