Cited by 10Antifungal resistance and susceptibilityYeasts and Rust Fungi StudiesFungal Infections and Studies

Candida bracarensis: Evaluation of Virulence Factors and its Tolerance to Amphotericin B and Fluconazole

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Moreira, André, Silva, Sónia, Botelho, Cláudia, Sampaio, Paula, Pais, Célia, Henriques, Mariana·2015·Mycopathologia

Abstract

Candida bracarensis is an uncommon Candida species found during an epidemiological study of candidiasis performed in Braga, Portugal. Initially, it was identified as C. glabrata, but recently detailed analyses pointed out their differences. So, little information is still available about C. bracarensis virulence factors and antifungal susceptibilities. Therefore, the main goal of this work is to evaluate the ability of C. bracarensis to form biofilms, to produce hydrolytic enzymes (proteases, phospholipases and hemolysins), as well as its susceptibility to amphotericin B and fluconazole. It was shown, for the first time, that all C. bracarensis strains were able to form biofilms and display proteinase and hemolytic activities. Moreover, although planktonic cells presented antifungal susceptibility, amphotericin B and fluconazole were unable to inhibit biofilm formation and eradicate pre-formed biofilms. Due to the propensity of C. bracarensis to display antifungal resistance and virulence attributes, the control of these emerging pathogens is recommended.

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  1. Ru ¨chel R. Proteinases of pathogenic fungi. Mycoses. 1999;42:48-52.
  2. Al-Fattani MA, Douglas LJ. Penetration of Candida bio- films by antifungal agents. Antimicrob Agents Chemother. 2004;48(9):3291-7.

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Authors
Moreira, A., Silva, S., Botelho, C., Sampaio, P., Pais, C., & Henriques, M.
Affiliations
University of Minho (PT)
Corresponding author
Silva, Sónia
Venue
Mycopathologia
Published
2015-07-16
Volume
180
Issue
5-6
Pages
305-315
ISSN
  • 0301-486X (ISSN-L)
  • 1573-0832
OpenAlex
W859490027
Cited by
10
References
34
Citations by year
2026: 1 · 2024: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2018: 2 · 2015: 1
FWCI
0.78
Type
journal article
Language
en
Open access
Locations
  • Mycopathologia (publishedVersion)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by-nc-nd)
Funders
Fundação para a Ciência e a Tecnologia (FCOMP-01-0124-FEDER-027462, PTDC/SAU-MIC/119069/2010, FCOMP-01-0124-FEDER, PEst-OE/EQB/LA0023/2013)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
  • Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
Keywords
Candida bracarensis, virulence factors, biofilm formation, antifungal susceptibility, amphotericin B, fluconazole, proteolytic enzymes, hemolysin, phospholipase, candidiasis, emerging fungal pathogens, antifungal resistance
MeSH terms
Amphotericin B, Antifungal Agents, Candida, Candidiasis, Hemolysis, Humans, Hydrolases, Mycotoxins, Fluconazole, Biofilms, Drug Resistance, Fungal, Virulence Factors

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