Photosynthetic Physiological Performance and Proteomic Profiling of the Oleaginous Algae Scenedesmus Acuminatus Reveal the Mechanism of Lipid Accumulation under Low and High Nitrogen Supplies
Abstract
In this study, we presented cellular morphological changes, time-resolved biochemical composition, photosynthetic performance and proteomic profiling to capture the photosynthetic physiological response of Scenedesmus acuminatus under low nitrogen (3.6 mM NaNO 3, N-) and high nitrogen supplies (18.0 mM NaNO 3, N+). S. acuminatus cells showed extensive lipid accumulation (53.7% of dry weight) and were enriched in long-chain fatty acids (C16 & C18) under low nitrogen supply. The activity of PSII and photosynthetic rate decreases, whereas non-photochemical quenching and dark respiration rates were increased in the N- group. In addition, the results indicated a redistribution of light excitation energy between PSII and PSI in S. acuminatus exists before lipid accumulation. The iTRAQ results showed that, under high nitrogen supply, protein abundance of the chlorophyll biosynthesis, the Calvin cycle and ribosomal proteins decreased in S. acuminatus. In contrast, proteins associated with the photosynthetic machinery, except for F-type ATPase, were increased in the N+ group (N+, 3 vs. 9 days and 3 days, N+ vs. N-). Under low nitrogen supply, proteins involved in central carbon metabolism, fatty acid synthesis and branched-chain amino acid metabolism were increased, whereas the abundance of proteins of the photosynthetic machinery had decreased, with exception of PSI (N-, 3 vs. 9 days and 9 days, N+ vs. N-). Collectively, the current study has provided a basis for the metabolic engineering of S. acuminatus for biofuel production.
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Nitrogen limitation effectively increases TAG accumulation by redirecting captured CO2 into storage molecules such as TAGs or starch rather than proteins.
“Nitrogen limitation has been shown to effectively increase TAGs accumulation by redirecting captured CO 2 converted into storage molecules, such as TAGs or starch, rather than proteins [178].”
From A Review of Algae-Based Carbon Capture, Utilization, and Storage (Algae-Based CCUS) (2024) · cites this paper as [178]
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- Authors
- Zhang, Y., Wu, H., Sun, M., Peng, Q., & Li, A.
- Affiliations
- Jinan University (CN)
- Corresponding author
- Li, Aifen
- Venue
- Photosynthesis Research
- Published
- 2018-07-23
- Volume
- 138
- Issue
- 1
- Pages
- 73-102
- ISSN
- 0166-8595 (ISSN-L)
- 1573-5079
- OpenAlex
- W2884530966
- Cited by
- 36
- References
- 63
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 7 · 2023: 3 · 2022: 3 · 2021: 11 · 2020: 2 · 2019: 2
- FWCI
- 0.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Photosynthesis Research (publishedVersion)
- PubMed (publishedVersion)
- Institute of Hydrobiology, CAS Institutional Repository (submittedVersion)
- Funders
- The National Natural Science Foundation of China (41176105)
- The National Basic Research Fund of higher education (21614101)
- The National High Technology Research and Development Program of China (2013AA065805)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- Scenedesmus acuminatus, oleaginous microalgae, lipid accumulation, nitrogen supply, photosynthetic performance, photosystem II, non-photochemical quenching, photosystem I, proteomic profiling, long-chain fatty acids, fatty acid synthesis, Calvin cycle, chlorophyll biosynthesis, central carbon metabolism, branched-chain amino acid metabolism, biofuel production, metabolic engineering
- MeSH terms
- Amino Acids, Fatty Acids, Nitrogen, Photosynthesis, Pigments, Biological, Plant Proteins, Proteomics, Scenedesmus, Photosystem I Protein Complex, Photosystem II Protein Complex, Lipid Metabolism, Microalgae
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