CoChat
Sign Up
Cited by 30

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Plasma exosomal miR-450b-5p as a possible biomarker and therapeutic target for transient ischaemic attacks in rats.

Luo, XiuMei, Wang, Wei, Li, DongBin, Xu, Chen, Liao, Bao, Li, FengMei, Zhou, Xia, Qin, Wu, Liu, Jingli·2019·Journal of Molecular Neuroscience
DOI 10.1007/s12031-019-01341-9
Open this paper in CoChat — free account

Abstract

Transient ischaemic attack (TIA) and cerebral infarction are difficult to identify within the thrombolytic time window. Blood markers are efficient, economical and noninvasive and can be beneficial in the diagnosis of many diseases. Plasma exosomal biomarkers are rarely reported in TIA. Exosomal microRNAs (miRNAs) were extracted from plasma and cerebrospinal fluid after middle cerebral artery occlusion (MCAo) in rats (0 min, 5 min, 10 min, 2 h). Deep sequencing was used to detect exosomal miRNAs in rat plasma and confirm significant differentially expressed miRNAs. Polymerase chain reaction (PCR) was used to detect the differentially expressed miRNAs in plasma and cerebrospinal fluid. Exosomal miRNAs with the same expression trends in plasma and cerebrospinal fluid were selected, and bioinformatics analysis was then carried out. Finally, the area under the curve (AUC) of the receiver operating characteristic (ROC) curve was determined to assess the diagnostic accuracy of miRNAs for TIA in rats. First, high-throughput sequencing was used to detect the expression level of plasma exosome miRNA, and rno-miR-450b-5p with a decreasing expression level was screened. Second, the expression levels of exosomal miRNAs were verified in cerebrospinal fluid and plasma samples by PCR, and the results indicated that exosomal rno-miR-450b-5p was similarly expressed in cerebrospinal fluid and plasma. ROC analysis showed high AUC values for rno-miR-450b-5p (0.880) in the 10 min ischaemia rats c…

