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Cited by 304Optical Imaging and Spectroscopy TechniquesNeuroscience and Neuropharmacology ResearchNeural dynamics and brain function

Imaging cell volume changes and neuronal excitation in the hippocampal slice

Andrew, R.D., Macvicar, B.A.·1994·Neuroscience
DOI 10.1016/0306-4522(94)90372-7
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Abstract

Brain cell swelling is a consequence of seizure, ischemia or excitotoxicity. Changes in light reflectance from cortical surface are now used to monitor brain activity but these intrinsic signals are poorly understood. The objectives of this study were first, to show that changes in light transmittance were correlated with cell volume and second, to image increases in light transmittance as they related to neuronal activation. Transverse hippocampal slices from the rat were used for the study. Brief exposure (4-6 min) to hypo-osmotic artificial cerebrospinal fluid (-40 mOsm) elevated light transmittance consistently and reversibly in most regions of the slice and particularly in CA1 dendritic regions. Neither zero-Ca2+ artificial cerebrospinal fluid nor tetrodotoxin altered the transmittance increase and its subsequent reversal, suggesting that it was dependent on osmolality but independent of synaptic transmission and neuronal firing. The amplitude of the CA1 population spike evoked from Schaffer collaterals increased concomitantly with the hypo-osmotic increase in light transmittance, providing evidence that the extracellular tissue resistance increased. Hyper-osmotic artificial cerebrospinal fluid (+40 mOsm) containing impermeant mannitol consistently lowered light transmittance and the amplitude of the population spike. Glycerol (+40 mOsm), which is cell permeant, did not have an affect. Taken together these observations indicate that osmotic challenge alters light transmi…

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  • Neuronal activation is accompanied by transient swelling of neurons, dendritic spines, and axons, as well as cytoskeletal rearrangements, together with extracellular-space modulation driven by depolarization, ion fluxes, and osmotic shifts.

    “Neuronal activation is accompanied by transient swelling of neurons, dendritic spines, and axons, as well as cytoskeletal rearrangements (Andrew & MacVicar, 1994; Cohen et al., 1968; El Hady & Machta, 2015; Iwasa & Tasaki, 1980; Iwasa et al., 1980; Kim et al., 2007; MacVicar & Hochman, 1991; Tasaki, 1999; Tasaki & Byrne, 1990), together with extracellular-space modulation driven by depolarization,”

    From 40 Years of diffusion MRI in the brain: From history to emerging frontiers (2026) · cites this paper as Andrew & MacVicar, 1994

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Authors
Andrew, R. D., & Macvicar, B. A.
Affiliations
  • Queen's University (CA)
  • University of Calgary (CA)
Corresponding author
Andrew, R.D.
ORCID
Venue
Neuroscience
Publisher
Elsevier BV
Published
1994-09
Volume
62
Issue
2
Pages
371-383
ISSN
0306-4522 (ISSN-L)
DOI
10.1016/0306-4522(94)90372-7
OpenAlex
W2051143596
Cited by
304
References
39
Citations by year
2026: 3 · 2025: 4 · 2024: 9 · 2023: 3 · 2022: 5 · 2021: 9 · 2020: 4 · 2019: 5 · 2018: 12 · 2017: 9 · 2016: 6 · 2015: 8 · 2014: 11 · 2013: 6 · 2012: 14
FWCI
10.34
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
Keywords
optical transmittance, cell volume changes, neuronal excitation, hippocampal slices, osmotic challenge, cell swelling, CA1, population spike, kainic acid, action potential, excitotoxicity, glial swelling, tetrodotoxin, antidromic stimulation, glycerol, stratum radiatum, stratum oriens
MeSH terms
Animals, Axons, Brain Edema, Dendrites, Electric Stimulation, Evoked Potentials, Glycerol, Hippocampus, Hypotonic Solutions, Kainic Acid, Male, Models, Neurological, Neurons, Rats, Sprague-Dawley, Pyramidal Cells, Rats, In Vitro Techniques

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