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Cited by 476Malaria Research and ControlBiosensors and Analytical DetectionSARS-CoV-2 detection and testing

Sensitive and Inexpensive Molecular Test for Falciparum Malaria: Detecting Plasmodium falciparum DNA Directly from Heat-Treated Blood by Loop-Mediated Isothermal Amplification,

Poon, Leo LM, Wong, Bonnie WY, Ma, Edmund HT, Chan, Kwok H, Chow, Larry MC, Abeyewickreme, Wimal, Tangpukdee, Noppadon, Yuen, Kwok Y +3 more·2006·Clinical Chemistry
DOI 10.1373/clinchem.2005.057901
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Abstract

Background: Malaria is one of the most important parasitic infections in humans. A sensitive diagnostic test for malaria that could be applied at the community level could be useful in programs to control the disease. The aim of the present work was to develop a simple, inexpensive molecular test for Plasmodium falciparum. Methods: Blood was collected from controls (n = 100) and from patients diagnosed with falciparum malaria infection (n = 102), who were recruited to the study. Heat-treated blood samples were tested by a loop-mediated isothermal amplification (LAMP) assay for P. falciparum. Results were interpreted by a turbidity meter in real time or visually at the end of the assay. To evaluate the assay, DNA from these samples was purified and tested by PCR. Results from the LAMP and PCR assays were compared. Results: The LAMP assay detected P. falciparum directly from heat-treated blood. The quantitative data from the assay correlated to the parasite counts obtained by blood-film microscopic analyses. When we used the PCR assay as the comparison method, the sensitivity and specificity of the LAMP assay were 95% and 99%, respectively. Conclusions: Unlike PCR, the LAMP assay does not require purified DNA for efficient DNA amplification, thereby reducing the cost and turnaround time for P. falciparum diagnosis. The assay requires only basic instruments, and assay positivity can be verified by visual inspection.

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Crossref · 10.1373/clinchem.2005.057901

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  • LAMP is a newer alternative to PCR and qPCR that does not require DNA purification, uses simple instrumentation, and can be completed in less than 1 hour.

    “Loop-mediated isothermal amplification (LAMP) is a newer alternative to PCR and qPCR. This method does not require DNA purification, utilizes simple instrumentation (water bath or a heat block), and can be completed in less than 1 h [33,34].”

    From Diagnosing malaria in pregnancy: An update (2012) · cites this paper as [33]

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Authors
Poon, L. L., Wong, B. W., Ma, E. H., Chan, K. H., Chow, L. M., Abeyewickreme, W., Tangpukdee, N., Yuen, K. Y., Guan, Y., Looareesuwan, S., & Peiris, J. M.
Affiliations
  • University of Hong Kong (HK)
  • Department of Microbiology, The University of Hong Kong, Hong Kong SAR
  • Hong Kong Polytechnic University (HK)
  • University of Kelaniya (LK)
Corresponding author
Poon, Leo LM
ORCID
Venue
Clinical Chemistry
Publisher
Oxford University Press (OUP)
Published
2006-02-01
Volume
52
Issue
2
Pages
303-306
ISSN
  • 0009-9147 (ISSN-L)
  • 1530-8561
DOI
10.1373/clinchem.2005.057901
OpenAlex
W2135086664
Cited by
476
References
15
Citations by year
2026: 7 · 2025: 10 · 2024: 22 · 2023: 14 · 2022: 17 · 2021: 25 · 2020: 24 · 2019: 29 · 2018: 24 · 2017: 29 · 2016: 36 · 2015: 41 · 2014: 44 · 2013: 28 · 2012: 31
FWCI
8.98
Type
journal article
Language
en
Open access
closed
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations
  • Clinical Chemistry (publishedVersion)
  • PubMed (publishedVersion)
  • The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
  • PolyU Institutional Research Archive (Hong Kong Polytechnic University) (submittedVersion)
Funders
  • Research Grant Council (HKU 7343/04M)
  • University of Hong Kong
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
  • Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
  • Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 detection and testing
Keywords
falciparum malaria, Plasmodium falciparum, loop-mediated isothermal amplification, malaria diagnosis, point-of-care testing, low-cost molecular diagnostics, parasite detection, sensitivity and specificity, PCR comparison
MeSH terms
Animals, Base Sequence, Calibration, Hot Temperature, Humans, Molecular Sequence Data, Plasmodium falciparum, Sensitivity and Specificity, Specimen Handling, DNA, Protozoan, Polymerase Chain Reaction, Malaria, Falciparum

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