Overview

Integrity signals

Registry

Crossref · 10.1007/s12031-019-01341-9

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 1 references

Research works printed but not linked: 33

  1. Ajit SK (2012) Circulating microRNAs as biomarkers, therapeutic targets, and signaling molecules. Sensors 12:3359–3369. https://doi.org/10.3390/s120303359 · DOI
  2. Arroyo JD et al (2011) Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci U S A 108:5003–5008. https://doi.org/10.1073/pnas.1019055108 · DOI · Paper page
  3. Bartel DP (2009) MicroRNAs: target recognition and regulatory functions. Cell 136:215–233. https://doi.org/10.1016/j.cell.2009.01.002 · DOI · Paper page
  4. Bushati N, Cohen SM (2007) microRNA functions. Annu Rev Cell Dev Biol 23:175–205. https://doi.org/10.1146/annurev.cellbio.23.090506.123406 · DOI · Paper page
  5. Campomenosi P et al (2016) A comparison between quantitative PCR and droplet digital PCR technologies for circulating microRNA quantification in human lung cancer. BMC Biotechnol 16:60. https://doi.org/10.1186/s12896-016-0292-7 · DOI
  6. Chalela JA, Kidwell CS, Nentwich LM, Luby M, Butman JA, Demchuk AM, Hill MD, Patronas N, Latour L, Warach S (2007) Magnetic resonance imaging and computed tomography in emergency assessment of patients with suspected acute stroke: a prospective comparison. Lancet 369:293–298. https://doi.org/10.1016/s0140-6736(07)60151-2 · DOI
  7. Chen Z, Wang K, Huang J, Zheng G, Lv Y, Luo N, Liang M, Huang L (2018) Upregulated serum MiR-146b serves as a biomarker for acute ischemic stroke. Cell Physiol Biochem 45:397–405. https://doi.org/10.1159/000486916 · DOI
  8. Cheng L, Quek CY, Sun X, Bellingham SA, Hill AF (2013) The detection of microRNA associated with Alzheimer’s disease in biological fluids using next-generation sequencing technologies. Front Genet 4:150. https://doi.org/10.3389/fgene.2013.00150 · DOI
  9. Chevillet JR, Kang Q, Ruf IK, Briggs HA, Vojtech LN, Hughes SM, Cheng HH, Arroyo JD, Meredith EK, Gallichotte EN, Pogosova-Agadjanyan EL, Morrissey C, Stirewalt DL, Hladik F, Yu EY, Higao CS, Tewari M (2014) Quantitative and stoichiometric analysis of the microRNA content of exosomes. Proc Natl Acad Sci U S A 111(41):14888–14893. https://doi.org/10.1073/pnas.1408301111 · DOI
  10. Dostie J (2003) Numerous microRNPs in neuronal cells containing novel microRNAs. Rna 9:180–186. https://doi.org/10.1261/rna.2141503 · DOI · Paper page
  11. Durukan Tolvanen A, Tatlisumak E, Pedrono E, Abo-Ramadan U, Tatlisumak T (2017) TIA model is attainable in Wistar rats by intraluminal occlusion of the MCA for 10min or shorter. Brain Res 1663:166–173. https://doi.org/10.1016/j.brainres.2017.03.010 · DOI
  12. Easton JD, Saver JL, Albers GW, Alberts MJ, Chaturvedi S, Feldmann E, Hatsukami TS, Higashida RT, Johnston SC (2009a) Definition and evaluation of transient ischemic attack: a scientific statement for healthcare professionals from the American Heart Association/American Stroke Association Stroke Council; Council on Cardiovascular Surgery and Anesthesia; Council on Cardiovascular Radiology and Intervention; Council on Cardiovascular Nursing; and the Interdisciplinary Council on Peripheral Vascular Disease. The American Academy of Neurology affirms the value of this statement as an educational tool for neurologists. Stroke. 40(6):2276–2293. https://doi.org/10.1161/STROKEAHA.108.192218 · DOI
  13. Easton JD, Saver JL, Albers GW, Alberts MJ, Chaturvedi S, Feldmann E, Hatsukami TS, Higashida RT, Johnston SC, Kidwell CS, Lutsep HL, Miller E, Sacco RL, American Heart Association, American Stroke Association Stroke Council, Council on Cardiovascular Surgery and Anesthesia, Council on Cardiovascular Radiology and Intervention, Council on Cardiovascular Nursing, Interdisciplinary Council on Peripheral Vascular Disease (2009b) Definition and evaluation of transient ischemic attack: a scientific statement for healthcare professionals from the American Heart Association/American Stroke Association Stroke Council; Council on Cardiovascular Surgery and Anesthesia; Council on Cardiovascular Radiology and Intervention; Council on Cardiovascular Nursing; and the Interdisciplinary Council on Peripheral Vascular Disease. The American Academy of Neurology affirms the value of this statement as an educational tool for neurologists. Stroke 40:2276–2293. https://doi.org/10.1161/STROKEAHA.108.192218 · DOI
  14. Fan Y, Zhang C, Li T, Peng W, Yin J, Li X, Kong Y, Lan C, Wang R, Hu Z (2016) A new approach of short wave protection against middle cerebral artery occlusion/reperfusion injury via attenuation of Golgi apparatus stress by inhibition of downregulation of secretory pathway Ca(2+)-ATPase isoform 1 in rats. J Stroke Cerebrovasc Dis 25(7):1813–1822 doi: 10.1016/j · DOI
  15. Ganzer CA, Barnes A, Uphold C, Jacobs AR (2016) Transient ischemic attack and cognitive impairment: a review. J Neurosci Nurs 48:322–327. https://doi.org/10.1097/JNN.0000000000000236 · DOI
  16. Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T (2002) Identification of tissue specific microRNAs from mouse. Curr Biol 12(9):735–739 · DOI · Paper page
  17. Li C, Pei F, Zhu X, Duan DD, Zeng C (2012) Circulating microRNAs as novel and sensitive biomarkers of acute myocardial infarction. Clin Biochem 45:727–732. https://doi.org/10.1016/j.clinbiochem.2012.04.013 · DOI
