Template-directed synthesis on the pentanucleotide CpCpGpCpC
Abstract
The pentanucleotide CpCpGpCpC facilitates the synthesis of oligomers containing G and C from a mixture of the two activated mononucleotides (guanosine 5'-phosphor)-2-methylimidazolide and (cytidine 5'-phosphor)-2-methylimidazolide. The major pentameric product of the template-directed reaction is all 3' to 5'-linked and has the sequence pGpGpCpGpG, which is complementary to that of the template. It can be obtained in a yield of up to 17%, based on the input of the template. The 3' to 5' isomer of GpG is elongated on the template to give GpGpC, GpGpCpG and GpGpCpGpG, while the 2' to 5' isomer does not initiate the synthesis of detectable amounts of longer oligomers.
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Inoue et al. showed by experiment 35 years ago that RNA could be formed by condensation of nucleotide imidazolides on a template.
“As an example, Inoue et al. showed by experiment 35 years ago that RNA could be formed by condensation of nucleotide imidazolides on a template12.”
From Prebiotic plausibility and networks of paradox-resolving independent models (2018) · cites this paper as [12]
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- Authors
- Inoue, T., Joyce, G. F., Grzeskowiak, K., Orgel, L. E., Brown, J. M., & Reese, C. B.
- Affiliations
- Salk Institute for Biological Studies (US)
- King's College London (GB)
- ORCID
- Venue
- Journal of Molecular Biology
- Publisher
- Elsevier BV
- Published
- 1984-09
- Volume
- 178
- Issue
- 3
- Pages
- 669-676
- ISSN
- 0022-2836 (ISSN-L)
- OpenAlex
- W1996889491
- Cited by
- 80
- References
- 7
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 2 · 2021: 3 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 3 · 2016: 1 · 2014: 1 · 2012: 2
- FWCI
- 1.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chemical Synthesis and Analysis
- Keywords
- oligonucleotide synthesis, nucleotide activation
- MeSH terms
- Chromatography, High Pressure Liquid, Cytidine Monophosphate, Guanosine Monophosphate, Oligonucleotides, Templates, Genetic
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