Cited by 89Probiotics and Fermented FoodsReproductive tract infections researchInfant Nutrition and Health

Antibacterial and Antibiofilm Activity of Lactic Acid Bacteria Isolated from Traditional Artisanal Milk Cheese from Northeast China Against Enteropathogenic Bacteria

Cui, Xingyang, Shi, Yunjia, Gu, Shanshan, Yan, Xin, Chen, Hongyan, Ge, Junwei·2018·Probiotics and Antimicrobial Proteins

Abstract

The present study aims to investigate the probiotic properties of novel strains of lactic acid bacteria isolated from traditional artisanal milk cheese from Northeast China and to explore their antibacterial activity against enteropathogenic bacteria. Of the 321 isolates, 86 exhibited survival in low pH, resistance to pancreatin, and tolerance to bile salts; of these, 12 inhibited the growth of more than seven enteropathogenic bacteria and exhibited antibiofilm activities against Staphylococcus aureus CMCC26003 and/or Escherichia coli CVCC230. Based on 16S ribosomal RNA sequence analysis, the 12 isolates were assigned to Lactobacillus plantarum (7), Lactobacillus helveticus (3), Pediococcus acidilactici (1), and Enterococcus faecium (1) species. In addition, 5 of the 12 strains were susceptible to most of the tested antibiotics. Furthermore, four strains with sensitivity to antibiotics showed significantly high levels of hydrophobicity similar to or better than the reference strain Lactobacillus rhamnosus GG. Moreover, three strains were confirmed safe through non-hemolytic activities and bacterial translocation. Overall, the selected Lact. plantarum 27053 and 27172 and Lact. helveticus 27058 strains can be considered potential probiotic strains and candidates for further application in functional food and prevention or treatment of gastrointestinal diseases.

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Crossref · 10.1007/s12602-017-9364-9

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Authors
Cui, X., Shi, Y., Gu, S., Yan, X., Chen, H., & Ge, J.
Affiliations
  • Northeast Agricultural University (CN)
  • Chinese Academy of Agricultural Sciences (CN)
Corresponding author
Ge, Junwei
Venue
Probiotics and Antimicrobial Proteins
Published
2017-12-07
Volume
10
Issue
4
Pages
601-610
ISSN
  • 1867-1306 (ISSN-L)
  • 1867-1314
OpenAlex
W2771717832
Cited by
88
References
45
Citations by year
2026: 4 · 2025: 12 · 2024: 8 · 2023: 20 · 2022: 15 · 2021: 14 · 2020: 6 · 2019: 4 · 2018: 4
FWCI
4.31
Type
journal article
Language
en
Open access
closed
Locations
  • Probiotics and Antimicrobial Proteins (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Natural Science Foundation of Heilongjiang Province (LC2015006)
  • National Natural Science Foundation of China (31672532)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
Keywords
lactic acid bacteria, probiotic strains, antibiofilm activity, antibacterial activity, enteropathogenic bacteria, Staphylococcus aureus, Escherichia coli, Lactobacillus plantarum, Lactobacillus helveticus, Pediococcus acidilactici, Enterococcus faecium, hydrophobicity, bile salt tolerance, antibiotic susceptibility, bacterial translocation, functional foods, gastrointestinal diseases
MeSH terms
Animals, Antibiosis, Cattle, Cheese, China, Escherichia coli, Escherichia coli Infections, Milk, Staphylococcal Infections, Staphylococcus aureus, Biofilms, Probiotics, Mice, Lactobacillales

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