Imaging White Adipose Tissue with Confocal Microscopy
Open accessAbstract
Adipose tissue is composed of a variety of cell types that include mature adipocytes, endothelial cells, fibroblasts, adipocyte progenitors, and a range of inflammatory leukocytes. These cells work in concert to promote nutrient storage in adipose tissue depots and vary widely based on location. In addition, overnutrition and obesity impart significant changes in the architecture of adipose tissue that are strongly associated with metabolic dysfunction. Recent studies have called attention to the importance of adipose tissue microenvironments in regulating adipocyte function and therefore require techniques that preserve cellular interactions and permit detailed analysis of three-dimensional structures in fat. This chapter summarizes our experience with the use of laser scanning confocal microscopy for imaging adipose tissue in rodents.
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The size of immune cell clusters can indicate the inflammatory state within tissues, with larger clusters correlating with a more activated and pro-inflammatory state.
“The size of immune cell clusters can indicate the inflammatory state within tissues, with larger clusters correlating with a more activated and pro-inflammatory state24.”
From A deep-learning framework reveals whole-body perturbations at cell level (2026) · cites this paper as [24]
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“Imaging of whole-mount white adipose tissue followed the method described by Martinez-Santibañez et al. (2014) 65 .”
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- Authors
- Martinez-Santibañez, G., Cho, K. W., & Lumeng, C. N.
- Affiliations
- University of Michigan (US)
- Corresponding author
- Lumeng, Carey N.
- Venue
- Methods in Enzymology
- Publisher
- Elsevier
- Published
- 2014
- Volume
- 537
- Pages
- 17-30
- ISSN
- 0076-6879 (ISSN-L)
- PMC
- PMC4233125
- OpenAlex
- W337374803
- Cited by
- 64
- References
- 33
- Citations by year
- 2026: 6 · 2025: 4 · 2024: 6 · 2023: 4 · 2022: 5 · 2021: 8 · 2020: 6 · 2019: 10 · 2018: 5 · 2017: 3 · 2016: 5 · 2015: 1 · 2014: 1
- FWCI
- 8.55
- Type
- book chapter
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- University of Michigan
- National Institutes of Health
- Nutrition Obesity Research Center, University of North Carolina
- National Institute of General Medical Sciences (T32GM007315)
- National Institute of Diabetes and Digestive and Kidney Diseases (P60DK020572, R21DK092873, DK089503, R01DK090262, DK092873, DK090262, P30DK089503)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
- Keywords
- white adipose tissue, confocal microscopy, adipose tissue imaging, adipocytes, adipose tissue microenvironment, obesity, adipose tissue inflammation, adipose tissue depots, 3D imaging
- MeSH terms
- Cell Differentiation, Humans, Macrophages, Obesity, T-Lymphocytes, Adipocytes, Microscopy, Confocal, Adipose Tissue, White, Cellular Microenvironment
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