The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Subpopulations of gabaergic neurons in laminae i–iii of rat spinal dorsal horn defined by coexistence with classical transmitters, peptides, nitric oxide synthase or parvalbumin
Abstract
GABAergic neurons in laminae I-III of the spinal dorsal horn may contain one or more of the following compounds: glycine, acetylcholine, neuropeptide Y, enkephalin, nitric oxide synthase or parvalbumin. Although the pattern of co-localization of some of these compounds is understood, it is not known which types of GABAergic neurons contain parvalbumin, or whether nitric oxide synthase coexists with peptides, acetylcholine or parvalbumin in any of these neurons, and in this study we have used immunocytochemistry and enzyme histochemistry to resolve these issues. Parvalbumin-immunoreactivity was restricted to those GABA-immunoreactive neurons that also showed glycine-immunoreactivity and was not co-localized with neuropeptide Y-immunoreactivity or NADPH diaphorase activity. By combining NADPH diaphorase histochemistry with immunocytochemistry with an antiserum to nitric oxide synthase, we were able to show that NADPH diaphorase activity was a reliable marker for nitric oxide synthase in the spinal cord. Neurons that possess GABA- but not glycine-immunoreactivity may contain neuropeptide Y, enkephalin, acetylcholine or NADPH diaphorase, and all of the cholinergic neurons appear to contain NADPH diaphorase. By combining immunofluorescent detection of neuropeptide Y or enkephalin with NADPH diaphorase histochemistry, we showed that peptide-immunoreactivity did not coexist with NADPH diaphorase. This suggests that neither of these peptides coexists with nitric oxide synthase or wit…
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“The Chat dorsal inhibitor cluster Inhib-14 was a deep dorsal (lamina 4), inhibitory and cholinergic population and also expressed Nos180–82.”
From A harmonized atlas of mouse spinal cord cell types and their spatial organization (2021) · cites this paper as [80]
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Cites
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Self-citationParvalbumin in Most γ-Aminobutyric Acid-Containing Neurons of the Rat Cerebral Cortex
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Self-citationThe Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn
Abraira, V. E., Kuehn, E. D., Chirila, A. M., Springel, M. W., Toliver, A. A., Zimmerman, A. L., Orefice, L. L., Boyle, K. A., Bai, L., Song, B. J., et al. (2017) · Cell · doi:10.1016/j.cell.2016.12.010 · Cited by 409
“Similar to the rat dorsal horn, we found that approximately 70% of PV interneurons in laminae I–III contain GABA and glycine (Hughes et al., 2012; Laing et al., 1994).”
Müller, T., Brohmann, H., Pierani, A., Heppenstall, P. A., Lewin, G. R., Jessell, T. M., & Birchmeier, C. (2002) · Neuron · doi:10.1016/s0896-6273(02)00689-x · Cited by 368
Foster, E., Wildner, H., Tudeau, L., Haueter, S., Ralvenius, W. T., Jegen, M., Johannssen, H. C., Hösli, L., Haenraets, K., Ghanem, A., et al. (2015) · Neuron · doi:10.1016/j.neuron.2015.02.028 · Cited by 366
Dorsal Horn Parvalbumin Neurons Are Gate-Keepers of Touch-Evoked Pain after Nerve Injury
Petitjean, H., Pawlowski, S. A., Fraine, S. L., Sharif, B., Hamad, D., Fatima, T., Berg, J., Brown, C. M., Jan, L.-Y., Ribeiro-da-Silva, A., et al. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.09.080 · Cited by 326
Metadata
- Authors
- Laing, I., Todd, A. J., Heizmann, C. W., & Schmidt, H. H. H. W.
- Affiliations
- University of Glasgow (GB)
- University of Zurich (CH)
- Universitätsklinikum Würzburg (DE)
- Corresponding author
- Todd, A.J.
- Venue
- Neuroscience
- Publisher
- Elsevier BV
- Published
- 1994-07
- Volume
- 61
- Issue
- 1
- Pages
- 123-132
- ISSN
- 0306-4522 (ISSN-L)
- OpenAlex
- W2029706214
- Cited by
- 177
- References
- 55
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 2 · 2021: 9 · 2020: 8 · 2019: 4 · 2018: 5 · 2017: 9 · 2016: 3 · 2015: 5 · 2014: 4 · 2013: 10 · 2012: 1
- FWCI
- 4.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Topics
- Health Sciences › Medicine › Physiology (medical) › Pain Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- GABAergic neurons, dorsal horn, parvalbumin, nitric oxide synthase, NADPH-diaphorase, neuropeptide Y, enkephalin, acetylcholine, glycine, neuropeptide co-localization, inhibitory interneurons, cholinergic neurons, rats
- MeSH terms
- Acetylcholine, Amino Acid Oxidoreductases, Animals, Enkephalins, Female, gamma-Aminobutyric Acid, Glycine, Immunohistochemistry, Male, NADPH Dehydrogenase, Neurons, Neuropeptide Y, Neuropeptides, Parvalbumins, Spinal Cord, Biomarkers, Rats, Sprague-Dawley, Neurotransmitter Agents, Nitric Oxide Synthase, Mice, Rats
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