Direct projection from the suprachiasmatic nucleus to hypophysiotrophic corticotropin-releasing factor immunoreactive cells in the paraventricular nucleus of the hypothalamus demonstrated by means ofPhaseolus vulgaris-leucoagglutinin tract tracing

Vrang, Niels; Larsen, Philip J.; Mikkelsen, Jens D.

doi:10.1016/0006-8993(95)00425-p

Cited by 123Circadian rhythm and melatoninStress Responses and CortisolHypothalamic control of reproductive hormones

Direct projection from the suprachiasmatic nucleus to hypophysiotrophic corticotropin-releasing factor immunoreactive cells in the paraventricular nucleus of the hypothalamus demonstrated by means ofPhaseolus vulgaris-leucoagglutinin tract tracing

Vrang, Niels, Larsen, Philip J., Mikkelsen, Jens D.·1995·Brain Research

DOI 10.1016/0006-8993(95)00425-p

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Abstract

The diurnal rhythm of the activity of the hypothalamo-pituitary-adrenal axis is generated by the circadian pacemaker located in the suprachiasmatic nuclei (SCN). However, the neuronal circuit connecting the SCN with the neurosecretory corticotropin-releasing factor (CRF) neurons in the paraventricular nucleus of the hypothalamus is not clear. To investigate the existence of a direct link between the SCN and the CRF neurons in the PVN we combined microiontopheretic injections of the anterograde tracer Phaseolus vulgaris-leucoagglutinin (PHA-L) into the SCN with immunohistochemical detection of CRF in adrenalectomized male rats. The majority of the PHA-L-ir axons originating from the SCN terminated in the subparaventricular area. A minor contingent of fibers continued into the PVN proper, involving the medial and dorsal parvicellular subnuclei of the PVN. All PHA-L injections involving the entire SCN gave rise to PHA-L positive fibers endowed with boutons en passage and terminal boutons contacting CRF positive cell bodies in the PVN. Notably, varicosities on the PHA-L labelled fibers were present in close proximity to cell bodies and proximal dendrites of a subportion of the CRF neurons located in the periphery of the CRF cell cluster. The present study provides the first evidence to suggest a direct connection between the SCN and the CRF producing neurons of the hypothalamo-pituitary-adrenocortical axis in the PVN. Considering the sparse number of PHA-L-ir varicosities in close proximity to the CRF-ir cells, it seems likely that this direct pathway constitutes but a part of a projection system from the SCN, possibly involving multisynaptic pathways, influencing the hypothalamo-pituitary-adrenocortical axis.

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The SCN has direct and indirect projections to the PVN.
“The SCN shows, indeed, direct and indirect projections (mainly via the subparaventricular zone and the dorsomedial nucleus of the hypothalamus) to the PVN [17, 18].”
From Impact of Sleep and Its Disturbances on Hypothalamo-Pituitary-Adrenal Axis Activity (2010) · cites this paper as [17]

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Authors: Vrang, N., Larsen, P. J., & Mikkelsen, J. D.
Affiliations: University of Copenhagen (DK)
Corresponding author: Vrang, Niels
ORCID: Vrang, Niels
Mikkelsen, Jens D.
Venue: Brain Research
Publisher: Elsevier BV
Published: 1995-06
Volume: 684
Issue: 1
Pages: 61-69
ISSN: 0006-8993 (ISSN-L)
DOI: 10.1016/0006-8993(95)00425-p
OpenAlex: W1992566076
Cited by: 123
References: 36
Citations by year: 2025: 2 · 2024: 1 · 2023: 1 · 2022: 5 · 2021: 9 · 2020: 5 · 2019: 6 · 2018: 5 · 2017: 3 · 2016: 6 · 2015: 2 · 2014: 5 · 2013: 4 · 2012: 4
FWCI: 1.68
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Brain Research (publishedVersion)
PubMed (publishedVersion)
Funders: Sundhed og Sygdom, Det Frie Forskningsråd (12-1064, 12-1642-2)
Lægeforeningen
Novo Nordisk Fonden
Topics: Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
Keywords: suprachiasmatic nucleus, corticotropin-releasing factor, paraventricular nucleus, hypothalamic-pituitary-adrenal axis, circadian rhythm, varicosities, adrenalectomy, rats
MeSH terms: Animals, Brain Mapping, Circadian Rhythm, Corticotropin-Releasing Hormone, Immunohistochemistry, Male, Neural Pathways, Paraventricular Hypothalamic Nucleus, Phytohemagglutinins, Pituitary Gland, Suprachiasmatic Nucleus, Rats, Wistar, Rats

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Direct projection from the suprachiasmatic nucleus to hypophysiotrophic corticotropin-releasing factor immunoreactive cells in the paraventricular nucleus of the hypothalamus demonstrated by means ofPhaseolus vulgaris-leucoagglutinin tract tracing

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