  18. Li M et al (2018) All-trans retinoic acid ameliorates the early experimental cerebral ischemia-reperfusion injury in rats by inhibiting the loss of the blood-brain barrier via the JNK/P38MAPK signaling pathway. Neurochem Res 43:1283–1296. https://doi.org/10.1007/s11064-018-2545-4 · DOI
  19. Liang Z, Chi YJ, Lin GQ, Luo SH, Jiang QY, Chen YK (2018) MiRNA-26a promotes angiogenesis in a rat model of cerebral infarction via PI3K/AKT and MAPK/ERK pathway. Eur Rev Med Pharmacol Sci 22(11):3485–3492. https://doi.org/10.26355/eurrev_201806_15175. · DOI
  20. Lin Z, Murtaza I, Wang K, Jiao J, Gao J, Li PF (2009) mi R-23a functions downstream of NFATc3 toregulate cardiac hypertrophy[J]. Proc Natl Acad Sci U S A 106(29):12103–12108 · DOI
  21. Ma Z et al (2016) MicroRNA-155 expression inversely correlates with pathologic stage of gastric cancer and it inhibits gastric cancer cell growth by targeting cyclin D1. J Cancer Res Clin Oncol 142:1201–1212. https://doi.org/10.1007/s00432-016-2139-y · DOI
  22. Mandriota SJ, Menoud PAa, Pepper MS (1996) Transforming growth factor b 1 down-regulates vascular endothelial growth factor receptor 2/flk-1 expression in vascular endothelial cells. J Biol Chem 271(19):11500–11505. https://doi.org/10.1074/jbc.271.19.11500 · DOI
  23. Miska EA, Alvarez-Saavedra E, Townsend M, Yoshii A, Sestan N, Rakic P, Constantine-Paton M, Horvitz HR (2004) Microarray analysis of microRNA expression in the developing mammalian brain. Genome Biol 5(9):R68. https://doi.org/10.1186/gb-2004-5-9-r68 · DOI · Paper page
  24. Pedrono E, Durukan A, Strbian D, Marinkovic I, Shekhar S, Pitkonen M, Abo-Ramadan U, Tatlisumak T (2010) An optimized mouse model for transient ischemic attack. J Neuropathol Exp Neurol 69(2):188–195. https://doi.org/10.1097/NEN.0b013e3181cd331c · DOI
  25. Podlesniy P, Trullas R (2018) Biomarkers in cerebrospinal fluid: analysis of cell-free circulating mitochondrial DNA by digital PCR. Methods Mol Biol 1768:111–126. https://doi.org/10.1007/978-1-4939-7778-9_7 · DOI
  26. Racki N, Dreo T, Gutierrez-Aguirre I, Blejec A, Ravnikar M (2014) Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples. Plant Methods 10:42. https://doi.org/10.1186/s13007-014-0042-6 · DOI · Paper page
  27. Shao S et al (2017) Atorvastatin attenuates ischemia/reperfusion-induced hippocampal neurons injury via Akt-nNOS-JNK signaling pathway. Cell Mol Neurobiol 37:753–762. https://doi.org/10.1007/s10571-016-0412-x · DOI
  28. Shono K, Satomi J, Tada Y, Kanematsu Y, Yamamoto N, Izumi Y, Kaji R, Harada M, Nagahiro S (2017) Optimal timing of diffusion-weighted imaging to avoid false-negative findings in patients with transient ischemic attack. Stroke 48:1990–1992. https://doi.org/10.1161/STROKEAHA.117.014576 · DOI
  29. Sun MM et al (2014) TGF-beta1 suppression of microRNA-450b-5p expression: a novel mechanism for blocking myogenic differentiation of rhabdomyosarcoma. Oncogene 33:2075–2086. https://doi.org/10.1038/onc.2013.165 · DOI
  30. Sun H, Zhao J, Zhong D, Li G (2017) Potential serum biomarkers and metabonomic profiling of serum in ischemic stroke patients using UPLC/Q-TOF MS/MS. PLoS One 12:e0189009. https://doi.org/10.1371/journal.pone.0189009 · DOI
  31. Wang H, Zhang XM, Tomiyoshi G, Nakamura R, Shinmen N, Kuroda H, Kimura R, Mine S, Kamitsukasa I, Wada T, Aotsuka A, Yoshida Y, Kobayashi E, Matsutani T, Iwadate Y, Sugimoto K, Mori M, Uzawa A, Muto M, Kuwabara S, Takemoto M, Kobayashi K, Kawamura H, Ishibashi R, Yokote K, Ohno M, Chen PM, Nishi E, Ono K, Kimura T, Machida T, Takizawa H, Kashiwado K, Shimada H, Ito M, Goto KI, Iwase K, Ashino H, Taira A, Arita E, Takiguchi M, Hiwasa T (2017) Association of serum levels of antibodies against MMP1, CBX1, and CBX5 with transient ischemic attack and cerebral infarction. Oncotarget. 9(5):5600–5613. https://doi.org/10.18632/oncotarget.23789 eCollection 2018 Jan 19 · DOI
  32. Yagi Y, Ohkubo T, Kawaji H, Machida A, Miyata H, Goda S, Roy S, Hayashizaki Y, Suzuki H, Yokota T (2017) Next-generation sequencing-based small RNA profiling of cerebrospinal fluid exosomes. Neurosci Lett 636:48–57. https://doi.org/10.1016/j.neulet.2016.10.042 · DOI
  33. Ye YP, Wu P, Gu CC, Deng DL, Jiao HL, Li TT, Wang SY, Wang YX, Xiao ZY, Wei WT, Chen YR, Qiu JF, Yang RW, Lin J, Liang L, Liao WT, Ding YQ (2016) miR-450b-5p induced by oncogenic KRAS is required for colorectal cancer progression. Oncotarget. 7(38):61312–61324. https://doi.org/10.18632/oncotarget.11016 · DOI

Cited by

Sorted by citations

See all 30

Metadata

Authors
Luo, X., Wang, W., Li, D., Xu, C., Liao, B., Li, F., Zhou, X., Qin, W., & Liu, J.
ORCID
Liu, Jingli
Venue
Journal of Molecular Neuroscience
Publisher
Springer Science and Business Media LLC
Published
2019-07-31
Volume
69
Issue
4
Pages
516-526
ISSN
  • 0895-8696
  • 1559-1166
DOI
10.1007/s12031-019-01341-9
Cited by
30
References
1
Language
en
Licence
Funders
National Natural Science Foundation of China (81660354)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Plasma exosomal miR-450b-5p as a possible biomarker and therapeutic target for transient ischaemic attacks in rats. https://app.cochat.ai/papers/10.1007/s12031-019-01341-9
The paper
Luo, X., Wang, W., Li, D., Xu, C., Liao, B., Li, F., Zhou, X., Qin, W., & Liu, J. (2019). Plasma exosomal miR-450b-5p as a possible biomarker and therapeutic target for transient ischaemic attacks in rats. Journal of Molecular Neuroscience. https://doi.org/10.1007/s12031-019-01341-9