Cited by 92Origins and Evolution of LifeRNA and protein synthesis mechanismsDNA and Nucleic Acid Chemistry

The limits of template-directed synthesis with nucleoside-5′-phosphoro(2-methyl)imidazolides

Hill, Aubrey R., Orgel, Leslie E., Wu, Taifeng·1993·Origins of life and evolution of the biosphere

Abstract

In earlier work we have shown that C-rich templates containing isolated A, T or G residues and short oligo(G) sequences can be copied effectively using nucleoside-5'-phosphoro(2-methyl)imidazolides as substrates. We now show that isolated A or T residues within an oligo(G) sequence are a complete block to copying and that an isolated C residue is copied inefficiently. Replication is possible only if there are two complementary oligonucleotides each of which acts as a template to facilitate the synthesis of the other. We emphasize the severity of the problems that need to be overcome to make possible non-enzymatic replication in homogeneous aqueous solution. We conclude that an efficient catalyst was involved in the origin of polynucleotide replication.

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Crossref · 10.1007/bf01582078

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8 of 8 references verified0 citation errors · 0 not checkable

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  • Enzyme-free primer extension initially explored by Orgel and colleagues faced significant limitations, including low yield, prolonged reaction time and poor fidelity.

    “Initially explored by Orgel and colleagues to understand the origins of life, this method faced significant limitations, including low yield, prolonged reaction time and poor fidelity.8”

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Authors
Hill, A. R., Orgel, L. E., & Wu, T.
Affiliations
  • Salk Institute for Biological Studies (US)
  • Northwestern University (US)
Venue
Origins of life and evolution of the biosphere
Published
1993-12
Volume
23
Issue
5-6
Pages
285-290
ISSN
  • 0169-6149 (ISSN-L)
  • 1573-0875
OpenAlex
W2065742859
Cited by
92
References
8
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2022: 5 · 2021: 2 · 2020: 1 · 2019: 7 · 2018: 2 · 2017: 2 · 2016: 5 · 2015: 10 · 2014: 3 · 2013: 2 · 2012: 2
FWCI
3.60
Type
journal article
Language
en
Open access
closed
Locations
  • Origins of Life and Evolution of Biospheres (publishedVersion)
  • PubMed (publishedVersion)
  • NASA STI Repository (National Aeronautics and Space Administration) (submittedVersion)
Funders
National Aeronautics and Space Administration
Topics
  • Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
template synthesis
MeSH terms
Base Sequence, DNA, DNA Replication, Models, Biological, Molecular Sequence Data, Nucleic Acid Conformation, Nucleotides, Oligodeoxyribonucleotides

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