Complement gene expression in human brain: comparison between normal and Alzheimer disease cases
Abstract
Many investigators have reported the presence of complement proteins in Alzheimer disease (AD) brains, but their origin is uncertain. We report the expression of complement genes C1q, C3 and C4 in RNA extracted from temporal cortex samples of post-mortem human brain. The transcripts for C3 and C4 were detected by Northern hybridization analysis, and the mRNAs for C1q, C3 and C4 were detected by polymerase chain reaction (PCR) amplification of brain derived complementary DNA (cDNA). The relative abundances of mRNAs for C3 and C4 were compared between samples of temporal cortex from neurologically normal and Alzheimer disease (AD) cases, using a semiquantitative PCR assay. There was a 3.01 fold mean increase in expression of C3 and a 3.27-fold mean increase in expression of C4 in AD samples compared to control cases. These results indicate that localized synthesis of some of the major complement components can occur in human brain. The factors causing activation of complement in AD still remain undetermined.
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Strong evidence has implicated the complement system in the pathogenesis of Alzheimer's disease.
“Strong evidence has implicated the complement system in the pathogenesis of Alzheimer’s disease [72–76]”
Cites
McGeer, P. L., Itagaki, S., Tago, H., & McGeer, E. G. (1987) · Neuroscience Letters · doi:10.1016/0304-3940(87)90696-3 · Cited by 836 · see citing works
Self-citationBrain microglia constitutively express β-2 integrins
Akiyama, H., & McGeer, P. L. (1990) · Journal of Neuroimmunology · doi:10.1016/0165-5728(90)90055-r · Cited by 430 · see citing works
Self-citationActivation of the classical complement pathway in brain tissue of Alzheimer patients
McGeer, P. L., Akiyama, H., Itagaki, S., & McGeer, E. G. (1989) · Neuroscience Letters · doi:10.1016/0304-3940(89)90843-4 · Cited by 383 · see citing works
Self-citationMay, P. C., Lampert-Etchells, M., Johnson, S. A., Poirier, J., Masters, J. N., & Finch, C. E. (1990) · Neuron · doi:10.1016/0896-6273(90)90342-d · Cited by 339 · see citing works
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Inflammation and Alzheimer’s disease
Akiyama, H. (2000) · Neurobiology of Aging · doi:10.1016/s0197-4580(00)00124-x · Cited by 4,514 · see citing works
MCGEER, P., & MCGEER, E. (1995) · Brain Research Reviews · doi:10.1016/0165-0173(95)00011-9 · Cited by 1,287 · see citing works
Self-citationComplement activation by beta-amyloid in Alzheimer disease.
Rogers, J., Cooper, N. R., Webster, S., Schultz, J., McGeer, P. L., Styren, S. D., Civin, W. H., Brachova, L., Bradt, B., & Ward, P. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.21.10016 · Cited by 817 · see citing works
Self-citationAnderson, D. H., Radeke, M. J., Gallo, N. B., Chapin, E. A., Johnson, P. T., Curletti, C. R., Hancox, L. S., Hu, J., Ebright, J. N., Malek, G., et al. (2010) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2009.11.003 · Cited by 727 · see citing works
Microglia and inflammatory mechanisms in the clearance of amyloid β peptide
Rogers, J., Strohmeyer, R., Kovelowski, C. J., & Li, R. (2002) · Glia · doi:10.1002/glia.10153 · Cited by 381 · see citing works
Age related macular degeneration and drusen: Neuroinflammation in the retina
Buschini, E., Piras, A., Nuzzi, R., & Vercelli, A. (2011) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2011.05.011 · Cited by 230 · see citing works
Complement in the pathogenesis of Alzheimer’s disease
Morgan, B. P. (2018) · Seminars in Immunopathology · doi:10.1007/s00281-017-0662-9 · Cited by 194 · see citing works
The role of complement and activated microglia in the pathogenesis of Alzheimer's disease
Eikelenboom, P., & Veerhuis, R. (1996) · Neurobiology of Aging · doi:10.1016/0197-4580(96)00108-x · Cited by 206 · see citing works
Barber, A. J., del Genio, C. L., Swain, A. B., Pizzi, E. M., Watson, S. C., Tapiavala, V. N., Zanazzi, G. J., & Gaur, A. B. (2024) · Alzheimer's Research & Therapy · doi:10.1186/s13195-024-01492-x · Cited by 41 · see citing works
- Authors
- Walker, D. G., & McGeer, P. L.
- Affiliations
- University of British Columbia (CA)
- Corresponding author
- Walker, Douglas G.
- Venue
- Molecular Brain Research
- Publisher
- Elsevier BV
- Published
- 1992-06
- Volume
- 14
- Issue
- 1-2
- Pages
- 109-116
- ISSN
- 0169-328X (ISSN-L)
- OpenAlex
- W2020508042
- Cited by
- 167
- References
- 4
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 16 · 2023: 5 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 5 · 2017: 3 · 2016: 1 · 2015: 2 · 2013: 1 · 2012: 1
- FWCI
- 4.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Complement system in diseases
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- complement system, Alzheimer's disease, complement gene expression, C1q, complement C3, C4, temporal cortex, mRNA expression, postmortem brain, complement activation
- MeSH terms
- Aged, Aged, 80 and over, Alzheimer Disease, Amino Acid Isomerases, Base Sequence, Brain, Carrier Proteins, Complement Pathway, Classical, Complement C3, Complement C4, Female, Humans, Male, Middle Aged, Molecular Sequence Data, RNA, Messenger, Gene Expression, Complement C1q, Polymerase Chain Reaction, Peptidylprolyl Isomerase
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