Advances in molecular diagnosis of malaria

Zhi Jian Zheng; Zhibin Cheng

doi:10.1016/bs.acc.2016.11.006

Cited by 42Malaria Research and ControlBiosensors and Analytical DetectionBird parasitology and diseases

Advances in molecular diagnosis of malaria

Zhi Jian Zheng, Zhibin Cheng·2017·Advances in Clinical Chemistry

DOI 10.1016/bs.acc.2016.11.006

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Abstract

Malaria is a mosquito-borne disease caused by five species of Plasmodium parasites. Accurate diagnosis of malaria plays an essential part in malaria control. With traditional diagnostic methodologies, malaria control programs have achieved remarkable success during the past decade, and are now heading toward malaria elimination in many areas. This new situation, however, calls for novel diagnostics with improved sensitivity, throughput, and reduced cost for active screening of malaria parasites, as all transfected individuals have to be identified in order to block transmission. In this chapter, we provide a brief introduction of malaria, the requirement of diagnostic advances in the age of malaria elimination, and a comprehensive overview of the currently available molecular malaria diagnostics, ranging from well-known tests to platforms in early stages of evaluation. We also discussed several practical issues for the application of molecular tests in malaria identification.

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Active case detection strategy has proven ineffective in cases of low endemicity and asymptomatic infections.
“This strategy has proven ineffective in cases of low endemicity and asymptomatic infections.”
From Diagnostic Accuracy of a Thick Blood Smear Compared to qPCR for Malaria Associated with Pregnancy in Colombia (2023) · cites this paper as [33]
A community trial in Burkina Faso with 12 months of follow-up demonstrated that active detection strategy has no effect on the prevalence of Plasmodium spp. or the incidence of malaria.
“a community trial in Burkina Faso with 12 months of follow-up demonstrated that active detection strategy has no effect on the prevalence of Plasmodium spp. or the incidence of malaria [33,37] owing to the low sensitivity of the diagnosis.”
From Diagnostic Accuracy of a Thick Blood Smear Compared to qPCR for Malaria Associated with Pregnancy in Colombia (2023) · cites this paper as [33]
Other authors have estimated that the thick blood smear only detects and controls 20-50% of cases.
“Other authors have estimated that TBS only detects and controls 20–50% of cases [33,36].”
From Diagnostic Accuracy of a Thick Blood Smear Compared to qPCR for Malaria Associated with Pregnancy in Colombia (2023) · cites this paper as [33]

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Authors: Zheng, Z. J., & Cheng, Z.
Affiliations: Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
Corresponding author: Zhi Jian Zheng
ORCID: Zhi Jian Zheng
Zhibin Cheng
Venue: Advances in Clinical Chemistry
Publisher: Elsevier
Published: 2017
Volume: 80
Pages: 155-192
ISSN: 0065-2423 (ISSN-L)
DOI: 10.1016/bs.acc.2016.11.006
OpenAlex: W2589203238
Cited by: 41
References: 77
Citations by year: 2026: 1 · 2025: 4 · 2024: 5 · 2023: 13 · 2022: 7 · 2021: 3 · 2020: 4 · 2019: 1 · 2018: 2 · 2017: 1
FWCI: 11.47
Type: book chapter
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Advances in clinical chemistry (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
Life Sciences › Immunology and Microbiology › Parasitology › Bird parasitology and diseases
Keywords: malaria, molecular diagnosis, Plasmodium, malaria elimination, malaria screening, transfusion-transmitted malaria, diagnostic sensitivity, malaria control
MeSH terms: Humans, Malaria, Sensitivity and Specificity, Biomarkers, Nucleic Acid Amplification Techniques

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