The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Selection of Direct Transesterification as the Preferred Method for Assay of Fatty Acid Content of Microalgae
Abstract
Assays for total lipid content in microalgae are usually based on the Folch or the Bligh and Dyer methods of solvent extraction followed by quantification either gravimetrically or by chromatography. Direct transesterification (DT) is a method of converting saponifiable lipids in situ directly to fatty acid methyl esters which can be quantified by gas chromatography (GC). This eliminates the extraction step and results in a rapid, one‐step procedure applicable to small samples. This study compared the effectiveness of DT in quantifying the total fatty acid content in three species of microalgae to extraction using the Folch, the Bligh and Dyer and the Smedes and Askland methods, followed by transesterification and GC. The use of two catalysts in sequence, as well as the effect of reaction water content on the efficiency of DT were investigated. The Folch method was the most effective of the extraction methods tested, but comparison with DT illustrated that all extraction methods were incomplete. Higher levels of fatty acid in the cells were obtained with DT in comparison with the extraction‐transesterification methods. A combination of acidic and basic transesterification catalysts was more effective than each individually when the sample contained water. The two‐catalyst reaction was insensitive to water up to 10% of total reaction volume. DT proved a convenient and more accurate method than the extraction techniques for quantifying total fatty acid content in microalgae.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Commercial Applications of Microalgae
Spolaore, P., Joannis-Cassan, C., Duran, E., & Isambert, A. (2006) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.101.87 · Cited by 4,285
Rapid Preparation of Fatty Acid Esters from Lipids for Gas Chromatographic Analysis.
Metcalfe, L. D., Schmitz, A. A., & Pelka, J. R. (1966) · Analytical Chemistry · doi:10.1021/ac60235a044 · Cited by 2,432
Direct transesterification of all classes of lipids in a one-step reaction.
Lepage, G., & Roy, C. C. (1986) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38861-1 · Cited by 2,384
Valuable Products from Biotechnology of Microalgae
Pulz, O., & Gross, W. (2004) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-004-1647-x · Cited by 2,132 · cited 2×
“The fatty acid content of cells reflects their energy content as a feed or fuel [1, 2], as well as the yield of products such as long chain polyunsaturated fatty acids, lipid extracts for pharmaceuticals, cosmetics and nutraceuticals [ 1 , 3] and fatty acid methyl esters (FAME) for biodiesel.”
BackgroundFatty acid and lipid composition of 10 species of microalgae used in mariculture
Volkman, J. K., Jeffrey, S. W., Nichols, P. D., Rogers, G. I., & Garland, C. D. (1989) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/0022-0981(89)90029-4 · Cited by 1,044
Lepage, G., & Roy, C. C. (1984) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34457-6 · Cited by 1,021
Iverson, S. J., Lang, S. L. C., & Cooper, M. H. (2001) · Lipids · doi:10.1007/s11745-001-0843-0 · Cited by 719
Study of increasing lipid production from fresh water microalgae Chlorella vulgaris
Widjaja, A., Chien, C.-C., & Ju, Y.-H. (2009) · Journal of the Taiwan Institute of Chemical Engineers · doi:10.1016/j.jtice.2008.07.007 · Cited by 617
Cited by
Cuellar‐Bermudez, S. P., Aguilar‐Hernandez, I., Cardenas‐Chavez, D. L., Ornelas‐Soto, N., Romero‐Ogawa, M. A., & Parra‐Saldivar, R. (2015) · Microbial Biotechnology · doi:10.1111/1751-7915.12167 · Cited by 486
Griffiths, M. J., van Hille, R. P., & Harrison, S. T. L. (2012) · Journal of Applied Phycology · doi:10.1007/s10811-011-9723-y · Cited by 341
Self-citationMicroalgae bioengineering: From CO2 fixation to biofuel production
Zeng, X., Danquah, M. K., Chen, X. D., & Lu, Y. (2011) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2011.04.014 · Cited by 299
Dasan, Y. K., Lam, M. K., Yusup, S., Lim, J. W., & Lee, K. T. (2019) · Science of The Total Environment · doi:10.1016/j.scitotenv.2019.06.181 · Cited by 231
Biodiesel from mixed culture algae via a wet lipid extraction procedure
Sathish, A., & Sims, R. C. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.05.118 · Cited by 185
Metadata
- Authors
- Griffiths, M. J., van Hille, R. P., & Harrison, S. T. L.
- Venue
- Lipids
- Publisher
- Wiley
- Published
- 2010-09-05
- Volume
- 45
- Issue
- 11
- Pages
- 1053-1060
- ISSN
- 0024-4201 (ISSN-L)
- 1558-9307
- OpenAlex
- W1969963770
- Cited by
- 277
- References
- 37
- FWCI
- 5.63
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Selection of Direct Transesterification as the Preferred Method for Assay of Fatty Acid Content of Microalgae. https://app.cochat.ai/papers/10.1007/s11745-010-3468-2
- The paper
- Griffiths, M. J., van Hille, R. P., & Harrison, S. T. L. (2010). Selection of Direct Transesterification as the Preferred Method for Assay of Fatty Acid Content of Microalgae. Lipids. https://doi.org/10.1007/s11745-010-3468-2
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
