Different effects of omega-3 fatty acids on the cell cycle in c2c12 myoblast proliferation
Abstract
Polyunsaturated fatty acids (PUFAs) are important molecules for human health. We investigated the effects of three major omega-3 PUFAs on C2C12 myoblast proliferation. Both docosahexaenoic (DHA) and eicosapentaenoic (EPA) acids decreased cell growth, whereas linolenic (ALA) acid did not, compared with the control. Cell cycle analysis showed that G(1) phase duration was increased markedly and S-phase duration was decreased by DHA and EPA. In contrast, there was no change in the G(1) or S-phase duration when the cells were treated with linolenic acid. To determine how DHA and EPA affected the cell cycle, cyclins and MAPK proteins were investigated. Western blotting and real-time quantitative PCR showed that DHA and EPA decreased cyclin E and CDK2 levels at both the protein and mRNA level. Also, MAPK phosphorylation levels were decreased by treatment with DHA and EPA. Our results indicated that different kinds of n-3 PUFA differentially affected myoblast cell proliferation. DHA and EPA decreased skeletal muscle cell proliferation through a mechanism involving MAPK-ERK.
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Exposing proliferating C2C12 cells to DHA and EPA for 24 h decreases MAPK/ERK1/2 phosphorylation, preventing progression of myoblasts from G1 to S phase.
“Exposing proliferating C2C12 cells to DHA and EPA for 24 h decreases MAPK/ERK1/2 phosphorylation, preventing the progression of myoblasts from the G1 to S phase [131].”
From Potential Roles of n-3 PUFAs during Skeletal Muscle Growth and Regeneration (2018) · cites this paper as [131]
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- Authors
- Peng, Y., Zheng, Y., Zhang, Y., Zhao, J., Chang, F., Lu, T., Zhang, R., Li, Q., Hu, X., & Li, N.
- Affiliations
- China Agricultural University (CN)
- Corresponding author
- Li, Ning
- Venue
- Molecular and Cellular Biochemistry
- Published
- 2012-05-20
- Volume
- 367
- Issue
- 1-2
- Pages
- 165-173
- ISSN
- 0300-8177 (ISSN-L)
- 1573-4919
- OpenAlex
- W2060068262
- Cited by
- 34
- References
- 46
- Citations by year
- 2026: 2 · 2025: 1 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 2 · 2014: 2 · 2013: 3
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular and Cellular Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Keywords
- omega-3 fatty acids, docosahexaenoic acid, eicosapentaenoic acid, alpha-linolenic acid, polyunsaturated fatty acids, C2C12 myoblasts, myoblast proliferation, cell cycle, G1 phase, S phase, cyclin E, CDK2, ERK signaling
- MeSH terms
- Animals, Cell Line, Cell Survival, Phosphorylation, Protein Processing, Post-Translational, Fatty Acids, Omega-3, Gene Expression, Cell Cycle Proteins, Cyclin E, Cyclin D1, MAP Kinase Signaling System, Myoblasts, Extracellular Signal-Regulated MAP Kinases, Cell Shape, Cell Proliferation, Cyclin-Dependent Kinase 2, Mice, G1 Phase Cell Cycle Checkpoints
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