Effects of iontophoretically applied monoamines on somatosensory cortical neurons of unanesthetized rats
Abstract
The response of somatosensory cortical neurons to iontophoretic applications of monoamines was studied in unanesthetized rats. The animal's head was held in a stereotaxic apparatus by means of a painless head-restraining system implanted 8 days prior to the recording sessions. The electrodes consisted of a recording micropipette attached to a multibarreled iontophoresis micropipette. The electrode penetrations were reconstructed on camera lucida drawings of frontal brain sections. The percentage of cortical neurons responding to application of monoamines were 76% after noradrenaline, 58% after dopamine and 66% after serotonin. The differences observed among percentages of responses seemingly correlate with the relative abundance of terminal axons and receptors for each of the three monoamines in the somatosensory cortex. The vast majority of the responding neurons were inhibited by monoamines and this inhibitory effect was independent of the level of spontaneous activity. The depressant effect of the monoamines on glutamate and acetylcholine-evoked responses supports a modulatory role for these substances. Serotonin was the most potent, followed by noradrenaline and dopamine. The present study shows that when the influence of anesthesia is eliminated, the predominant effect of monoamines on cortical first somatosensory neurons is one of inhibition. These findings contrast with results obtained under some anesthetic conditions, as well as under in vitro conditions.
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Local application of noradrenaline or stimulation of noradrenergic afferents in vivo reduces spontaneous firing.
“Local application of noradrenaline or stimulation of noradrenergic afferents in vivo reduces spontaneous firing194,195, but enhances evoked responses196–198”
From Mechanisms underlying gain modulation in the cortex (2020) · cites this paper as [195]
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- Authors
- Bassant, M. H., Ennouri, K., & Lamour, Y.
- Affiliations
- Inserm (FR)
- Corresponding author
- Bassant, M.H.
- Venue
- Neuroscience
- Publisher
- Elsevier BV
- Published
- 1990-01
- Volume
- 39
- Issue
- 2
- Pages
- 431-439
- ISSN
- 0306-4522 (ISSN-L)
- OpenAlex
- W2036281305
- Cited by
- 51
- References
- 57
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2016: 1 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 0.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- monoamines, somatosensory cortex, iontophoresis, norepinephrine, dopamine, serotonin, cortical neurons, neuronal inhibition, anesthetic effects, unanesthetized rats
- MeSH terms
- Acetylcholine, Animals, Dopamine, Evoked Potentials, Glutamates, Iontophoresis, Male, Neurons, Norepinephrine, Rats, Inbred Strains, Serotonin, Somatosensory Cortex, Rats
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