Cited by 103Neurological Disorders and TreatmentsFatty Acid Research and HealthNeuroinflammation and Neurodegeneration Mechanisms

Docosanoids promote neurogenesis and angiogenesis, blood-brain barrier integrity, penumbra protection, and neurobehavioral recovery after experimental ischemic stroke

Open access
Belayev, Ludmila, Hong, Sung-Ha, Menghani, Hemant, Marcell, Shawn J., Obenaus, Andre, Freitas, Raul S., Khoutorova, Larissa, Balaszczuk, Veronica +3 more·2018·Molecular Neurobiology

Abstract

Docosahexaenoic acid (DHA) and neuroprotectin D1 (NPD1) are neuroprotective after experimental ischemic stroke. To explore underlying mechanisms, SD rats underwent 2 h of middle cerebral artery occlusion (MCAo) and treated with DHA (5 mg/kg, IV) or NPD1 (5 μg/per rat, ICV) and vehicles 1 h after. Neuro-behavioral assessments was conducted on days 1, 2, and 3, and on week 1, 2, 3, or 4. BrdU was injected on days 4, 5, and 6, immunohistochemistry was performed on week 2 or 4, MRI on day 7, and lipidomic analysis at 4 and 5 h after onset of stroke. DHA improved short- and long-term behavioral functions and reduced cortical, subcortical, and total infarct volumes (by 42, 47, and 31%, respectively) after 2 weeks and reduced tissue loss by 50% after 4 weeks. DHA increased the number of BrdU+/Ki-67+, BrdU+/DCX+, and BrdU+/NeuN+ cells in the cortex, subventricular zone, and dentate gyrus and potentiated NPD1 synthesis in the penumbra at 5 h after MCAo. NPD1 improved behavior, reduced lesion volumes, protected ischemic penumbra, increased NeuN, GFAP, SMI-71-positive cells and vessels, axonal regeneration in the penumbra, and attenuated blood-brain barrier (BBB) after MCAo. We conclude that docosanoid administration increases neurogenesis and angiogenesis, activates NPD1 synthesis in the penumbra, and diminishes BBB permeability, which correlates to long-term neurobehavioral recovery after experimental ischemic stroke.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1007/s12035-018-1136-3

Cites

61 of 61 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 62 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Belayev, L., Hong, S.-H., Menghani, H., Marcell, S. J., Obenaus, A., Freitas, R. S., Khoutorova, L., Balaszczuk, V., Jun, B., Oriá, R. B., & Bazan, N. G.
Affiliations
Louisiana State University (US)
Corresponding author
Belayev, Ludmila
Venue
Molecular Neurobiology
Published
2018-06-01
Volume
55
Issue
8
Pages
7090-7106
ISSN
  • 0893-7648 (ISSN-L)
  • 1559-1182
OpenAlex
W2807382073
Cited by
103
References
62
Citations by year
2026: 10 · 2025: 11 · 2024: 13 · 2023: 13 · 2022: 15 · 2021: 17 · 2020: 17 · 2019: 6 · 2018: 1
FWCI
4.60
Type
journal article
Language
en
Open access
Locations
  • Molecular Neurobiology (publishedVersion)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • PubMed Central (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
  • CONICET Digital (CONICET) (submittedVersion, cc-by-nc-sa)
Funders
  • National Institute of Neurological Disorders and Stroke (NS046741)
  • National Institute of General Medical Sciences (GM103340)
Topics
  • Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
docosanoids, docosahexaenoic acid, neuroprotectin D1, ischemic stroke, neurogenesis, angiogenesis, blood-brain barrier integrity, neurobehavioral recovery, middle cerebral artery occlusion, infarct size, axon regeneration, subventricular zone, dentate gyrus, lipidomics
MeSH terms
Doublecortin Protein, Animals, Axons, Behavior, Animal, Blood-Brain Barrier, Brain Ischemia, Docosahexaenoic Acids, Fatty Acids, Male, Nerve Regeneration, Permeability, Survival Analysis, Rats, Sprague-Dawley, Neovascularization, Physiologic, Infarction, Middle Cerebral Artery, Stroke, Neurogenesis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review